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Protective Effects of Pomegranate Peel Polyphenol Extract against Related Harmful Factors of Subacute Ruminal Acidosis

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  • Institute of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China

Received date: 2011-05-14

  Online published: 2011-11-21

Abstract

This study established a in vitro subacute ruminal acidosis (SARA) model to evaluate the effects of different supplemental levels of pomegranate peel polyphenols extract (PPPE) on related harmful factors of subacute ruminal acidosis. Single factor design was used for the trial, and 3 supplemental levels of PPPE were design, which were 0, 4.5% and 9.0% of fermentation substrate, respectively. Each supplemental level had 5 replicates. The results showed as follows: with prolonged fermentation time, the pH of fermentation broth was gradually decreased. The PPPE supplementation could retard the pH decreased, and the pH of fermentation broth at the 6th, 12th and 18th h in the group containing 4.5% PPPE was significantly higher than that in the group containing 9.0% PPPE (P<0.05). The contents of endotoxin and histamine in the fermentation broth were significantly decreased by PPPE supplementation (P<0.05), and showed a dose-dependant effect. After supplemented PPPE, the volatile fatty acid contents were all decreased to different extent, and significant effects were found in some time points (P<0.05). The results indicate that PPPE supplementation can effectively relieve SARA, and the 4.5% supplemental level has a better effect.

Cite this article

DU Yu, WANG Zhisheng, DONG Lifeng . Protective Effects of Pomegranate Peel Polyphenol Extract against Related Harmful Factors of Subacute Ruminal Acidosis[J]. Chinese Journal of Animal Nutrition, 2011 , 23(11) : 2031 -2036 . DOI: 10.3969/j.issn.1006-267x.2011.11.026

References

[1] BEAUCHEMIN K A. Ruminal acidosis in dairy cows: balancing physically effective fiber with starch availability //Institute of food and agricultural sciences: proceedings of the 18th florida ruminant nutrition symposium. Gainesville: IFAS, 2007:16-27.

[2] DARREN W B. Effect of the number of step-up diets fed during grain adaptation on acidosis and feeding behaviour of feedlot cattle . Master thesis. Saskatoon: University of Saskatchewan, 2005.

[3] ASCHENBACH J R, GABEL G. Effect and absorption of histamine in sheep rumen: significance of acidotic epithelial damage[J]. Journal Animal Science, 2000, 78:464-470.

[4] KHAFIPOUR E, KRAUSE D O, PLAIZIER J C. A grain-based subacute ruminal acidosis challenge causes translocation of lipopolysaccharide and triggers inflammation[J]. Journal Dairy Science, 2008, 92:1060-1070.

[5] BADI M T, CHAJI M, ESLAMI M, et al. The evaluation of the effect of tannin of oak leave on in vitro rumen fermentation of soybean meal[J]. Research Journal of Biological Sciences, 2009, 4(11):1190-1192.

[6] NJIDDA A A. In vitro gas production and stoichiometric relationship between short chain fatty acid and in vitro gas production of semi-arid browses of north-eastern Nigeria[J]. Global Veterinaria, 2010, 4(3):292-298.

[7] BADI M T, CHAJI M, TABATABAEI S. The effect of tannic acid on in vitro gas production and rumen fermentation of sunflower meal[J]. Journal of Animal and Veterinary Advances, 2010, 9(2):277-280.  

[8] MENKE K H, STEINGASS H. Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid[J]. Animal Research Development, 1988, 28:7-55.

[9] 丁学智,龙瑞军,淡瑞芳,等.瘤胃发酵液挥发性脂肪酸的气相色谱分析方法[J].甘肃农业大学学报,2006,4(2):24-26.

[10] EHSAN K, LI S C, JAN C P, et al. Rumen microbiome composition determined using two nutritional models of subacute ruminal acidosis[J]. Applied and Environmental Microbiology, 2009, 75(22):7115-7124.  

[11] OETZEL G R. Introduction to ruminal acidosis in dairy cattle //Proceedings of the 34th annual convention of American association of bovine practitioners. Columbus: AABP, 2001:15-17.

[12] VALENTINA V, HARINDER P S, MARCELLO M, et al. Ruminal biohydrogenation as affected by tannins in vitro[J]. British Journal of Nutrition, 2009, 102:82-92.

[13] GETACHEW G, PITTROFF W, DEPETERS E J, et al. Influence of tannic acid application on alfalfa hay: in vitro rumen fermentation, serum metabolites and nitrogen balance in sheep[J].Animal, 2008, 2(3):381-390.
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