动物营养学报
         首页        期刊介绍        编委会         编辑部         投稿须知        英文刊IFA        会议信息        联系我们      留言与回复
动物营养学报 2018, Vol. 30 Issue (9) :3377-3387    DOI: 10.3969/j.issn.1006-267x.2018.09.005
磷营养与饲料专栏 Special section:phosphorus nutrition and feed 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
大口黑鲈对磷的最适需求量以及高水溶性磷酸一二钙的相对生物学利用率研究
时博1, 郁欢欢1, 郑银桦1, 吴秀峰1, 陈沛1, 梁旭方2, 薛敏1,3
1. 中国农业科学院饲料研究所, 国家水产饲料安全评价基地, 北京 100081;
2. 华中农业大学水产学院, 武汉 430070;
3. 农业部饲料生物技术重点开放实验室, 北京 100081
Study on Optimum Requirement for Phosphorus and Relative Biological Availability of High Soluble Monodicalcium Phosphate of Largemouth Bass (Micropterus salmoides)
SHI Bo1, YU Huanhuan1, ZHENG Yinhua1, WU Xiufeng1, CHEN Pei1, LIANG Xufang2, XUE Min1,3
1. National Aquafeed Safety Assessment Station, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
2. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
3. Key Laboratory of Feed Biotechnology of Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Download: PDF (1464KB)   HTML (1KB)   Export: BibTeX or EndNote (RIS)      Supporting Info
摘要 本试验在低磷基础饲料中添加不同水平的磷酸二钙(MCP)和高水溶性磷酸一二钙(MDCP),通过研究其对大口黑鲈生长性能、氮和磷的消化率与沉积率以及全鱼和椎骨磷含量与全鱼粗灰分含量的影响,确定大口黑鲈对磷的最适需求量以及高水溶性MDCP相对于MCP的生物学利用率。首先配制低磷基础饲料,然后在低磷基础饲料基础上分别添加8.86、11.08、13.29、15.51 g/kg MCP,以及9.34、11.67、14.01、16.34 g/kg高水溶性MDCP,使得2种磷酸盐所提供的磷均分别为2.0、2.5、3.0、3.5 g/kg,9种试验饲料分别命名为P0、P2、P2.5、P3、P3.5、DP2、DP2.5、DP3、DP3.5。每种饲料投喂4桶(重复)初始体重为(8.53±0.01)g的大口黑鲈,每个桶放养40尾,养殖周期为8周。结果显示:饲料中添加不同水平的MCP和高水溶性MDCP均显著提高了大口黑鲈的增重率、磷消化率与沉积率和全鱼粗灰分含量(P<0.05),且2种磷源对以上指标的影响差异不显著(P>0.05)。随MCP添加水平的升高,大口黑鲈的增重率和全鱼粗灰分含量均呈先上升后稳定的趋势,磷沉积率有先上升后降低的趋势,均以P0组最低。随高水溶性MDCP添加水平的升高,大口黑鲈的增重率和磷消化率呈现持续上升的趋势,磷沉积率有先上升后降低的趋势,以P0组磷沉积率最低,DP3组磷沉积率最高。分别以增重率和磷沉积率为指标,通过斜率比法计算得出高水溶性MDCP相对于MCP的生物学利用率分别为100%和118%。以MCP为磷源,饲料可消化磷含量为0.51%时,增重率和磷沉积率达到最高。以高水溶性MDCP为磷源,饲料可消化磷含量为0.52%时,磷沉积率达到最高。
Service
把本文推荐给朋友
加入我的书架
加入引用管理器
Email Alert
RSS
作者相关文章
关键词大口黑鲈   磷酸二氢钙   高水溶性磷酸一二钙   磷需求量   生物学利用率     
Abstract: In this experiment, various levels of monocalcium phosphate (MCP) and high soluble monodicalcium phosphate (MDCP) were added to low-phosphorus basal diet, in order to study the effects of them on growth performance, apparent digestibility coefficients of phosphorus (ADCp) and nitrogen (ADCn), retention ratios of phosphorus (PRR) and nitrogen value (NRR), phosphorus content of vertebra and whole body, and ash content of whole body, and to determine the optimum requirement for phosphorus and the biological availability of high soluble MDCP relative to MCP of largemouth bass (Micropterus salmoides). A low-phosphorus basal diet was prepared firstly, and then 8.86, 11.08, 13.29 and 15.51 g/kg of MCP, and 9.34, 11.67, 14.01 and 16.34 g/kg of high soluble MDCP were added into the low-phosphorus basal diet to equally supply 2.0, 2.5, 3.0 and 3.5 g/kg of phosphorus, respectively. The nine experimental diets were accordingly named P0, P2, P2.5, P3.0, P3.5 and DP2.0, DP2.5, DP3.0, DP3.5. Each diet was fed to four replicates (tanks) with 40 largemouth bass[initial body weight:(8.53±0.01) g] in each tank for 8 