A strain of highly cellulolytic bacteria was obtained from goose gut, and the conditions in enzyme production have been studied in this study. The strain of highly cellulolytic bacterial E2 has been isolated by the Congo red (primary screening) and shaking flask method (secondary screening). The 16S rDNA sequence analysis indicated that strain E2 was Bacillus pumilus sp. On the basis of single factor experiment, the optimal carbon source, nitrogen source, initial pH, fermentation temperature and fermentation time of strain E2 for enzyme production were maizena, beef extract and peptone mixture, 6.0, 42 ℃, and 48 h, respectively.［Chinese Journal of Animal Nutrition，2011，23（3）：466-472］