Effects of Gossypol Acetate on Rumen Microbial Population and Digestion and Metabolism in Sheep
LI Yuqi1, WANG Caidie1, TUNISA·Maitisaiyidi1, WANG Shichang1, YANG Hongjian2, YANG Kailun1
1. Xinjiang Key Laboratory of Meat & Milk Production Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China;
2. State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100193, China
Abstract：This experiment aimed to explore the effects of gossypol acetate on rumen microbial population, digestion and metabolism in sheep by analyzing microbial population, rumen fluid metabolism indexes, and activity of some hydrolases in rumen after adding gossypol acetate. Eight Kazakh sheep aged about 3-years-old, with permanent rumen fistula, and an average body weight of (49.13±4.70) kg, were randomly divided into two groups (control group and trial group, four animals in each group). The experiment was divided into two phases, with 25 d in each phase. All sheep were fed the same powder concentrate, the feeding account of powder concentrate was 1.50% of body weight per sheep per day, wheat straw and alfalfa were 1.00% of body weight, corn silage was 0.05% of body weight (dry matter basis), and mixed feeding and free drinking water. On the basis of this diet, trial group in period Ⅰ and period Ⅱ added gossypol acetate (600 mg per sheep per day and 1 200 mg per sheep per day, respectively). The results showed that there was no significant difference in the rumen pH after adding gossypol acetate (P>0.05). The rumen ammonia nitrogen concentration in the trial group was significantly higher than that in the control group before feeding gossypol acetate (0 h) (P<0.05). The concentration of volatile fatty acid in the trial group was significantly lower than that in the control group at 6.0, 9.0 and 12.0 h after feeding (P<0.05). During the period Ⅰ, the total population of rumen fluid protozoa in the trial group was extremely significantly lower than that in the control group (P<0.01). The rumen cellulase activity in the trial group was significantly higher than that in the control group at 3.0 h after feeding (P<0.05). The protease activity was extremely significantly lower than that in the control group at 9.0 and 12.0 h after feeding (P<0.01). During the period Ⅱ, the protease activity in the trial group was significantly lower than that in the control group at 6.0 h after feeding (P<0.05), and extremely significantly lower than that in the control group at 12.0 h after feeding (P<0.01). After adding gossypol acetate, the gossypol concentration in rumen was the highest at 1.5 and 3.0 h after feeding. Therefore, the rumen protozoa population of sheep significantly reduces after initial intake of gossypol acetate. Gossypol acetate affects protein digestion and metabolism in rumen by inhibiting the protease activity, and reducing the concentration of valeric acid, isovaleric acid and isobutyric acid in rumen fluid. This effect mainly occurs at 6.0 h after intaking gossypol acetate. Gossypol acetate does not have significant effects on rumen fluid cellulase and amylase activities in sheep as a whole.
李钰琪, 王彩蝶, 屯妮萨·麦提赛伊迪, 王世昌, 杨红建, 杨开伦. 醋酸棉酚对绵羊瘤胃微生物数量及消化代谢的影响[J]. 动物营养学报, 2019, 31(7): 3421-3430.
LI Yuqi, WANG Caidie, TUNISA·Maitisaiyidi, WANG Shichang, YANG Hongjian, YANG Kailun. Effects of Gossypol Acetate on Rumen Microbial Population and Digestion and Metabolism in Sheep. Chinese Journal of Animal Nutrition, 2019, 31(7): 3421-3430.
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