Abstract：This study was conducted to explore the effects of insulin injection on blood glucose concentration and feeding status of different breed chickens. Experiment 1:one hundred and twenty male Hy-line laying hens (abbreviated as laying hens), silky black bone chickens (abbreviated as silky chickens) and Arbor Acres (AA) broiler chickens (abbreviated as broiler chickens) at 21 days of age were randomly divided into 4 groups, respectively, and the 4 groups were insulin fasting group (n=60, of which 12 chickens were used to measure dynamic changes of blood glucose concentration, and rest 48 chickens were slaughtered at 0, 15, 120 and 240 min after insulin injection and 12 chickens per time point, and 10 mL jugular blood were collected to determine serum insulin concentration), phosphate buffered saline (PBS) fasting group (n=12), insulin feeding group (n=24) and PBS feeding group (n=24). All chickens fasted for 16 h before test, injection dose was 5 IU/kg insulin or the same dosage of PBS, chickens in fasting groups were continued to fasted after injection and used to determine dynamic changes of blood glucose concentration, and chickens in feeding groups were feeding immediately after injection, one half were used to measure dynamic changes of blood glucose concentration and another half were used to statistics feeding status. Experiment 2:forty eight laying hens, silky chickens and broiler chickens at 44 days of age were randomly divided into 2 groups, respectively, insulin group (n=24) and PBS group (n=24). All chickens fasted for 16 h before test, injection dose was 2.3 IU/kg insulin or the same dosage of PBS, and feeding after 120 min of injection, one half in each group were used to measure dynamic changes of blood glucose concentration, and another half were used to statistics feeding statues. The results showed as follows:1) insulin injection significantly decreased blood glucose concentration of all breed chickens at 21 days of age in fasting state (P<0.05). Blood glucose concentration of all breed chickens was significantly lower than basic blood glucose concentration after 15 min of injection (P<0.05), and continued to drop until 120 min. Blood glucose concentration of broilers was decreased more sharply compared with laying hens and silky chickens, and recovery of blood glucose concentration was significantly lower than that of laying hens and silky chickens after 120 min (P<0.05). Serum insulin concentration of broilers at different time points was higher than that of laying hens and silky chicken after insulin injection. 2) Compared with basic blood glucose concentration, feeding immediately after insulin injection significantly decreased blood glucose concentration of laying hens at 21 days of age at 60 and 120 min (P<0.05), blood glucose concentration of broilers after insulin injection 30 and 60 min was significantly increased (P<0.05). 3) Feed immediately after insulin injection had no significant effect on cumulative feed intake of all breed chickens at 21 days of age in 240 min (P>0.05), and significantly decreased feed intake of laying hens and silky chickens after insulin injection from 120 to 240 min (P<0.05), significantly increased feed intake of broilers after insulin injection from 30 to 60 min (P<0.05). 4) Feeding after insulin injection 120 min significantly decreased food intake of all breed chickens at 44 days of age (P<0.05). In summary, there are differences in insulin sensitivity and blood glucose concentration response changes among different breed chickens, blood glucose regulation ability of silky chickens and laying hens are better than those of broilers. Insulin injection has significant effects on feed intake of chickens, and there are difference in influence on feed intake.
王焕杰, 杜鹏飞, 张吉昌, 朱瑶, 姬杰菲, 陈文, 黄艳群. 注射胰岛素对不同品种鸡血糖浓度和采食情况的影响[J]. 动物营养学报, 2020, 32(5): 2164-2175.
WANG Huanjie, DU Pengfei, ZHANG Jichang, ZHU Yao, JI Jiefei, CHEN Wen, HUANG Yanqun. Effects of Insulin Injection on Blood Glucose Concentration and Feeding Status of Different Breed Chickens. Chinese Journal of Animal Nutrition, 2020, 32(5): 2164-2175.
VANDENBERG M A,WEBBER M J.Biologically inspired and chemically derived methods for glucose-responsive insulin therapy[J].Advanced Healthcare Materials,2019,8(12):1801466.
[13]
SHIRAISHI J I,YANAGITA K,FUJITA M,et al.Central insulin suppresses feeding behavior via melanocortins in chicks[J].Domest Anim Endocrinol,2008,34(3):223-228.
[15]
LEPKOVSKY S,LEN R,KOIKE T,et al.Effects of protamine zinc insulin on chickens[J].American Journal of Physiology-Legacy Content,1965,208(3):589-592.
[17]
SHANAWANY M M,AL-KHAZRAJI A K,HAMED O,et al.Effect of protamine zinc insulin on feed intake and weight gain in broiler chicks[J].Experimental Physiology,1979,64(4):291-295.
