A Loop-mediated Isothermal Amplification to Detect Genetically Modified Soybean Resistant to Glyphosate and its Processed Products

TIAN Fang1,2，WANG Xiumin2,3*，TENG Da2,3，YANG Yalin2,3，GUAN Qingfeng2,3，AO Changjin1，WANG Jianhua2,3**

doi:10.3969/j.issn.1006-267x.2011.03.019

2011 , Vol. 23 >Issue 03: 480 - 485

DOI: https://doi.org/10.3969/j.issn.1006-267x.2011.03.019

饲料检测

A Loop-mediated Isothermal Amplification to Detect Genetically Modified Soybean Resistant to Glyphosate and its Processed Products

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(1. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China; 2. Key Laboratory of Feed Biotechnology, Ministry of griculture, Beijing 100081, China; 3. Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Online published: 2011-03-20

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Abstract

The study was conducted to set a detection method for exogenous gene of genetically modified (GM) soybean resistant to glyphosate and its processed products by loop-mediated isothermal amplification (LAMP) method. Four specific primers were designed according to the 6 distinct sequences on the target region of CP4-EPSPS gene. Samples of soybean, soybean meal and feedstuff containing soybean were detected by LAMP method. The results indicated that the CP4-EPSPS gene was successfully detected by LAMP method and the sensitivity was 100 to 1 000 fold higher than PCR. The LAMP method is easy to operate and can be used for the rapid identification of GM soybean and its processed products.［Chinese Journal of Animal Nutrition，2011，23（3）：480-485］

Key words： loop mediated isothermal amplification; genetically modified soybean; processed products

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TIAN Fang1,2，WANG Xiumin2,3*，TENG Da2,3，YANG Yalin2,3，GUAN Qingfeng2,3，AO Changjin1，WANG Jianhua2,3** . A Loop-mediated Isothermal Amplification to Detect Genetically Modified Soybean Resistant to Glyphosate and its Processed Products[J]. Chinese Journal of Animal Nutrition, 2011 , 23(03) : 480 -485 . DOI: 10.3969/j.issn.1006-267x.2011.03.019

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