动物营养学报
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动物营养学报 2017, Vol. 29 Issue (9) :3193-3201    DOI: 10.3969/j.issn.1006-267x.2017.09.022
饲料营养 Feed science and technology 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
α-酮戊二酸和大蒜素对生长猪生长发育及养分表观消化率的影响
刘少娟1,2, 陈家顺1,3, 康保聚3, 蒋谦1,2, 何流琴1,2, 吴飞1,2, 黄牛3, 李欢3, 印遇龙1,2, 姚康1,3
1. 中国科学院亚热带农业生态研究所, 中国科学院亚热带农业生态过程重点实验室, 湖南省畜禽健康养殖工程技术中心, 农业部中南动物营养与饲料科学观测实验站, 长沙 410125;
2. 中国科学院大学, 北京 100049;
3. 湖南农业大学动物科学技术学院, 长沙 410128
Effects of Dietary α-Ketoglutarate and Allicin on Growing Development and Nutrient Apparent Digestibility of Growing Pigs
LIU Shaojuan1,2, CHEN Jiashun1,3, KANG Baoju3, JIANG Qian1,2, HE Liuqin1,2, WU Fei1,2, HUANG Niu3, LI Huan3, YIN Yulong1,2, YAO Kang1,3
1. Key Lab Agro-Ecology Processing Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Hunan Engineering & Research Center of Healthy Breeding Livestock & Poultry, Scientific Observational and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha 410125, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China
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摘要 本试验旨在研究α-酮戊二酸(AKG)和大蒜素对生长猪生长性能、养分表观消化率、血清生化指标以及肠道形态结构的影响。选用40头初始平均体重为(30.64±1.35)kg的健康三元(杜×长×大)杂交生长猪,按体重相近、性别比例相同原则随机分为5组,每组8个重复,每个重复1头猪。5组试验猪分别饲喂无抗基础饲粮(对照组)、无抗基础饲粮+25 mg/kg抗生素(Ⅰ组)、无抗基础饲粮+1% AKG(Ⅱ组)、无抗基础饲粮+0.5%大蒜素(Ⅲ组)、无抗基础饲粮+1% AKG+0.5%大蒜素(Ⅳ组)。预试期7 d,正试期21 d。结果表明:1)与对照组相比,Ⅰ组和Ⅳ组生长猪的平均日增重分别提高了10.80%和9.91%,但差异不显著(P>0.05)。2)与对照组相比,Ⅱ组钙的表观消化率显著提高(P<0.05),Ⅳ组粗蛋白质、钙和磷的表观消化率显著提高(P<0.05),且Ⅳ组较Ⅰ组显著提高了磷的表观消化率(P<0.05)。3)与对照组相比,Ⅱ组血清免疫球蛋白G(IgG)含量显著提高(P<0.05);Ⅲ组血清甘油三酯(TG)含量显著降低(P<0.05);Ⅳ组血清总胆固醇(TCHO)和TG含量显著降低(P<0.05),血清碱性磷酸酶(AKP)、谷草转氨酶(AST)和谷丙转氨酶(ALT)活性及血清免疫球蛋白A(IgA)、IgG含量显著提高(P<0.05)。与Ⅰ组相比,Ⅳ组血清TCHO和TG含量有降低趋势(P>0.05),血清AST和ALT活性显著提高(P<0.05)。4)与对照组相比,Ⅳ组回肠绒毛高度及空肠和回肠的绒毛高度/隐窝深度值显著提高(P<0.05)。5)与对照组相比,Ⅳ组回肠中杯状细胞和淋巴细胞数目显著增加(P<0.05)。综上,无抗饲粮中联合添加1% AKG和0.5%大蒜素可通过改善肠道形态结构,提高对粗蛋白质、钙和磷等养分的消化吸收能力,促进肠道健康,进而改善生长猪的生长性能。
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关键词&alpha   -酮戊二酸   生长猪   大蒜素   生长性能   养分表观消化率   血清生化指标   肠道形态结构     
Abstract: This experiment was conducted to study the effects of dietary α-ketoglutarate (AKG) and allicin on growth performance, nutrient apparent digestibility, serum biochemical parameters and intestinal morphologic structure of growing pigs. Forty healthy crossbred (Duroc×Landrace×Large White) growing pigs with (30.64±1.35) kg were randomly divided into 5 groups with 8 replicates per group and 1 pig per replicate by similar body weight and gender ratio. Pigs in control group were fed a basal diet (without antibiotics), while pigs in the experimental groups were fed the basal diet supplemented with either 25 mg/kg antibiotics (group Ⅰ), or 1.0% AKG (group Ⅱ), or 0.5% allicin (group Ⅲ), or 1.0% AKG combined with 0.5% allicin (group Ⅳ). The experiment lasted for 21 days after 7 days for pre-feeding. The results showed as follows:1) compared with control group, the average daily gain of pigs in groups Ⅰ and Ⅳ was respectively improved by 10.80% and 9.91% without significant difference (P>0.05). 2) Compared with control group, the calcium (Ca) apparent digestibility in group Ⅱ was significantly improved (P<0.05), the apparent digestibility of crude protein (CP), Ca and phosphorus (P) in group Ⅳ was significantly increased (P<0.05). Compared with group Ⅰ, the P apparent digestibility in group Ⅳ was significantly increased (P<0.05). 