This paper was conducted to investigate the effects of acetate to propionate ratio on the pattern of volatile fatty acid (VFA) fermentation and diversities of rumen microorganisms. Four goats fitted with permanent ruminal cannulas were used to provide rumen fluid for the in vitro study. The four treatments were set according to the acetate to propionate ratio in culture substrates, which were 30∶70, 50∶0, 70∶30, and 100∶0, respectively. Variation of VFA concentration was recorded, and diversities of bacteria and protozoa were investigated by SSCP fingerprint technique. The results showed as follows: acetate concentration of 100∶0 group was the highest and was significantly higher than that of the other three groups (P<0.05), while that of 50∶50 group was the lowest. Propionate concentration of 30∶70 group was the highest and was significantly higher than that of groups 50∶50 and 70∶30 (P<0.05). Butyrate concentration of 30∶70 group was significantly higher than that of 70∶30 group (P<0.05). Acetate to propionate ratios in culture medium of all the treatments were close to 3∶1, while that of 30∶70 group was significantly lower than that of groups 70∶30 and 100∶0 (P<0.05). Bacterial SSCP fingerprint showed that bands number of 50∶50 group was the most, while that of 100∶0 group was the least; protozoa SSCP fingerprint showed that bands number of 100∶0 group was the most, while that of 30∶70 group was the least. Similarity index was the highest between 50∶50 group and 70∶30 group on both bacteria and protozoa fingerprints. In conclusion, the VFA pattern and diversities of rumen bacterial or protozoal community can be modified by acetate to propionate ratio in vitro.
YIN Zhaohua, WANG Mengzhi, WANG Hongrong, ZHANG Jie, YU Lihuai
. Acetate to Propionate Ratio: Effects on Volatile Fatty Acid Fermentation Pattern and Microorganisms Diversities in Rumen Fluid in Vitro[J]. Chinese Journal of Animal Nutrition, 2011
, 23(12)
: 2129
-2135
.
DOI: 10.3969/j.issn.1006-267x.2011.12.013
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