Abstract:The aim of this study was to investigate the effects of Bacillus subtilis (BS) on plasma biochemical and immune indices and fecal microflora of finishing pigs. A total of 120 Duroc×Landrace×Yorkshire crossbred finishing pigs with an average body weight of (62.90±2.37) kg were randomly divided into 3 groups with 5 replicates per group and 8 pigs per replicate. The control group (group A) was fed a basal diet, and the experimental groups (groups B and C) were fed the basal diets supplemented with 100 and 200 mg/kg BS, receptivity. The preparation period was 7 days and formal experiment period was 63 days. The results showed as follows:1) compared with the control group, the plasma alkaline phosphatase activity of finishing pigs in groups B and C was extremely significantly decreased (P<0.01); the plasma globulin content of finishing pigs in group B was significantly decreased (P<0.05) and the plasma albumin content was significantly increased (P<0.05). 2) The plasma immunoglobulin A content and porcine circovirus antibody level of finishing pigs in group C were extremely significantly higher than those in control group (P<0.01). 3) The addition of Bacillus subtilis in diets had no significant effect on the endotoxin content in plasma and muscle of finishing pigs (P>0.05). 4) Compared with the control group, the relative content of Bifidobacteria in faeces of finishing pigs in groups B and C was significantly increased (P<0.05), the relative content of Bacillus megaterium in faeces of finishing pigs in group B was significantly increased (P<0.05), and the relative content of Clostridium perfringens in faeces of finishing pigs in group C was extremely significantly decreased (P<0.01). It can be seen that adding BS to diet can improve metabolism, enhance immune function and optimize intestinal flora of finishing pigs to a certain extent.
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