Abstract:In this experiment, different source essential oils were added to broiler diets to study their effects on growth performance, immune function and intestinal development. Single factor test design was adopted in this test, a total of 504 one-day-old broilers (mixed male and female) with similar body weight were randomly divided into 6 groups (7 replicates in each group and 12 replicates in each group):control group (CK group) was fed a basic diet; antibiotic group (AG group) was added with antibiotics (Clomithromycin) at a dosage of 5 mg/kg; plant essential oil groups[EO1 group (the effective component was thymol, and effective content was 1.7%), EO2 group (the effective component were carvol and cinnamaldehyde, and effective content were 5% and 3%) and EO3 group (the effective component were cinnamaldehyde and thymol, and effective content was 10%), EO4 group (the effective component were thymol and carvol, and effective content was 25%)] were added with four plant essential oils at dosages of 150, 100, 100 and 200 mg/kg. The test lasted for 42 days. The results showed as follows:1) compared with the CK group, the average daily weight gain and body weight in AG group, EO3 group and EO4 group increased significantly (P<0.05). There was no significant difference in spleen index, bursa of Fabricius index and thymus index (P>0.05). 2) Compared with the CK group, the content of immunoglobulin G in serum in AG group increased significantly (P<0.05), and serum immunoglobulin M content in AG group, EO3 group and EO4 group also increased significantly (P<0.05). 3) Compared with the CK group, the crypt depth of duodenum in AG group decreased significantly (P<0.05), and the villus height of ileum significantly elevated (P<0.05); compared with the CK group, jejunal villus height in EO3 group increased significantly (P<0.05); crypt depth in AG group, EO1 group, EO2 group and EO4 group decreased significantly (P<0.05); the ratio of jejunal villus height to crypt depth in AG group was significantly higher than that in CK group (P<0.05). In conclusion, it is found that plant essential oil can promote the growth performance and immune function of broilers. Among them, EO3 group has the best effect, which is close to the effect of antibiotic feeding, indicating that it has a potential as a substitute of antibiotics.
周洪彬, 魏景坤, 刘洋, 王月, 徐良梅. 植物精油对肉仔鸡生长性能、免疫功能及肠道发育的影响[J]. 动物营养学报, 2020, 32(8): 3887-3895.
ZHONG Hongbin, WEI Jingkun, LIU Yang, WANG Yue, XU Liangmei. Effects of Plant Essential Oil on Growth Performance, Immune Function and Intestinal Development of Broilers. Chinese Journal of Animal Nutrition, 2020, 32(8): 3887-3895.
LIVEIRA G L,CARDOSO S K,JÚNIOR C R O l,et al.Chemical study and larvicidal activity against Aedes aegypti of essential oil of Piper aduncum L.(Piperaceae)[J].Anais da Academia Brasileira de Ciências,2013,85(4):1227-1234.
[7]
BENBELAÏD F,KHADIR A,BENDAHOU M,et al.Composition and antimicrobial activity of Cistus munbyi essential oil:an endemic plant from Algeria[J].Journal of Forestry Research,2017,28(6):1129-1134.
[12]
WALTER B M,BILKEI G.Immunostimulatory effect of dietary oregano etheric oils on lymphocytes from growth-retarded,low-weight growing-finishing pigs and productivity[J].Tijdschrift Voor Diergeneeskunde,2004,129(6):178-181.
[13]
ZOU Y,XIANG Q H,WANG J,et al.Oregano essential oil improves intestinal morphology and expression of tight junction proteins associated with modulation of selected intestinal bacteria and immune status in a pig model[J].BioMed Research International,2016,2016:5436738.
[15]
LI S Y,RU Y J,LIU M,et al.The effect of essential oils on performance,immunity and gut microbial population in weaner pigs[J].Livestock Science,2012,145(1/2/3):119-123.
[17]
WEBER G M,MICHALCZUK M,HUYGHEBAERT G,et al.Effects of a blend of essential oil compounds and benzoic acid on performance of broiler chickens as revealed by a meta-analysis of 4 growth trials in various locations[J].Poultry Science,2012,91(11):2820-2828.
[20]
JANG I S,KO Y H,KANG S Y,et al.Effect of a Commercial essential oil on growth performance,digestive enzyme activity and intestinal microflora population in broiler chickens[J].Animal Feed Science and Technology,2007,134(3/4):304-315.
[22]
HASSAN H M A,MOHAMED M A,YOUSSEF A W,et al.Effect of using organic acids to substitute antibiotic growth promoters on performance and intestinal microflora of broilers[J].Asian-Australasian Journal of Animal Sciences,2010,23(10):1348-1353.
