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动物营养学报 2018, Vol. 30 Issue (9) :3567-3580    DOI: 10.3969/j.issn.1006-267x.2018.09.027
水产营养 Aquaculture Nutrition 最新目录 | 下期目录 | 过刊浏览 | 高级检索 << Previous Articles | Next Articles >>
饲料花生四烯酸水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响
王成强1, 王际英1, 黄炳山1, 李宝山1, 孙永智1, 王晓艳1, 马长兴2
1. 山东省海洋资源与环境研究院, 山东省海洋生态修复重点实验室, 烟台 264006;
2. 上海海洋大学水产与生命学院, 上海 201306
Effects of Dietary Arachidonic Acid Level on Growth Performance, Antioxidant Ability, Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)
WANG Chengqiang1, WANG Jiying1, HUANG Bingshan1, LI Baoshan1, SUN Yongzhi1, WANG Xiaoyan1, MA Changxing2
1. Shandong Provincial Key Laboratory of Restoration for Marine Ecology, Shandong Marine Resource and Environment Research Institute, Yantai 264006, China;
2. College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China
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摘要 本试验旨在研究饲料花生四烯酸(ARA)水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响。通过在基础饲料中添加ARA纯化油,制成ARA水平分别为0.04%、0.17%、0.35%、0.66%、1.29%和2.16%(干物质基础)的等氮等脂的试验饲料(分别命名为S1、S2、S3、S4、S5和S6)。将上述饲料投喂初始体重为(23.77±0.98)g的珍珠龙胆石斑鱼幼鱼,每种饲料设3个重复,每个重复投喂30尾鱼,试验期为8周。结果表明:1)特定生长率(SGR)和饲料效率(FE)随饲料ARA水平的升高均呈先升高后降低的趋势,且SGR和FE均在S4组有最大值,均显著高于S1组(P<0.05)。全鱼和肝脏粗脂肪含量均在S3组最低,显著低于S5和S6组(P<0.05)。2)S4组肝脏超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和总抗氧化能力(T-AOC)与S3组差异不显著(P>0.05),但显著高于S1与S6组(P<0.05)。S3与S4组肝脏丙二醛(MDA)含量显著低于S1和S6组(P<0.05)。3)血清中谷草转氨酶(AST)、谷丙转氨酶(ALT)和碱性磷酸酶(AKP)活性均在S4组最低,且显著低于S1与S6组(P<0.05)。4)肝脏和肌肉中C20:3n-6和C20:4n-6含量随饲料ARA水平的提高而显著升高(P<0.05),而C18:3n-6、C20:5n-3和C22:6n-3含量则随之有不同程度的下降。由此得出,饲料中适宜水平(0.35%~0.66%)的ARA能够促进珍珠龙胆石斑鱼幼鱼的生长,提高抗氧化能力与肝脏健康水平,以SGR与FE作为评价指标,经折线模型回归分析得出珍珠龙胆石斑鱼幼鱼饲料中ARA的适宜水平分别为饲料干重的0.45%和0.56%。
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关键词珍珠龙胆石斑鱼   花生四烯酸   生长性能   血清生化指标   脂肪酸组成     
Abstract: An 8-week feeding experiment was conducted to evaluate the effects of dietary arachidonic acid (ARA) level on growth performance, antioxidant ability, serum biochemical parameters and fatty acid compo sition in liver and muscle of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀×Epinephelus lanceolatus♂). Six isonitrogenous and isoenergetic diets were formulated with the ARA level of 0.04%, 0.17%, 0.35%, 0.66%, 1.29% and 2.16% (dry matter basis) (named S1, S2, S3, S4, S5 and S6, separately), respectively. Each diet had 3 replicates and each replicate cultured 30 juvenile hybrid grouper with an initial body weight of (23.77±0.98) g. The results showed as follows:1) the specific growth rate (SGR) and feed efficiency (FE) increased at first, and then decreased with the increase of dietary ARA level, which reaching their peaks at S4 group (P<0.05), and significantly higher than S1 group (P<0.05). The body and liver ether extract content of S3 group was the lowest, and significantly lower than that of S5 and S6 groups (P<0.05). 2) The activities of superoxide dismutase (SOD) and catalase (CAT), total antioxidant capacity (T-AOC) in liver of S4 group were no significant differences with S3 group (P>0.05), but significantly higher than those of S1 and S6 groups (P<0.05). The malondialdehyde (MDA) content in liver of S3 and S4 groups was significantly lower than that of S1 and S6 groups (P<0.05). 3) The lowest values of the activities of aspartate aminotransferase (AST), cereal third transaminase (ALT) and alkaline phosphatase (AKP) in serum were found in S4 group, and significantly lower than those of S1 and S6 groups (P<0.05). 4) The contents of C20:3n-6 and C20:4n-6 in liver and muscle were significantly increased with the increase of dietary ARA level, but the contents of C18:3n-6, C20:5n-3 and C22:6n-3 were decreased with different extents. These results indicate that optimal dietary ARA level (0.35% to 0.66%) can improve the growth of juvenile hybrid grouper, and increase the antioxidant ability and liver health level. The broken-line model analysis based on SGR and FE indicate that the optimal level of dietary ARA is 0.45% and 0.56% of diet on dry matter basis for juvenile hybrid grouper, respectively.
KeywordsEpinephelus fuscoguttatus♀×Epinephelus lanceolatus,   arachidonic acid,   growth performance,   serum biochemical parameters,   fatty acid composition     
收稿日期: 2018-03-07;
基金资助:

山东省重点研发计划(2016GSF115005);山东省科学自然基金(ZR2015CQ023);烟台市科技发展计划(2016ZH068);国家海洋公益性行业科研专项(201505022)

通讯作者 王际英,研究员,硕士生导师,E-mail:ytwjy@126.com     Email: ytwjy@126.com
作者简介: 王成强(1988-),男,山东潍坊人,研究实习员,硕士,研究方向为水产动物营养。E-mail:chengqiangwang@126.com
引用本文:   
. 饲料花生四烯酸水平对珍珠龙胆石斑鱼幼鱼生长性能、抗氧化能力、血清生化指标以及肝脏和肌肉脂肪酸组成的影响[J]. 动物营养学报, 2018,V30(9): 3567-3580
. Effects of Dietary Arachidonic Acid Level on Growth Performance, Antioxidant Ability, Serum Biochemical Parameters and Fatty Acid Composition in Liver and Muscle of Juvenile Hybrid Grouper (Epinephelus fuscoguttatus♀×Epinephelus lanceolatus♂)[J]. Chinese Journal of Animal Nutrition, 2018,V30(9): 3567-3580.
链接本文:  
http://www.chinajan.com/CN/10.3969/j.issn.1006-267x.2018.09.027     或     http://www.chinajan.com/CN/Y2018/V30/I9/3567
 
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