[18]
LIU L,XU S H,WANG X J,et al.Peripheral insulin doesn't alter appetite of broiler chicks[J].Asian-Australasian Journal of Animal Sciences,2016,29(9):1294-1299.
[19]
SUMNERS L H,ZHANG W,ZHAO X,et al.Chickens from lines artificially selected for juvenile low and high body weight differ in glucose homeostasis and pancreas physiology[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2014,172:57-65.
[21]
SCANES C G.Perspectives on the endocrinology of poultry growth and metabolism[J].General and Comparative Endocrinology,2009,163(1/2):24-32.
[23]
RICE B B,ZHANG W,BAI S P,et al.Insulin-induced hypoglycemia associations with gene expressionchanges in liver and hypothalamus of chickens from lines selected for low or high body weight[J].General and Comparative Endocrinology,2014,208:1-4.
[6]
REUSCH J E B,MANSON J A E.Management of Type 2 diabetes in 2017 getting to goal[J].JAMA,2017,317(10):1015-1016.
[12]
HONDA K,KAMISOYAMA H,SANEYASU T,et al.Central administration of insulin suppresses food intake in chicks[J].Neurosci Lett,2007,423(2):153-157.
[14]
SMITH C J V,BRIGHT-TAYLOR B.Does a glucostatic mechanism for food intake control exist in chickens?[J].Poultry Science,1974,53(5):1720-1724.
[16]
PROSZKOWIEC-WEGLARZ M,DUPONT J,RIDEAU N,et al.Insulin immuno-neutralization decreases food intake in chickens without altering hypothalamic transcripts involved in food intake and metabolism[J].Poultry Science,2017,96(12):4409-4418.
[20]
MCMURTRY J P,ROSEBROUGH R W,STEELE N C.A homologous radioimmunoassay for chicken insulin[J].Poultry Science,1983,62(4):697-701.
[22]
谢德彪,陈鹭江.鸡血糖含量变化特点的研究[J].中国畜牧杂志,1996,32(1):26-28.
[24]
SIMON J,GUILLAUMIN S,CHEVALIER B,et al.Plasma glucose-insulin relationship in chicken lines selected for high or low fasting glycaemia[J].British Poultry Science,2000,41(4):424-429.
[30]
徐运杰.神经和激素对家禽采食量的调控[J].江西饲料,2011(4):1-4.
[31]
SHIRAISHI J I,YANAGITA K,FUKUMORI R,et al.Comparisons of insulin related parameters in commercial-type chicks:evidence for insulin resistance in broiler chicks[J].Physiology & Behavior,2011,103(2):233-239.
[32]
YUAN LX,NI Y D,BARTH S,et al.Layer and broiler chicks exhibit similar hypothalamic expression of orexigenic neuropeptides but distinct expression of genes related to energy homeostasis and obesity[J].Brain Research,2009,1273:18-28.
[26]
WILMOT E,IDRIS I.Early onset type 2 diabetes:risk factors,clinical impact and management[J].Therapeutic Advances in Chronic Disease,2014,5(6):234-244.
[34]
ZHANG K Y,XIE N,YE H Q,et al.Function of glucose transporter in the regulation of blood glucose homeostasis[J].Current Biotechnology,2019,9(1):27-33.
BRAUN E J,SWEAZEA K L.Glucose regulation in birds[J].Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2008,151(1):1-9.
[8]
DATAR S P,BHONDE R R.Modeling chick to assess diabetes pathogenesis and treatment[J].The Review of Diabetic Studies,2011,8(2):245-253.
[9]
HENQUIN J C.Regulation of insulin secretion:a matter of phase control and amplitude modulation[J].Diabetologia,2009,52(5):739-751.
[25]
DESPRÉS J P,LEMIEUX I.Abdominal obesity and metabolic syndrome[J].Nature,2006,444(7121):881-887.
[33]
宋庆华.肉鸡低血糖-尖峰死亡综合征的防治[J].养殖与饲料,2017(10):88-90.
[3]
AKIBA Y,CHIDA Y,TAKAHASHI T,et al.Persistent hypoglycemia induced by continuous insulin infusion in broiler chickens[J].British Poultry Science,1999,40(5):701-705.
[5]
YU J C,ZHANG Y Q,BOMBA H,et al.Stimuli-responsive delivery of therapeutics for diabetes treatment[J].Bioengineering & Translational Medicine,2016,1(3):323-337.
[10]
ZHAO N,LIU CC,VAN INGELGOM A J,et al.Apolipoprotein E4 impairs neuronal insulin signaling by trapping insulin receptor in the endosomes[J].Neuron,2017,96(1):115-129.e5.
[29]
WOLFGANG M J,LANE M D.Control of energy homeostasis:role of enzymes and intermediates of fatty acid metabolism in the central nervous system[J].Annual Review of Nutrition,2006,26:23-44.