MEMARIANI Z,SHARIFZADEH M,BOZORGI M,et al.Protective effect of essential oil of pistacia atlantica desf.On peptic ulcer:role of α-pinene[J].Journal of Traditional Chinese Medicine,2017,37(1):57-63.
[11]
MOHITI-ASLI M,GHANAATPARAST-RASHTI M.Comparison of the effect of two phytogenic compounds on growth performance and immune response of broilers[J].Journal of Applied Animal Research,2017,45(1):603-608.
[21]
JAMROZ D,WERTELECKI T,HOUSZKA M,et al.Influence of diet type on the inclusion of plant origin active substances on morphological and histochemical characteristics of the stomach and jejunum walls in chicken[J].Journal of Animal Physiology and Animal Nutrition,2006,90(5/6):255-268.
[27]
GIANNENAS I,TRIANTAFILLOU E,STAVRAKAKIS S,et al.Assessment of dietary supplementation with carvacrol or thymol containing feed additives on performance,intestinal microbiota and antioxidant status of rainbow trout (Oncorhynchus mykiss)[J].Aquaculture,2012,350-353:26-32.
[30]
FRANCIOSINI M P,CASAGRANDE-PROIETTI P,FORTE C,et al.Effects of oregano (Origanum vulgare L.) and rosemary (Rosmarinus Officinalis L.) aqueous extracts on broiler performance,immune function and intestinal microbial population[J].Journal of Applied Animal Research,2016,44(1):474-479.
[31]
CASTILLO M,MARTÍN-ORÚE S M,ROCA M,et al.The response of gastrointestinal microbiota to avilamycin,butyrate,and plant extracts in early-weaned pigs[J].Journal of Animal Science,2006,84(10):2725-2734.
[33]
ZHANG K Y,YAN F,KEEN C A,et al.Evaluation of microencapsulated essential oils and organic acids in diets for broiler chickens[J].International Journal of Poultry Science,2005,4(9):612-619.
[35]
PEI R S,ZHOU F,JI B P,et al.Evaluation of combined antibacterial effects of eugenol,cinnamaldehyde,thymol,and carvacrol against E. coli with an Improved Method[J].Journal of Food Science,2009,74(7):M379-M383.
[8]
MONZOTE L,SCULL R,COS P,et al.Essential oil from Piper aduncum:chemical analysis,antimicrobial assessment,and literature review[J].Medicines,2017,4(3):49.
[18]
BOTSOGLOU N A,FLOROU-PANERI P,CHRISTAKI E,et al.Effect of dietary oregano essential oil on performance of chickens and on iron-induced lipid oxidation of breast,thigh and abdominal fat tissues[J].British Poultry Science,2002,43(2):223-230.
[28]
MAGNADÓTTIR B.Innate immunity of fish (overview)[J].Fish & Shellfish Immunology,2006,20(2):137-151.
[6]
DI VAIO C,GRAZIANI G,GASPARI A,et al.Essential oils content and antioxidant properties of peel ethanol extract in 18 lemon cultivars[J].Scientia Horticulturae,2010,126(1):50-55.
[9]
MARCIN?ÁK S,CABADAJ R,POPELKA P,et al.Antioxidative effect of oregano supplemented to broilers on oxidative stability of poultry meat[J].Slovenian Veterinary Research,2008,45(2):61-66.
[16]
ZHENG Z L,TAN J Y W,LIU H Y,et al.Evaluation of oregano essential oil (Origanum heracleoticum L.) on growth,antioxidant effect and resistance against aeromonas hydrophila in channel catfish (Ictalurus punctatus)[J].Aquaculture,2009,292(3/4):214-218.
CHAMORRO S,ROMERO C,BRENES A,et al.Impact of a sustained consumption of grape extract on digestion,gut microbial metabolism and intestinal barrier in broiler chickens[J].Food & Function,2019,10(3):1444-1454.
BOUCHEKRIT M,LAOUER H,HAJJI M,et al.Essential oils from Elaeoselinum asclepium:chemical composition,antimicrobial and antioxidant properties[J].Asian Pacific Journal of Tropical Biomedicine,2016,6(10):851-857.
[14]
MUELLER K,BLUM N M,KLUGE H,et al.Influence of broccoli extract and various essential oils on performance and expression of xenobiotic-and antioxidant enzymes in broiler chickens[J].British Journal of Nutrition,2012,108(4):588-602.
[24]
DAFWANG I I,COOK M E,SUNDE M L,et al.Bursal,intestinal,and spleen weights and antibody response of chicks fed subtherapeutic levels of dietary antibiotics[J].Poultry Science,1985,64(4):634-639.
[34]
TIIHONEN K,KETTUNEN H,BENTO M H L,et al.The effect of feeding essential oils on broiler performance and gut microbiota[J].British Poultry Science,2010,51(3):381-392.