Abstract：This experiment was conducted to study the effects of dietary rape bee pollen on performance, egg quality and serum biochemical indexes of Luhua hens during peak period of laying and in later laying period. In experiment 1, three hundred and eight-four Luhua hens with 32-week-old (during peak period of laying) and about 70% laying rate were randomly divided into 4 groups with 4 replicates per group and 24 hens per replicate. The test lasted for 8 weeks. In experiment 2, sixty Luhua hens with 64-week-old (in later laying period) and about 50% laying rate were randomly divided into 4 groups with 5 replicates per group and 3 hens per replicate. The test lasted for 6 weeks. Hens in control group were fed a basal diet, and the others in experimental group were fed the basal diets supplemented with 0.5%, 1.0% and 1.5% rape bee pollen, respectively. Laying performance and egg quality were measured during experiments, and serum biochemical indexes were tested at the end of experiments. The results showed as follows:compared with the control group, 1) dietary 1.0% rape bee pollen significantly increased laying rate of Luhua hens in later laying period (P<0.05). 2) Dietary 1.5% rape bee pollen significantly increased the value of eggshell yellow (b*) in the 4th week and yolk color in the 8th week of Luhua hens during peak period of laying (P<0.05). 3) Dietary 0.5% and 1.0% rape bee pollen significantly decreased the content of triglyceride in serum of Luhua hens during peak period of laying (P<0.05), and dietary 1.5% rape bee pollen significantly decreased the content of total cholesterol in serum (P<0.05). 4) Dietary 1.0% rape bee pollen significantly increased the activity of total super oxide dismutase in serum and significantly decreased the content of malondialdehyde in serum of Luhua hens during peak period of laying (P<0.05), and dietary 1.5% rape bee pollen significantly increased the total antioxidant capacity in serum and significantly decreased the content of malondialdehyde in serum (P<0.05). 5) Dietary 0.5% and 1.5% rape bee pollen significantly increased the content of immunoglobulin G in serum of Luhua hens in later laying period (P<0.05), and dietary 1.0% rape bee pollen significantly increased the content of immunoglobulin A in serum (P<0.05). Therefore, dietary rape bee pollen can improve performance and egg quality, decrease blood lipids and enhance antioxidant capacity and immune function of Luhua hens, and the suitable supplemental level of rape bee pollen in diet is 0.80% to 0.95%.
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