Dietary Energy Level Affects Lipid Catabolism-Related Gene Expression in Adipose Tissue of Wujin Pigs

PAN Hongbin, WANG Jing, HUANG Ying, ZHAO Sumei, ZHANG Xi, GE Changrong, GAO Shizheng

doi:10.3969/j.issn.1006-267x.2011.11.015

Chinese Journal of Animal Nutrition >

2011 , Vol. 23 >Issue 11: 1946 - 1952

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.11.015

Molecular Nutrition

Dietary Energy Level Affects Lipid Catabolism-Related Gene Expression in Adipose Tissue of Wujin Pigs

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Yunnan Key Laboratory of Animal Nutrition and Feed Science, Yunnan Agricultural University, Kunming 650201, China

Received date: 2011-05-11

Online published: 2011-11-21

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Abstract

The aim of this study was to investigate the effects of dietary energy level on the expression of lipid catabolism-related genes in adipose tissue of Wujin pigs. Fifty-four Wujin pigs with body weight of 15 kg were randomly assigned into 3 groups with 3 replicates per group and 6 heads in each replicate. Pigs in low digestive energy group (11.74 MJ/kg, LDE group), middle digestive energy group (12.89 MJ/kg, MDE group) and high digestive energy group (14.22 MJ/kg, HDE group) were fed diets with three different digestive energy levels. At the body weight of 30, 60 and 100 kg, pigs were slaughtered and subcutaneous adipose tissue was collected for analysis of gene expression levels of lipid catabolism-related enzymes and factors by real-time PCR. The results showed as follows: at the body weight of 30 and 100 kg, the gene expression levels of HSL, CPT-Ⅰ and PPARγ in adipose tissue of Wujin pigs fed high energy level diet significantly decreased (P<0.05), while the gene expression level of LPL significantly increased(P<0.05); however, at the body weight of 60 kg, the expression levels of the four genes were significantly increased (P<0.05). The results indicate that high dietary energy level can decrease lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 30 and 100 kg, but enhance lipid catabolism and fatty acid β-oxadation of Wujin pigs at the body weight of 60 kg.

Key words： Wujin pigs; dietary energy level; adipose tissue; lipid catabolism; gene expression

Cite this article

PAN Hongbin, WANG Jing, HUANG Ying, ZHAO Sumei, ZHANG Xi, GE Changrong, GAO Shizheng . Dietary Energy Level Affects Lipid Catabolism-Related Gene Expression in Adipose Tissue of Wujin Pigs[J]. Chinese Journal of Animal Nutrition, 2011 , 23(11) : 1946 -1952 . DOI: 10.3969/j.issn.1006-267x.2011.11.015

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