weeks. The results showed as follows:diets supplemented with different levels of MCP and high soluble MDCP both significantly increased the weight gain rate (WGR), ADCp, PRR and whole body ash content of the largemouth bass (P<0.05), but there were no significant differences in them between the two phosphorus sources (P>0.05). With the MCP supplemental level increasing, the WGR and whole body ash content were increased firstly and then went in a stabilization trend, the PRR was increased firstly and then declined, and all of them in P0 group were the lowest. With the high soluble MDCP supplemental level increasing, the WGR and ADCp showed a upward tendency, the PRR was increased firstly and then decreased, and the lowest and the highest values were observed in P0 and DP3 groups, respectively (P<0.05). The biological availability of high soluble MDCP relative to MCP is 100% and 118% based on the WGR and PRR through slope method, respectively. With MCP as the source of phosphorus, the WGR and PRR reach the highest when dietary digestible phosphorus content is 0.51%. With high soluble MDCP as the source of phosphorus, the PRR reached the highest when dietary digestible phosphorus content is 0.52%.
Keywordslargemouth bass (Micropterus salmoides),   monocalcium phosphate,   high soluble monodicalcium phosphate,   phosphorus requirement,   relative biological availability     
收稿日期: 2017-11-20;
基金资助:

国家重点研发计划项目(2016YFF0201800);国家自然科学基金项目(31372539,31572631);国家重点基础研究发展计划项目(2014CB138600);北京市现代农业产业技术体系(SCGWZJ 20171103-1);中国农业科学院基本科研业务费(1610382016010);云天化国际植物营养研究院开放项目(YTHZWYJY2016004)

通讯作者 薛敏,研究员,博士生导师,E-mail:xuemin@cass.cn     Email: xuemin@cass.cn
作者简介: 时博(1992-),女,辽宁阜新人,硕士研究生,研究方向为水产动物营养与饲料科学。E-mail:415670966@qq.com
引用本文:   
. 大口黑鲈对磷的最适需求量以及高水溶性磷酸一二钙的相对生物学利用率研究[J]. 动物营养学报, 2018,V30(9): 3377-3387
. Study on Optimum Requirement for Phosphorus and Relative Biological Availability of High Soluble Monodicalcium Phosphate of Largemouth Bass (Micropterus salmoides)[J]. Chinese Journal of Animal Nutrition, 2018,V30(9): 3377-3387.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2018.09.005     或     http://www.chinajan.com/CN/Y2018/V30/I9/3377
 
[1] 李二超,陈立侨.大口黑鲈的营养需要研究进展[J].现代农业科技,2011(21):312-316,318.
[2] 孙宏君,刘冰,曲强.钙、磷对动物的重要影响[J].畜牧兽医科技信息,2009(9):18.
[3] 萧培珍,张宝彤,唐精,等.鱼类磷营养需求及原料消化率研究进展[J].中国饲料,2009(2):34-37.
[4] YUAN Y C,YANG H J,GONG S Y,et al.Dietary phosphorus requirement of juvenile Chinese sucker,Myxocyprinus asiaticus[J].Aquaculture Nutrition,2015,17(2):159-169.
[5] SHAO Q J,MA J J,XU Z R,et al.Dietary phosphorus requirement of juvenile black seabream,Sparus macrocephalus[J].Aquaculture,2008,277(1/2):92-100.
[6] ROY P K,LALL S P.Dietary phosphorus requirement of juvenile haddock (Melanogrammus aeglefinus L.)[J].Aquaculture,2003,221(1/2/3/4):451-468.
[7] RODEHUTSCORD M,GREGUS Z,PFEFFER E.Effect of phosphorus intake on faecal and non-faecal phosphorus excretion in rainbow trout (Oncorhynchus mykiss) and the consequences for comparative phosphorus availability studies[J].Aquaculture,2000,188(3/4):383-398.
