Effects of Diet Composition and Antibiotics on Growth Performance and Intestinal Health of Weaned Piglets
XIA Zou, FENG Jiangxin, JIANG Junjie, CHEN Daiwen, YU Bing, HE Jun, YU Jie, MAO Xiangbing, LUO Yuheng, HUANG Zhiqing, LUO Junqiu, YAN Hui, ZHENG Ping
Key Lab for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China
Abstract：This experiment was conducted to study the effects of diet composition and antibiotics on growth performance and intestinal health in weaned piglets. A total of 72 piglets (Duroc×Landrace×Yorkshire) were selected and assigned to the 4 treatments with 6 replicates per treatment and 3 weaned piglets per replicate. The trial was designed with 2×2 factors, including two diets "simple diets and complex diets" with or without antibiotics (75 mg/kg chlortetracycline and 50 mg/kg kitasamycin). The experiment lasted for 21 days. The results showed as follows:1) compared with simple diets, complex diets significantly decreased the feed/gain (F/G) and diarrhea rate of piglets from days 1 to 7 and 1 to 21(P<0.05), significantly increased the average daily gain (ADG) of piglets from days 1 to 7 (P<0.05), significantly increased the ratio of villus height to crypt depth in ileum and the number of goblet cells in jejunum (P<0.05), upregulated the relative expression of occludin (OCLN) mRNA in jejunum (P<0.05), significantly increased the contents of interleukin-10 (IL-10) (P<0.05) and transforming growth factor-β1 (TGF-β1) in jejunum, and significantly decreased the contents of interleukin-1β (IL-1β) in ileum (P<0.05). 2) Compared with no antibiotic diet, antibiotic diet significantly increased the ADG of piglets from days 1 to 7 and days 1 to 21 (P<0.05), the average daily feed intake (ADFI) from days 1 to 21 and final body weight on day 22 (P<0.05), significantly decreased the F/G and the diarrhea rate of piglets from days 1 to 7 and days 1 to 21 (P<0.05), significantly increased the ratio of villus height to crypt depth in ileum (P<0.05), significantly increased the number of goblet cells in jejunum and ileum (P<0.05), and significantly decreased the relative expressions of TLR2 mRNA in jejunum and ileum (P<0.05). In conclusion, complex diets can increase the growth performance of weaned piglets, decrease the diarrhea rate, and enhance the intestinal immune barrier function; while antibiotics can inhibit diarrhea, but has an adverse effect on intestinal barrier function of weaned piglets.
KATS L J,TOKACH M D,GOODBAND R D,et al.Influence of weaning weight and growth during the first week postweaning on subsequent pig performance[J].Kansas Agricultural Experiment Station Research Reports,1992(10),doi:10.4148/2378-5977.6731.
[9]
FUNDERBURKE D W,SEERLEY R W.The effects of postweaning stressors on pig weight change,blood,liver and digestive tract characteristics[J].Journal of Animal Science,1990,68(1):155-162.
[11]
PIERCE J L,CROMWELL G L,LINDEMANN M D,et al.Effect of spray-dried animal plasma and immunoglobulins on performance of early weaned pigs[J].Journal of Animal Science,2005,83(12):2876-2885.
[19]
CORL B A,HARRELL R J,MOON H K,et al.Effect of animal plasma proteins on intestinal damage and recovery of neonatal pigs infected with rotavirus[J].The Journal of Nutritional Biochemistry,2007,18(12):778-784.
[2]
ZHANG Y,ZHENG P,YU B,et al.Dietary spray-dried chicken plasma improves intestinal barrier function and modulates immune status in weaning piglets[J].Journal of Animal Science,2016,94(1):173-184.
[7]
WOLTER B F,ELLIS M.The effects of weaning weight and rate of growth immediately after weaning on subsequent pig growth performance and carcass characteristics[J].Canadian Journal of Animal Science,2001,81(3):363-369.
[12]
NARANJO V D,BIDNER T D,SOUTHERN L L.Comparison of dried whey permeate and a carbohydrate product in diets for nursery pigs[J].Journal of Animal Science,2010,88(5):1868-1879.
STRARKE I C,PIEPER R,NEUMANN K,et al.The impact of high dietary zinc oxide on the development of the intestinal microbiota in weaned piglets[J].FEMS Microbiology Ecology,2014,87(2):416-427.
[29]
JAYALAKSHMI K,PARAMASIVAM M,SASIKALA M,et al.Review on antibiotic residues in animal products and its impact on environments and human health[J].Journal of Entomology and Zoology Studies,2017,5(3):1446-1451.
[31]
PUHL N J,UWIERA R R E,YANKE L J,et al.Antibiotics conspicuously affect community profiles and richness,but not the density of bacterial cells associated with mucosa in the large and small intestines of mice[J].Anaerobe,2012,18(1):67-75.
[34]
NAQI S A,SAHIN N,WAGNER G,et al.Adverse effects of antibiotics on the development of gut-associated lymphoid tissues and the serum immunoglobulins in chickens[J].American Journal of Veterinary Research,1984,45(7):1425-1429.
[17]
GAO Y Y,JIANG Z Y,LIN Y C,et al.Effects of spray-dried animal plasma on serous and intestinal redox status and cytokines of neonatal piglets[J].Journal of Animal Science,2011,89(1):150-157.
WANG H F,WANG J L,WANG C,et al.Effect of bamboo vinegar as an antibiotic alternative on growth performance and fecal bacterial communities of weaned piglets[J].Livestock Science,2012,144(1/2):173-180.
HILL D A,HOFFMANN C,ABT M C,et al.Metagenomic analyses reveal antibiotic-induced temporal and spatial changes in intestinal microbiota with associated alterations in immune cell homeostasis[J].Mucosal Immunology,2010,3(2):148-158.
