The objective of this paper was to assay the effect of different oils supplementation on dynamics of gas production and coenzyme F420 of ruminal microbe in vitro, using 3 Xuhuai white goats with permanent cannulas. Five groups were designed as follows: control group (no oil), peanut oil (A), rapeseed oil (B), corn oil (C), and soybean oil (D) respectively. Each group had 3 replicates and 1 fermentation bottle per replicate. The results showed as follows: 1) the volume of accumulated gas within 36 h was varied from 20.61 to 39.67 mL, except for the peanut oil group, the other oil groups were all significantly lower than the control group (P<0.05); the gas production of the 5 groups showed fluctuation with time prolonging and significant differences were found in methane production and methane to gas production ratio among groups (P<0.05), and methane to gas production ratio had a decreasing tendency from B to D. 2) Coenzyme F420 fluorescence value of the control group was higher than that of the oil groups (P<0.05), except for the rapeseed oil group, coenzyme F420 fluorescence value decreased with increasing degrees of oil unsaturation. 3) Good correlations were observed between coenzyme F420 fluorescence value and methane production (r=0.916), coenzyme F420 fluorescence value and gas production (r=0.905) (2-tail<0.01). 4) The total dehydrogenase activity of the soybean oil group was significantly higher than that of the control group, and was also higher than that of the corn oil, peanut oil and rapeseed oil groups in order (P<0.05). Finally, it could be concluded that, the inhibiting effects of the oil supplementation on coenzyme F420 fluorescence value, methane and gas production enhanced with increasing unsaturation degrees of the four kinds of oils, and soybean oil showed a remarkable effect comparatively.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1819 -1825］
WANG Mengzhi,WANG Shu,PAN Xiaohua,WANG Hongrong,WANG Jiaqi
. Supplementation of Four Different Oils Affects Gas Production and Coenzyme F420 of Ruminal Microbe in Vitro[J]. Chinese Journal of Animal Nutrition, 2011
, 23(10)
: 1819
-1825
.
DOI: 10.3969/j.issn.1006-267x.2011.10.024
［1］HOLTER J B, YOUNG A J. Methane production in dry and lactating Holstein cows[J]. Journal of Dairy Science, 1992, 72:2165-2175.
[2]TAKAHASHI J. Nutritional manipulation of methanogenesis in ruminants[J]. Journal of Animal Science, 2001, 14:131-135.
[3]BUSQUET M, CALSAMIGLIA S, FERRET A, et al. Effect of garlic oil and four of its compounds on rumen microbial fermentation[J]. Journal of Dairy Science, 2005, 88:4393-4404.
[4]JALCˇ D, CˇERTIK M, KUNDRIKOVA K, et al. Effect of microbial oil and fish oil on rumen fermentation and metabolism of fatty acids in artificial rumen[J]. Czech Journal of Animal Science, 2009, 54(5):229-237.
[5]OLDICK B S, FIRKINS J L. Effects of degree of fat saturation on fiber digestion and microbial protein synthesis when diets are fed twelve times daily[J]. Journal of Animal Science, 2000, 78:2412-2420.
[6]NHAN N T H, NGU N T, THIET N, et al. Determination of the optimum level of a soybean oil drench with respect to the rumen ecosystem, feed intake and digestibility in cattle[J]. Livestock Research for Rural Development. 2007, 19(8):117-118.
[7]WANG M Z, WANG H R, YU L H. Effects of NDF Content on Protozoal Community and Grazing Rate in Rumen[J]. Journal of Animal and Veterinary Advances, 2009, 8(9):1746-1752.
[8]MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research and Development, 1988, 28:7-55.
[9]俞毓馨，吴国庆，孟宪庭．环境工程微生物检验手册[M]．北京：中国环境科学出版社，1990．
[10]HUMEYAN D B, NAGARAJA T G, MILLER G W, et al. Rumen microbial changes in cattle fed diets with or without salinomycin[J]. Applied and Environmental Microbiology, 1986, 51(2):340-345.
[11]FAICHNEY G J. Rumen physiology: the key to understanding the conversion of plants into animal products[J]. Australian Journal of Agricultural Research, 1996, 47(2):163-174.
[12]PEN B, SAR C, MWENYA B, et al. Effects of Yucca schidigera and Quillajasaponaria extracts on in vitro ruminal fermentation and methane emission[J]. Animal Feed Science and Technology, 2006, 129:175-186.
[13]SALLAM S M A, BUENO I C S, BRIGIDE P, et al. Efficacy of eucalyptus oil on in vitro ruminal fermentation and methane production[J]. Options Méditerranéennes, Nutritional and Foraging Ecology of Sheep and Goats, 2009, Series A (85):267-272.
[14]TOKURA M, CHAGAN I, USHIDA K, et al. Phylogenetic study of methanogens associated with rumen ciliates[J]. Current Microbiology, 1999, 39:123-128.
[15]JALCˇ D, POTKANSKI A, SZUMACHER-STRABEL M, et al. Effect of microbial oil, evening primrose oil on rumen fermentation in vitro[J]. Veterinary Medicine Czech, 2005, 50 (11):480-486.
[16]JALCˇ D, CˇEREKOV Z. Effect of plant oils and malate on rumen fermentation in vitro[J]. Czech Journal of Animal Science, 2002, 47(3):106-111.
[17]AVILA C D, DE PETERS E J, PEREZ-MONTI H, et al. Influences of saturation ratio of supplemental dietary fat on digestion and milk yield in dairy cows[J]. Journal of Dairy Science, 2000, 83:1505-1519.
[18]陈蛋，陈斌，陆道礼，等．近红外光谱分析法测定菜籽油中芥酸的含量[J]．农业工程学报，2007，23(1):234-237．
[19]TZENG S F, WOLFE R S, BRYANT M P. Factor 420-dependent tyridine nucleotide-linked hydrogenase system of Methanobacterium ruminantium[J]. Journal of Bacteriology, 1975, 121(1):184-191.
[20]DOLFING J, WILLEM J. Comparison of methane production rate and coenzyme F420 content of methanogenic consortia in anaerobic granular sludge[J]. Applied and Environmental Microbiology, 1985, 49(5):1142-1145.