[8] JOKINEN E I,VIELMA J,AALTONEN T M,et al.The effect of dietary phosphorus deficiency on the immune responses of European whitefish (Coregonus lavaretus L.)[J].Fish & Shellfish Immunology,2003,15(2):159-168.
[9] 姚鹰飞.吉富罗非鱼幼鱼和成鱼饲料有效磷及钙磷比的研究[D].硕士学位论文.武汉:华中农业大学,2012.
[10] 郑秋珊.西伯利亚幼鲟(Acipenser baeri)磷需要量的研究[D].硕士学位论文.哈尔滨:东北农业大学,2009.
[11] 朱琳.大菱鲆和牙鲆无机盐锌、磷的营养生理研究[D].硕士学位论文.青岛:中国海洋大学,2011.
[12] CHENG K M,HU C Q,LIU Y N,et al.Effects of dietary calcium,phosphorus and calcium/phosphorus ratio on the growth and tissue mineralization of Litopenaeus vannamei reared in low-salinity water[J].Aquaculture,2006,251(2/3/4):472-483.
[13] 刘仙钦.军曹鱼(Rachycentron canadum)幼鱼饲料中适宜磷源、磷水平以及钙磷比研究[D].硕士学位论文.广州:广东海洋大学,2010.
[14] 秦巍仑,杨毅,冷向军,等.不同磷酸二氢钙含量饲料中添加植酸酶对建鲤生长、磷利用、体组成和消化酶活性的影响[J].上海海洋大学学报,2015,24(3):383-390.
[15] ZHANG C X,MAI K S,AI Q H,et al.Dietary phosphorus requirement of juvenile Japanese seabass,Lateolabrax japonicus[J].Aquaculture,2006,255(1/2/3/4):201-209.
[16] ZHANG C X,AI Q H,MAI K S,et al.Dietary lysine requirement of large yellow croaker,Pseudosciaena crocea R[J].Aquaculture,2006,253(1/2/3/4):123-127.
[17] PIMENTEL-RODRIGUES A M,OLIVA-TELES A.Phosphorus requirements of gilthead sea bream (Sparus aurata L.) juveniles[J].Aquaculture Research,2015,32(Suppl.1):157-161.
[18] CHAVEZ-SANCHEZ C,MARTINEZ-PALACIOS C A,MARTINEZ-PEREZ G,et al.Phosphorus and calcium requirements in the diet of the American cichlid Cichlasoma urophthalmus (Günther)[J].Aquaculture Nutrition,2015,6(1):1-9.
[19] 熊文.磷源和磷浓度对黑鲷幼鱼生长性能的影响[D].硕士学位论文.杭州:浙江大学,2010.
[20] SKONBERG D I,YOGEV L,HARDY R W,et al.Metabolic response to dietary phosphorus intake in rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,1997,157(1/2):11-24.
[21] BUREAU D P,CHO C Y.Phosphorus utilization by rainbow trout (Oncorhynchus mykiss):estimation of dissolved phosphorus waste output[J].Aquaculture,1999,179(1/2/3/4):127-140.
[22] 郑秋珊,张文璐,徐奇友,等.饲料中不同水平磷对西伯利亚幼鲟(Acipenser baeri)血浆生化指标的影响[J].水产学杂志,2009,22(1):38-41.
[23] 胡王龙.饲料磷对黑鲷幼鱼生长和组织生化指标的影响[D].硕士学位论文.杭州:浙江大学,2005.
[24] 陈建明,叶金云,潘茜,等.翘嘴鲌鱼种对磷的需求量[J].水生生物学报,2007,31(1):99-103.
[25] 陈冰,潘庆,郑卫川,等.不同磷源对奥尼罗非鱼幼鱼生长性能的影响[J].饲料工业,2007,28(10):26-28.
[26] 赵朝阳,周洪琪,陈建明,等.花对饲料中磷的营养需求[J].水产学报,2008,32(4):614-620.
[27] 杨雨虹,郭庆,陈松波,等.鲤鱼对不同磷源磷生物学利用率的研究[J].东北农业大学学报,2006,37(4):484-488.
[28] 钟观运.不同磷源对黑鲷幼鱼生长性能的影响[D].硕士学位论文.杭州:浙江大学,2009.
[29] 文华,严安生,高强,等.史氏鲟幼鲟对饲料中磷的需要量[J].水产学报,2008,32(2):242-248.