3) Compared with control group, pigs in group Ⅱ had significantly improved serum immunoglobulin G (IgG) content (P<0.05), and the serum triglyceride content in group Ⅲ was significantly decreased (P<0.05); pigs in group Ⅳ had significantly lower serum contents of total cholesterol (TCHO) and TG (P<0.05), but had significantly higher serum activities of alkaline phosphatase (AKP), glutamic-oxalacetic transaminase (AST) and alanine aminotransferase (ALT) (P<0.05), and significantly higher serum contents of immunoglobulin A (IgA) and IgG (P<0.05). Compared with group Ⅰ, the serum contents of TCHO and TG in group Ⅳ were tend to be decreased (P>0.05), while the serum activities of AST and ALT in group Ⅳ were significantly increased (P<0.05). 4) Compared with control group, the villous height of ileum and the ratio of the villous height to crypt depth of ileum and jejunum in group Ⅳ were significantly improved (P<0.05). 5) Compared with control group, the amounts of goblet cells and lymphocytes in the ileum of group Ⅳ were increased significantly (P<0.05). The results suggest that dietary supplementation with 1.0% AKG and 0.5% allicin can ameliorate intestinal morphologic structure, increase the ability of nutrient digestibility and absorption such as CP, Ca and P, and promote intestinal health, thus improve growth performance of growing pigs.
Keywordsα-ketoglutarate,   growing pigs,   allicin,   growth performance,   nutrient apparent digestibility,   serum biochemical parameters,   intestinal morphologic structure     
收稿日期: 2017-02-16;
基金资助:

中科院百人计划项目(Y451022111);国家自然科学基金面上项目(31472107);湖南省"湖湘青年科技创新人才"项目(2015RS4053);湖南省"湖南省杰出青年基金"项目(2016JJ1015);中国科学院亚热带农业生态过程重点实验室开放基金项目(ISA2016101)

通讯作者 印遇龙,院士,博士生导师,E-mail:yinyulong@isa.ac.cn;姚康,研究员,博士生导师,E-mail:yaokang@isa.ac.cn     Email: yinyulong@isa.ac.cn;yaokang@isa.ac.cn
作者简介: 刘少娟(1991-),女,湖南邵阳人,硕士研究生,从事单胃动物营养与饲料研究。E-mail:2431866181@qq.com
引用本文:   
. α-酮戊二酸和大蒜素对生长猪生长发育及养分表观消化率的影响[J]. 动物营养学报, 2017,V29(9): 3193-3201
. Effects of Dietary α-Ketoglutarate and Allicin on Growing Development and Nutrient Apparent Digestibility of Growing Pigs[J]. Chinese Journal of Animal Nutrition, 2017,V29(9): 3193-3201.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2017.09.022     或     http://www.chinajan.com/CN/Y2017/V29/I9/3193
 
[1] ARDITTI F D,RABINKOV A,MIRON T,et al.Apoptotic killing of B-chronic lymphocytic leukemia tumor cells by allicin generated in situ using a rituximab-alliinase conjugate[J].Molecular Cancer Therapeutics,2005,4(2):325-331.
[2] GARDNER C D,CHATTERJEE L M,CARLSON J J.The effect of a garlic preparation on plasma lipid levels in moderately hypercholesterolemic adults[J].Atherosclerosis,2001,154(1):213-220.
[3] 付大波,王友炜,侯永清,等.α-酮戊二酸对脂多糖刺激断奶仔猪肌肉能量代谢的影响[J].中国农业科学,2011,44(4):814-822.
[4] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激仔猪肠黏膜能量代谢的影响[J].动物营养学报,2009,21(6):892-896.
[5] 刘坚,侯永清,丁斌鹰,等.α-酮戊二酸对脂多糖应激断奶仔猪空肠黏膜蛋白合成和抗氧化能力的影响[J].中国畜牧杂志,2010,46(11):35-38.
[6] 王金泉,项方献,姚刚.中草药结合AKG对断奶仔猪生长及消化吸收功能的影响[J].西北农林科技大学学报:自然科学版,2011,39(5):27-31.
