This study was conducted to evaluate the effects of different dietary lipid sources on body lipid deposition, fatty acid composition, and activities of lipid metabolism enzymes and digestive enzymes of Carassius auratus gibelio. Five hundred and twenty-five healthy Carassius auratus gibelio with an average initial weight of （6.04±0.05) g were randomly divided into 5 groups with 3 replicates per group and 35 fish per replicate. Five experimental diets were formulated to contain 4% lipid originated from fish oil, soybean oil, lard, peanut oil and mixed oil (fish oil︰soybean oil︰lard=3︰4︰3), respectively. The feeding trial lasted for 60 days. The results showed as follows: lipid content in hepatopancreas of fish oil group was significantly lower than that of the other groups (P<0.05); whereas, no significant difference was observed in abdominal lipid rate and muscle lipid content among all groups (P>0.05). The activities of lipoprotein lipase and hepatic lipase in hepatopancreas of fish oil group were significantly higher than those of lard and peanut oil groups (P<0.05). Protease activity in intestine and hepatopancreas of fish oil and soybean oil groups was significantly higher than that of lard group (P<0.05), but not significantly different from that of the rest groups (P>0.05). Hepatopancreas lipase activity of soybean oil and mixed oil groups was significantly higher than that of lard group (P<0.05), and the intestine lipid activity of mixed oil group was significantly higher than that of lard group (P<0.05), but no significant difference was observed among other groups (P>0.05). No significant difference was observed in intestine and hepatopancreas amylase activities among all the groups (P>0.05), but the amylase activity in intestine was generally higher than that in hepatopancreas.The centents of saturated fatty acid (SFA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in muscle and hepatopancreas of fish oil group were significantly higher than those of the other groups (P<0.05). In addition, the highest linoleic acid content in muscle and hepatopancreas was found in soybean oil group while the lowest in fish oil group, and significant difference was found between the above two groups (P<0.05). The results indicate that fish oil can enhance the activities of hepatopancreas lipoprotein lipase and hepatic lipase of Carassius auratus gibelio with relatively low body lipid deposition as a consequence, whereas the effects of the lard are on the opposite; and the fatty acid composition in tissues of Carassius auratus gibelio is influenced by dietary fatty acid composition.［Chinese Journal of Animal Nutrition，2011，23（4）：604 -614］