[30] 蒋明,姚鹰飞,文华,等.吉富罗非鱼成鱼对饲料中有效磷的需要量[J].水产学报,2013,37(11):1725-1732.
[31] 林小勇.花鲈幼鱼饲料磷需要量研究[D].硕士学位论文.杭州:浙江大学,2007.
[1] 万敏艳, 张保海, 王宏博, 唐德富.磷酸一二钙对肉仔鸡相对生物学利用率的研究[J]. 动物营养学报, 2018,30(9): 3353-3363
[2] 吕小康, 柴建民, 王杰, 司丙文, 崔凯, 王世琴, 屠焰, 刁其玉, 张乃锋.饲用高水溶性磷酸一二钙对断奶仔猪生长性能、饲粮消化率、血清和骨骼指标的影响[J]. 动物营养学报, 2018,30(9): 3337-3352
[3] 李文静, 常文环, 陈志敏, 郑爱娟, 刘国华.磷酸一二钙对肉鸭相对生物学利用率的研究[J]. 动物营养学报, 2018,30(9): 3364-3376
[4] 时博, 郁欢欢, 梁晓芳, 陈沛, 陈雪松, 卢存仁, 郑银桦, 吴秀峰, 梁旭方, 薛敏.酵母水解物对大口黑鲈生长性能、血浆生化指标以及肝脏组织健康的影响[J]. 动物营养学报, 2018,30(7): 2772-2781
[5] 王双, 罗莉, 石亚庆, 唐毅, 李战福, 李伟龙, 罗浩.湘云鲫饲料中磷酸二氢钙的适宜添加量[J]. 动物营养学报, 2018,30(10): 3985-3992
[6] 朱婷婷, 金敏, 孙蓬, 李晨晨, 马红娜, 罗嘉翔, 袁野, 周歧存.饲料脂肪水平对大口黑鲈形体指标、组织脂肪酸组成、血清生化指标及肝脏抗氧化性能的影响[J]. 动物营养学报, 2018,30(1): 126-137
[7] 李宁, 郑银桦, 吴秀峰, 王鑫, 薛敏, 吴立新.大口黑鲈对饲料中酵母硒的耐受性研究[J]. 动物营养学报, 2017,29(6): 1949-1960
[8] 李志斐, 龚望宝, 王金林, 王广军, 余德光, 谢骏.冰鲜杂鱼和人工配合饲料对大口黑鲈肌肉品质及健康状况影响的评价[J]. 动物营养学报, 2017,29(11): 4180-4188
[9] 连雪原, 陈乃松, 王孟乐, 闫春为, 丁桂涛.大口黑鲈维生素A需求量[J]. 动物营养学报, 2017,29(10): 3819-3830
[10] 刘正群, 张祖翔, 陈亮, 刘静波, 张宏福.生长猪基础饲粮组成对磷酸氢钙和磷酸二氢钙中磷的全肠道真消化率的影响[J]. 动物营养学报, 2016,28(8): 2542-2550
[11] 曾晓霭, 徐树德, 陈清华, 轩岩俊, 赵青海.中性植酸酶部分替代磷酸二氢钙对异育银鲫生长性能、体成分及磷利用率的影响[J]. 动物营养学报, 2016,28(6): 1710-1719
[12] 于利莉, 薛敏, 王嘉, 韩芳, 郑银桦, 吴秀峰, 吴立新.大口黑鲈对饲料中丁基羟基茴香醚的耐受性评价[J]. 动物营养学报, 2016,28(3): 747-758
[13] 郑银桦, 彭聪, 吴秀峰, 韩芳, 薛敏, 王嘉, 胡亮.酵母酶解物对大口黑鲈生长性能、脂类代谢及肠道组织结构的影响[J]. 动物营养学报, 2015,27(5): 1605-1612
[14] 刘金桃, 艾立川, 王嘉, 韩芳, 薛敏, 郑银桦, 吴秀峰, 张彦娇.大口黑鲈对饲料中乙氧基喹啉的耐受性评价[J]. 动物营养学报, 2015,27(4): 1152-1162
[15] 吴学壮, 张铁涛, 于淼, 刘志, 郭俊刚, 高秀华, 杨福合, 邢秀梅.育成期水貂不同铜源相对生物学利用率和血清生化指标的研究[J]. 动物营养学报, 2015,27(2): 485-494
Copyright 2010 by 动物营养学报