Effects of Compound Plant Extracts on Rumen Fermentation of Holstein Cows in Vitro
GUO Chenyang1, ZHANG Tenglong1, XU Tengteng1, AO Changjin1, WANG Lifang2
1. College of Animal Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China;
2. Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Autonomous Region, Hohhot 010031, China
Abstract：This experiment was conducted to study the effects of dietary compound plant extracts on rumen fermentation of Holstein cows in vitro. The rumen fluid was collected from 4 healthy Holstein cows with the same lactation period and similar body condition as the rumen fluid donor. The compound plant extracts were the alcohol extracts for dandelion, Forsythia suspense, honeysuckle and motherwort, which prepared according to the weight ratio of 1:1:1:1. Weighted 2.0 g air-dried total mixed ration (TMR) in artificial rumen culture bottle, then supplemented with 0 (control group), 0.03%, 0.30% and 3.00% compound plant extracts (dry matter basis), and the pH and concentrations of microbial protein (MCP), ammoniacal nitrogen (NH3-N), volatile fatty acid (VFA) and dry matter degradation rate were measured at 2, 4, 6, 8, 12 and 24 h, respectively, and the multiple-factors associative effects index (MFAEI) was used for evaluation. The results showed as follows:1) there was no significant difference in pH among all groups at each time point (P>0.05). At 8 h, the NH3-N concentration of 0.03% and 3.00% dosage groups was significantly higher than that of other two groups (P<0.05). At 2 h, the MCP concentration of 0.03% dosage group was significantly lower than that of 0.30% and 3.00% dosage groups (P<0.05); at 12 h, the MCP concentration of 0.30% and 3.00% dosage groups was significantly lower than that of the control group (P<0.05). 2) At 8 h, the total volatile fatty acid (TVFA) concentration of 0.03%, 0.30% and 3.00% dosage groups was significantly higher than that of the control group (P<0.05), and the propionic acid concentration of 0.03%, 0.30% and 3.00% dosage groups was significantly higher than that of the control group (P<0.05). 3) At 4 h, the dry matter degradation rate of 0.03% and 0.30% dosage groups was significantly higher than that of the control group (P<0.05). 4) At 6 h, the gas production of 3.00% dosage group was significantly higher than that of 0.03% and 0.30% dosage groups (P<0.05); at 8 h, the gas production of 0.30% and 3.00% dosage groups was significantly higher than that of control group and 0.03% dosage group (P<0.05); at 12 and 24 h, the gas production of 0.03%, 0.30% and 3.00% dosage groups was significantly higher than that of the control group (P<0.05). 5) The order of MFAEI from high to low was 0.30% dosage group>3.00% dosage group>0.03% dosage group. Therefore, under in vitro culture condition, the addition of compound plant extracts of dandelion, Forsythia suspense, motherwort and honeysuckle can effectively regulate the rumen fermentation of Holstein cows, and the optimum additive amount is 0.30%.
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