Abstract：The aim of this study was to investigate the effects of Bacillus subtilis (BS) on plasma biochemical and immune indices and fecal microflora of finishing pigs. A total of 120 Duroc×Landrace×Yorkshire crossbred finishing pigs with an average body weight of (62.90±2.37) kg were randomly divided into 3 groups with 5 replicates per group and 8 pigs per replicate. The control group (group A) was fed a basal diet, and the experimental groups (groups B and C) were fed the basal diets supplemented with 100 and 200 mg/kg BS, receptivity. The preparation period was 7 days and formal experiment period was 63 days. The results showed as follows:1) compared with the control group, the plasma alkaline phosphatase activity of finishing pigs in groups B and C was extremely significantly decreased (P<0.01); the plasma globulin content of finishing pigs in group B was significantly decreased (P<0.05) and the plasma albumin content was significantly increased (P<0.05). 2) The plasma immunoglobulin A content and porcine circovirus antibody level of finishing pigs in group C were extremely significantly higher than those in control group (P<0.01). 3) The addition of Bacillus subtilis in diets had no significant effect on the endotoxin content in plasma and muscle of finishing pigs (P>0.05). 4) Compared with the control group, the relative content of Bifidobacteria in faeces of finishing pigs in groups B and C was significantly increased (P<0.05), the relative content of Bacillus megaterium in faeces of finishing pigs in group B was significantly increased (P<0.05), and the relative content of Clostridium perfringens in faeces of finishing pigs in group C was extremely significantly decreased (P<0.01). It can be seen that adding BS to diet can improve metabolism, enhance immune function and optimize intestinal flora of finishing pigs to a certain extent.
GIANG H H,VIET T Q,OGLE B,et al.Growth performance,digestibility,gut environment and health status in weaned piglets fed a diet supplemented with potentially probiotic complexes of lactic acid bacteria[J].Livestock Science,2010,129(1/2/3):95-103.
[36]
MANEEWAN C,YAMAUCHI K E,THIRABUNYANON M,et al.Development of Bacillus subtilis MP and effective utilization on productivity and microorganisms in feces of suckling piglets[J].Journal of Applied Research in Veterinary Medicine,2009,9(4):382-387.
[32]
LI Z,WANG W,LV Z,et al.Bacillus subtilis and yeast cell wall improve the intestinal health of broilers challenged by Clostridium perfringens[J].British Poultry Science,2017,58(6):635-643.
[8]
CHEN X L,WANG J K,WU Y M,et al.Effects of chemical treatments of rice straw on rumen fermentation characteristics,fibrolytic enzyme activities and populations of liquid-and solid-associated ruminal microbes in vitro[J].Animal Feed Science and Technology,2008,141(1/2):1-14.
GUO J R,DOND X F,LIU S,et al.Effects of long-term Bacillus subtilis CGMCC 1.921 supplementation on performance,egg quality,and fecal and cecal microbiota of laying hens[J].Poultry Science,2017,96(5):1280-1289.
LIU X,PENG C Y,QU X Y,et al.Effects of Bacillus subtilis C-3102 on production,hatching performance,egg quality,serum antioxidant capacity and immune response of laying breeders[J].Journal of Animal Physiology Animal Nutrition,2019,103(1):182-190.
OH J K,PAJARILLO E A B,CHAE J P,et al.Protective effects of Bacillus subtilis against Salmonella infection in the microbiome of Hy-Line brown layers[J].Asian-Australasian Journal of Animal Sciences,2017,30(9):1332-1339.
LUDIDI S,JONKERS D,ELAMIN E,et al.The intestinal barrier in irritable bowel syndrome:subtype-specific effects of the systemic compartment in an in vitro model[J].PLoS One,2015,10(5):e0123498.
FORTE C,MOSCATI L,ACUTI G,et al.Effects of dietary Lactobacillus acidophilus and Bacillus subtilis on laying performance,egg quality,blood biochemistry and immune response of organic laying hens[J].Journal of Animal Physiology and Animal Nutrition,2016,100(5):977-987.
[12]
LI W F,RAJPUT I R,XU X,et al.Effects of probiotic (Bacillus subtilis) on laying performance,blood biochemical properties and intestinal microflora of Shaoxing duck[J].International Journal of Poultry Science,2011,10(8):583-589.
GUO X H,LI D F,LU W Q,et al.Screening of bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs[J].Antonie van Leeuwenhoek,2006,90(2):139-146.
[39]
LEE S H,INGALE S L,KIM J S,et al.Effects of dietary supplementation with Bacillus subtilis LS 1-2 fermentation biomass on growth performance,nutrient digestibility,cecal microbiota and intestinal morphology of weanling pig[J].Animal Feed Science and Technology,2014,188:102-110.
[13]
MA Q G,GAO X,ZHOU T,et al.Protective effect of Bacillus subtilis ANSB060 on egg quality,biochemical and histopathological changes in layers exposed to aflatoxin B1[J].Poultry Science,2012,91(11):2852-2857.
[18]
SUN P,WANG J Q,ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of preweaning calves[J].Journal of Dairy Science,2010,93(12):5851-5855.
[27]
LI F Z,LI S W,LIU X F,et al.The structure of WbnH in a near active state[J].Protein & Cell,2015,6(8):615-618.
[1]
BARTON M D.Impact of antibiotic use in the swine industry[J].Current Opinion in Microbiology,2014,19:9-15.
[5]
AFRC R F.Probiotics in man and animals[J].Journal of Applied Bacteriology,2010,66(5):365-378.
