In order to get a synthetic evaluation of the nutritional quality between wild and cultured of Portunus trituberculatus, crude protein, crude lipid, ash, amino acids and fatty acids in muscle, hepatopancreas and ovary of Portunus trituberculatus were determined by adopting national standard method. Results showed that crude protein content in the muscle was the highest, and it was 74.02% for the wild and 81.69% for the culture. Crude fat content in the hepatopancreas was the highest, and crude fat of the wild (57.28%) was 1.4 times than that of the culture (42.42%). There were 38 kinds of fatty acids and the fatty acid composition in hepatopancreas was the most abundant. The contents of saturated fatty acid (SFA) and monounsaturated fatty acid (MUFA) in hepatopancreas were higher than those in ovary and muscle. The total amounts of polyunsaturated fatty acids (PUFA) and EPA+DHA were muscle＞ovary＞hepatopancreas （P<0.05）. The level of EPA+DHA reached up to 20.21%-36.91%, and being more over 2/3 of PUFA. The contents of C18∶2(n-6), C18∶3(n-3) and C20∶4(n-6) in the wild were higher than those in the culture （P<0.05）. The glutamic acid content was the highest in amino acids. Evaluation of amino acids by AAS and CS showed that the lysine relative content in the tissue of wild or culture of Portunus trituberculatus was the richest, the first limiting amino acid was sulfur-containing amino acid (methionine and cystine). The rate of essential amino acids/total amino acids (E/T) and essential amino acids/nonessential amino acids (E/N) were all higher than evaluation criteria of FAO/WHO, and the E/T and E/N in muscle in the wild of Portunus trituberculatus were significantly higher than those in the culture (P<0.05). In conclusion, nutritional composition of Portunus trituberculatus was reasonable, moreover, the quality of wild Portunus trituberculatus was better than that of culture.［Chinese Journal of Animal Nutrition, ２００9，２1（５）:695-702］