Abstract：The aim of this experiment was to investigate the regulating effects of yeast culture (YC) on subacute ruminal acidosis (SARA) fermentation model in vitro in goats. Six different levels of YC[0 (control), 0.5%, 1.0%, 1.5%, 2.0% and 2.5%] were supplemented into substrate with different non-fibrous carbohydrates (NFC)/neutral detergent fiber (NDF) at 1.19 (conventional substrate) and 2.30 (high NFC substrate), respectively. Rumen fermentation parameters were determined, and moreover, the multi-index composite index (MFAEI) was evaluated. The results showed as follows:1) under high NFC substrate condition, the duration of pH lower than 5.8 was longer than 3 h, which indicated that the SARA model was established successfully in vitro. The pH during 9 to 12 h was stabilized with YC supplementation. In addition, the content of lactate at 12 and 24 h significantly decreased (P<0.05), the content of ammoniacal nitrogen (NH3-N) at 9 and 12 h significantly decreased (P<0.05), the content of microbial protein (MCP) at 12 h significantly increased (P<0.05), the percentage of propionic acid at 3, 6, 12 and 24 h significantly increased (P<0.05), and the content of total volatile fatty acids (TVFA) at 24 h significantly increased (P<0.05). 2) Under conventional substrate condition, the pH at 24 h in rumen fermentation fluid was also stabilized with YC supplementation. At 12 h, the content of lactate significantly decreased (P<0.05); at 9 h, the content of NH3-N significantly increased (P<0.05); at 6 and 9 h, the content of MCP significantly increased (P<0.05); during 3 to 24 h, the content of TVFA significantly increased (P<0.05). 3) The MFAET results showed that 1.5% YC could stabilize in vitro rumen fermentation under conventional substance condition; 2.0% YC supplementation could relive SARA under high NFC substance condition. In conclusion, YC supplementation has the best effect under high NFC substance condition, and relive SARA by improving the pH and reducing the content of lactic acid in rumen fermentation fluid, which indicate that YC has the potential to alleviate SARA.
任胜男, 金亚倩, 赵芳芳, 王洪荣. 酵母培养物对山羊亚急性瘤胃酸中毒体外发酵模型的调控作用[J]. 动物营养学报, 2020, 32(5): 2263-2272.
REN Shengnan, JIN Yaqian, ZHAO Fangfang, WANG Hongrong. Regulating Effects of Yeast Culture on Subacute Ruminal Acidosis Fermentation Model in Vitro in Goats. Chinese Journal of Animal Nutrition, 2020, 32(5): 2263-2272.
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