The trial was conducted to study the effects of different addition levels of vitamins A and E on their bioavailability in broilers. Nine hundred and sixty 1-day-old AA broilers were divided into four treatments with three replicates in each treatment and eighty broilers in each replicate. Vitamin A levels in the diets of four treatments were 6 375, 8 500, 10 625 and 12 750 IU/kg respectively, while vitamin E levels were 37.5, 50, 62.5 and 75 IU/kg. The trial lasted for 49 days. Other nutrient levels all reached to the recommended standard of AA broilers. The results showed that the daily gain and the ratio of feed to gain could be improved with the increase of vitamin A and E levels. Vitamins A and E in serum and liver could also be improved with the increase of vitamin A and E levels in diet. When vitamin A was decreased from 8 500 IU/kg to  6 375 IU/kg , and vitamin E was from 50 IU/kg to 37.5 IU/kg, vitamin A and E levels in serum were decreased by 8.42%  (P>0.05) and 12.14% （P<0.05）, and vitamin A and E levels in liver were decreased by 9.04% and 5.12% (P>0.05). While vitamin A and E levels were increased to 12 750 and 75 IU/kg, their levels in serum were improved by 6.44% (P>0.05) and 42.21% (P<0.01), which in liver increased by 192.58% and 65.03% (P<0.01) correspondingly. It was concluded that the increase of vitamin A and E levels could improve their levels in serum and liver of broilers.