[7] 王友炜.α-酮戊二酸对脂多糖刺激仔猪生长性能、肠道功能和血浆指标的影响[D].硕士学位论文.武汉:武汉工业学院,2010.
[8] 胡泉舟.α-酮戊二酸对断奶仔猪生长性能和肠道功能的影响[D].硕士学位论文.武汉:武汉工业学院,2008.
[9] 刘超良.日粮中添加大蒜素对断奶仔猪生长的影响[D].硕士学位论文.长沙:湖南农业大学,2008.
[10] 张金枝,刘建新,华卫东,等.大蒜和大蒜素对仔猪生产性能和血液生化指标的影响研究[J].家畜生态学报,2008,29(4):56-60.
[11] 余亲平,陈雁群,谢金蝉,等.日粮添加α-酮戊二酸对肉仔鸡生长性能及组织器官发育的影响[J].中国畜牧兽医,2010,37(10):10-14.
[12] 涂兴强.糖萜素、大蒜素在生长育肥猪中的应用研究[D].硕士学位论文.南宁:广西大学,2013.
[13] JONES C,PALMER T E A,GRIFFITHS R.Randomized clinical outcome study of critically ill patients given glutamine-supplemented enteral nutrition[J].Nutrition,1999,15(2):108-115.
[14] 程志斌,葛长荣,苏子峰.大蒜素的研究与应用[J].饲料博览,2001(12):38-40.
[15] 付晓辉,项方献,罗金生,等.中草药结合α-酮戊二酸作为饲料添加剂对断奶仔猪消化吸收功能的影响[J].黑龙江畜牧兽医,2011(7):71-72.
[16] 占今舜,张彬,赵越.大蒜素在动物生产中的应用[J].中国饲料,2012(18):38-41.
[17] 尹清强,李小飞,常娟,等.微生态制剂对哺乳和断奶仔猪生产性能的影响及作用机理研究[J].动物营养学报,2011,23(4):622-630.
[18] 唐晓玲,刘振湘,张石蕊,等.糖萜素对早期断奶仔猪血液生化指标及免疫机能的影响研究[J].湖南环境生物职业技术学院学报,2005,11(3):239-243.
[19] 位莹莹,徐奇友,李晋南,等.不同蛋白质水平饲料中添加α-酮戊二酸对松浦镜鲤生长性能、体成分和血清生化指标的影响[J].动物营养学报,2013,25(12):2958-2965.
[20] 魏玉强,徐奇友,位莹莹,等.不同蛋白源饲料中添加α-酮戊二酸对松浦镜鲤肌肉成分、血清氨基酸和生化指标的影响[J].东北农业大学学报,2015,46(1):94-100.
[21] 刁慧,郑萍,余冰,等.苯甲酸对断奶仔猪生长性能、血清生化指标、养分消化率和空肠食糜消化酶活性的影响[J].动物营养学报,2013,25(4):768-777.
[22] 张庭廷,童希琼,刘锡云.大蒜素降血脂作用及其机理研究[J].中国实验方剂学杂志,2007,13(2):32-35.
[23] 胡新旭,周映华,刘惠知,等.无抗发酵饲料对断奶仔猪生长性能、肠道菌群、血液生化指标和免疫性能的影响[J].动物营养学报,2013,25(12):2989-2997.
[24] 姚浪群,萨仁娜,佟建明,等.安普霉素对仔猪肠道微生物及肠壁组织结构的影响[J].畜牧兽医学报,2003,34(3):250-257.
[25] 陈迪,王连生,徐奇友.α-酮戊二酸对杂交鲟肠道形态、消化酶活力和抗氧化能力的影响[J].大连海洋大学学报,2015,35(4):363-368.
[26] 王蕾,吴信,付大波.α-酮戊二酸对动物肠道黏膜作用的研究进展[J].黑龙江畜牧兽医,2010(11):36-37.
[27] 石玉祥,闫金坤,王雪敏,等.中药提取物对应激小鼠肠道上皮内淋巴细胞和杯状细胞数量及IL-2水平的影响[J].中国兽医科学,2011,41(1):85-88.
[28] 董文逍,曹海龙,王邦茂.杯状细胞在肠道疾病发病中作用的研究进展[J].国际消化病杂志,2015,35(4):244-246,269.
[29] XU R J,WANG F,ZHANG S H.Postnatal adaptation of the gastrointestinal tract in neonatal pigs:a possible role of milk-borne growth factors[J].Livestock Production Science,2000,66(2):95-107.
[30] 张玉仙,王文利,陈耀星,等.大蒜溶液对小鼠小肠黏膜结构、上皮内淋巴细胞和杯状细胞的影响[J].北京农业职业学院学报,2008,22(1):33-37.
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