Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening, Identification and Effects on Degradation of Forage Fiber

DONG Jieli, ZHANG Tieying, QI Zhiguo, WU Xingqun, OUYANG Zhangzhi

doi:10.3969/j.issn.1006-267x.2011.11.016

2011 , Vol. 23 >Issue 11: 1953 - 1960

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.11.016

Feed Science and Technology

Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening, Identification and Effects on Degradation of Forage Fiber

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1. State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science, Nanjing Agriculture University, Nanjing 210095, China;
3. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received date: 2011-06-10

Online published: 2011-11-21

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Abstract

This experiment was conducted to study the isolation, screening and identification of efficient xylan-degrading bacteria from the hindgut of high roughage-resistance Inner Mongolia black adult sows, and to compare the effects of screening bacteria and rumen liquid on neutral detergent fiber (NDF) and acid detergent fiber (ADF) degradation of forages (alfalfa, Chinese wildrye and silage corn). Using xylan as the sole carbon source, two xylan-degrading bacteria named ZF and MZ were isolated by screening with the diameter of clearing zone and xylanase activity. Strains of ZF and MZ were identified as Cellulomonas flavigena and Paenibacillus sp., respectively, which were facultative anaerobe strains, by morphology observation and 16S rDNA sequence analysis. The degradation results showed that the degradabilities of NDF and ADF in alfalfa and silage corn by rumen fluid were higher than those by strains of ZF and MZ. With fermentation time prolonging, the degradabilities of NDF and ADF in Chinese wildrye by strain ZF and MZ had a tendency to increase, and the degradabilities of NDF and ADF in Chinese wildrye by strain ZF were equal or higher than those by rumen fluid. In conclusion, xylana-degrading bacteria ZF and MZ isolated from the hindgut of adult sows have efficient ability to degrade fiber; therefore, they can be used as candidate strains for xylanase production.

Key words： adult sows; xylan-degrading bacteria; screening; identification; fiber degradation

Cite this article

DONG Jieli, ZHANG Tieying, QI Zhiguo, WU Xingqun, OUYANG Zhangzhi . Xylan-Degrading Bacteria from the Hindgut of Adult Sows: Screening, Identification and Effects on Degradation of Forage Fiber[J]. Chinese Journal of Animal Nutrition, 2011 , 23(11) : 1953 -1960 . DOI: 10.3969/j.issn.1006-267x.2011.11.016

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