Effects of Bacillus subtilis on Growth Performance and Intestinal Health of Broilers Challenged by Clostridium perfringens
HU Jun1, ZHANG Keying1, BAI Shiping1, WANG Jianping1, ZENG Qiufeng1, PENG Huanwei1, LUAN Chao2, DING Xuemei1
1. Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;
2. Adisseo Life Science(Shanghai) Co., Ltd., Shanghai 201204, China
Abstract:The purpose of this study was to investigate the effects of Bacillus subtilis in the diet on the growth performance and intestinal health of broilers challenged by Clostridium perfringens. A total of 480 1-day-old male broilers (Ross 308) were randomly assigned to 5 groups with 8 replicates per group and 12 broilers per replicate. The 5 groups were control group (CON group), Clostridium perfringens challenge group (CP group), CP+10 mg/kg enramycin group (ENRA group), CP+10 mg/kg Bacillus subtilis (contained 1010 CFU/g) group (BS group) and CP+5 mg/kg enramycin+10 mg/kg Bacillus subtilis group (ENRA+BS group). All the broilers in the challenge group were orally administered with a mixed Eimeria suspension (11 000 spores/mL per broiler) at day 14, and a suspension of Clostridium perfringens (108 CFU/mL per broiler) was orally administered at days 16 to 18. The trial lasted for 42 days. The results showed as follows: 1) compared with CP group, feed/gain (F/G) of broilers at days 1 to 21 in ENRA group, BS group and ENRA+BS group had a tendency to decrease (P=0.051). Compared with CON group, F/G of broilers at days 1 to 42 in ENRA group and ENRA+BS group significantly reduced (P<0.05). 2) Compared with the CP group, the jejunal lesion score of broilers at day 28 in ENRA group, BS group and ENRA+BS group significantly reduced (P<0.01). 3) At days 21 and 42, the crypt depth in the ENRA group was significantly lower than that in the CP group, BS group and ENRA+BS group (P<0.01). At day 42, compared with the CP group, the villus height/crypt depth (VH/CD) in ENRA group significantly increased (P<0.05), and the VH/CD in the BS group and ENRA+BS group increased, but the difference was not significant (P>0.05). 4) At day 21, compared with CP group, secretory immunoglobulin A (SIgA) content in jejunal mucosa in ENRA+BS group significantly increased (P<0.05); at day 28, compared with CP group, immunoglobulin G (IgG) in jejunal mucosa in BS group and ENRA+BS group significantly increased (P<0.05). 5) Compared with the CP group, the number of Escherichia coli at day 21 in ENRA group, BS group and ENRA+BS group significantly reduced (P<0.01), and the number of Clostridium perfringens at day 28 in ENRA group, BS group and ENRA+BS group significantly reduced (P<0.01). It was concluded that Bacillus subtilis can improve the intestinal health and growth performance of broilers to a certain extent. When combined with enramycin (5 mg/kg), the effect is better, thus reducing the use of antibiotics.
胡均, 张克英, 白世平, 王建萍, 曾秋凤, 彭焕伟, 栾超, 丁雪梅. 枯草芽孢杆菌对产气荚膜梭菌攻毒肉鸡生长性能和肠道健康的影响[J]. 动物营养学报, 2019, 31(5): 2127-2135.
HU Jun, ZHANG Keying, BAI Shiping, WANG Jianping, ZENG Qiufeng, PENG Huanwei, LUAN Chao, DING Xuemei. Effects of Bacillus subtilis on Growth Performance and Intestinal Health of Broilers Challenged by Clostridium perfringens. Chinese Journal of Animal Nutrition, 2019, 31(5): 2127-2135.
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