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动物营养学报 2017, Vol. 29 Issue (9) :3211-3219    DOI: 10.3969/j.issn.1006-267x.2017.09.024
饲料营养 Feed science and technology 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
大菱鲆幼鱼对玉米蛋白粉中营养物质的表观消化率及添加胆汁酸和酶制剂对其产生的影响
田莹, 何艮, 周慧慧, 王旋, 麦康森
中国海洋大学水产学院, 农业部水产动物营养与饲料重点实验室, 青岛 266003
Nutrient Apparent Digestibility Coefficients of Corn Gluten Meal for Juvenile Turbot (Scophthalmus maximus L.) and Effects of Adding Bile Acid and Enzyme Preparation on Them
TIAN Ying, HE Gen, ZHOU Huihui, WANG Xuan, MAI Kangsen
The Key Laboratory of Aquaculture Nutrition and Feed, Ministry of Agriculture, Ocean University of China, Qingdao 266003, China
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摘要 本试验旨在研究大菱鲆幼鱼对玉米蛋白粉中干物质、粗蛋白质、氨基酸和总能的表观消化率,以及在玉米蛋白粉中分别添加胆汁酸(纯度≥30%)和酶制剂[中性蛋白酶(1×105 U/g)和木瓜蛋白酶(4×105 U/g)组成的复合酶,二者活性比为2:1]对其营养物质表观消化率的影响。首先配制基础饲料,试验饲料由70%的基础饲料和30%的待测蛋白质源[未进行任何处理的玉米蛋白粉(CGM)、添加胆汁酸的玉米蛋白粉(CGMB)和添加酶制剂的玉米蛋白粉(CGME)]组成。所有饲料均添加0.1%的三氧化二钇(Y2O3)为外源指示剂。将初始体重为(13.00±0.01)g大菱鲆幼鱼随机分为4组,每组3个重复,每个重复40尾鱼,分别投喂相应饲料2周后采用后肠挤压法收集粪便。养殖周期为10周。结果表明:3种不同处理形式的玉米蛋白粉中干物质、粗蛋白质、总氨基酸、总能的表观消化率分别为25.99%~43.34%、48.62%~60.72%、48.41%~67.67%、35.07%~52.34%,且总氨基酸与粗蛋白质表观消化率的变化趋势一致。其中,大菱鲆幼鱼对CGMB中各营养物质的表观消化率均最高,显著高于CGM和CGMEP<0.05),且CGMB中干物质、粗蛋白质、总氨基酸、总能的表观消化率较CGM分别提高了17.35%、12.10%、19.26%、17.27%;大菱鲆幼鱼对CGME中各营养物质的表观消化率也得到改善,其干物质、粗蛋白质、氨基酸、总能的表观消化率较CGM分别提高了9.53%、4.37%、4.29%、12.25%,效果显著(P<0.05)。由结果可知,添加胆汁酸和酶制剂均能够提高大菱鲆幼鱼对玉米蛋白粉中营养物质的表观消化率。
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关键词大菱鲆幼鱼   玉米蛋白粉   胆汁酸   酶制剂   表观消化率     
Abstract: This experiment was conducted to determine the apparent digestibility coefficients (ADCs) of dry matter (DM), crude protein (CP), amino acid (AA) and gross energy (GE) of corn gluten meal, and the effects of adding bile acid (purity ≥ 30%) and enzyme preparation[compound enzyme which was composed of neutral protease (1×105 U/g) and papain (4×105 U/g), and the activity ratio of them was 2:1] on ADCs of nutrients in corn gluten meal. A basal diet was prepared firstly, and then the test diets were prepared which contained 70% basal diet and 30% test feed ingredients[untreated corn gluten meal (CGM), corn gluten meal supplemented with bile acid (CGMB), and corn gluten meal supplemented with enzyme preparation (CGME)]. All diets were added 0.1% yttrium trioxide as an exogenous indicator. Juvenile turbot with the average body weight of (13.00±0.01) g were randomly divided into 4 groups with 3 replicates in each group and 40 fish in each replicate. The fish in different group were fed with the corresponding diet, and fecal samples were collected with the method of squeezing the hindgut after 2 weeks. The feeding period was 10 weeks. The results showed that the ADCs of DM, CP, total AA and GE of three protein sources were 25.99% to 43.34%, 48.62% to 60.72%, 48.41% to 67.67%, and 35.07% to 52.34%, respectively. The ADC of total AA of each protein source presented the similar tendency with the ADC of CP. CGMB showed the highest ADCs of all nutrients for juvenile turbot, which were significantly higher than those of CGM and CGME (P<0.05), and the ADCs of DM, CP, total AA and GE of CGMB were increased by 17.35%, 12.10%, 19.26% and 17.27%, respectively, compared with CGM; the ADCs of all nutrients of CGME for juvenile turbot were also improved, the ADCs of DM, CP, total AA and GE of CGMB were increased by 9.53%, 4.37%, 4.29% and 12.25%, respectively, compared with CGM, and the effects were significant (P<0.05). The results indicate that adding bile acid and enzyme preparation all contribute to the improvement of ADCs of nutrients of corn gluten meal for juvenile turbot.
Keywordsjuvenile turbot,   corn gluten meal,   bile acid,   enzyme preparation,   apparent digestibility coefficient     
收稿日期: 2017-03-02;
基金资助:

公益性行业专项(201303053)——替代鱼用饲料中鱼粉的新蛋白源开发利用技术

通讯作者 何艮,教授,博士生导师,E-mail:hegen@ouc.edu.cn     Email: hegen@ouc.edu.cn
作者简介: 田莹(1991-),女,河南新乡人,硕士研究生,从事水产动物营养与饲料学研究。E-mail:15527262478@163.com
引用本文:   
. 大菱鲆幼鱼对玉米蛋白粉中营养物质的表观消化率及添加胆汁酸和酶制剂对其产生的影响[J]. 动物营养学报, 2017,V29(9): 3211-3219
. Nutrient Apparent Digestibility Coefficients of Corn Gluten Meal for Juvenile Turbot (Scophthalmus maximus L.) and Effects of Adding Bile Acid and Enzyme Preparation on Them[J]. Chinese Journal of Animal Nutrition, 2017,V29(9): 3211-3219.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2017.09.024     或     http://www.chinajan.com/CN/Y2017/V29/I9/3211
 
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