本试验旨在研究饲粮蛋白质水平对冬毛期水貂血清含氮物质、氮代谢和抗氧化能力的影响。选择90日龄健康雄性水貂60只，随机分成6组（每组10个重复，每个重复1只水貂），分别饲喂蛋白质水平为28%(Ⅰ组)、30%(Ⅱ组)、32%(Ⅲ组)、34%(Ⅳ组)、36%(Ⅴ组)、38%(Ⅵ组)的试验饲粮。预试期7 d，正式期90 d，饲养试验结束后进行屠宰，制备血清并测定相关生化指标。结果表明，Ⅲ组水貂的血清总蛋白、白蛋白、球蛋白含量显著或极显著高于其他各试验组(P<0.05或P<0.01)，其他各试验组间差异均不显著(P>0.05)；Ⅳ组水貂血清总氨基酸含量极显著高于Ⅰ、Ⅱ和Ⅲ组(P<0.01)；Ⅲ组水貂的血清尿素氮含量显著高于Ⅱ、Ⅳ和Ⅴ组(P<0.05)；Ⅴ组水貂的血清谷草转氨酶活性显著高于Ⅱ和Ⅵ组(P<0.05)，与其他试验组差异均不显著(P>0.05)；Ⅳ组水貂的血清谷丙转氨酶活性显著高于Ⅲ组(P<0.05)；各试验组水貂的血清丙二醛含量差异不显著(P>0.05)；Ⅳ、Ⅴ和Ⅵ组水貂的血清超氧化物歧化酶活性显著或极显著高于Ⅱ和Ⅲ组(P<0.05或P<0.01)；血清谷胱甘肽过氧化物酶活性以Ⅳ组最高，显著高于Ⅵ组(P<0.05)，但与其他试验组差异均不显著(P>0.05)。由此得出，冬毛期水貂饲粮蛋白质水平达到34%时，机体的蛋白质合成效率相对较高，抗氧化能力相对较强。
The experiment was conducted to study the effects of dietary protein levels on serum nitrogen heterocyclic compounds, nitrogen metabolism and antioxidant capacity of minks during the winter hair period. Sixty healthy 90-day-old male minks were randomly divided into 6 groups with 10 replicates per group and 1 mink per replicate. The minks were fed the experimental diets with 28% (group Ⅰ), 30% (group Ⅱ), 32% (group Ⅲ), 34% (group Ⅳ), 36% (group Ⅴ) and 38% (group Ⅵ) protein levels, respectively. The trial lasted for 97 d with 7 d of pretrial, and then the minks were slaughtered. The serum was centrifuged and the relative biochemical indices were determined. The results showed as follows: the contents of serum total protein, albumin and globulin in group Ⅲ were significantly higher than those in other groups (P<0.05 or P<0.01), and no significant difference was found among other groups (P>0.05); serum total amino acid content in group Ⅳ was significantly higher than that in groups Ⅰ, Ⅱ and Ⅲ (P<0.01); serum urea nitrogen content in group Ⅲ was significantly higher than that in groups Ⅱ, Ⅳ and Ⅴ (P<0.05); serum glutamic oxaloacetic transaminase activity in group Ⅴ was significantly higher than that in groups Ⅱ and Ⅵ (P<0.05), but no significant difference was found between group Ⅴ and other groups (P>0.05); serum glutamic pyruvic transaminase activity in group Ⅳ was significantly higher than that in group Ⅲ (P<0.05); serum malondialdehyde content had no significant difference among all groups (P>0.05); serum superoxide dismutase activity in groups Ⅳ, Ⅴ, and Ⅵ was significantly higher than that in groups Ⅱ and Ⅲ (P<0.05 or P<0.01); the highest serum glutathione peroxidase activity was found in group Ⅳ which was significantly higher than that in group Ⅵ (P<0.05), but no significant difference was found between group Ⅳ and other groups (P>0.05). In conclusion, when the dietary protein level come up to 34%, the minks in winter hair period have the relatively higher protein synthesis rate and antioxidant capacity.［Chinese Journal of Animal Nutrition, 2011, 23（6）：1052 -1057］
［1］SORENSEN P G, PETERSEN I M, SAND O. Activities of carbohydrate and amino acid metabolizing enzymes from liver of mink (Mustela vison) and preliminary observations on steady state kinetics of the enzymes[J]. Comparative Biochemistry and Physiology, 1995, l12:59-64.
[2]MACDONALD M L, ROGERS Q R, MORRIS J G. Nutrition of the domestic cat, a mammalian carnivore[J]. Annual Review of Nutrition, 1984, 4:521-562.
[3]BRSTING C F. Influence of nutrition on fur quality[J]. Scientifur, 1999, 23(2):106.
[4]DAMGAARD B M, BRSTING C F, FINK R. Effect of dietary protein and carbohydrate supply on feed consumption, growth performance and blood parameters in mink dams during the nursing period[J]. Scientifur, 2004(4)：17-21.
[5]NRC. Nutrient requirements of mink and foxes[S]. Washington, D. C.: National Academy Press, 1982.
[6]李辉,刁其玉,张乃锋,等.同蛋白质来源对早期断奶犊牛消化及血清生化指标的影响(一)[J].动物营养学报,2009,21(1):47-52.
[7]余红心,贾俊静,李琦华,等.不同蛋白质水平日粮对云南武定鸡生长性能及血液生化指标的影响[J].中国饲料,2009,5:24-26.
[8]MUSTONEN A M, PYYKONEN T, PAAKKONEN T, et al. Adaptations to fasting in the American mink (Mustela vison): carbohydrate and lipid metabolism[J]. Comparative Biochemistry and Physiology: Part A, 2005, 140:195-202.
[9]TUITOEK K, YOUNG L G, DELANGE C F M, et al. The effect of reducing excess dietary amino acids on growing- finishing pig performance: an evaluation of the ideal protein concept[J]. Journal of Animal Science, 1997, 75:1575-1583.
[10]何欣,马秋刚,梁福广,等.氨基酸平衡日粮中不同蛋白质水平对生长猪生长性能及血清生化指标的影响[J].中国畜牧杂志,2010,46(21):65-68.
[11]COMA J, CARION D, ZIMMERMAN D R. Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirements of pigs[J]. Journal of Animal Science, 1996, 73:472-481.
[12]CHIKHOU F H，MOLONEY A P, ALLEN P, et al. Long-term effects of cimaterol in friesian steers: Ⅰ. Growth, feed efficiency, and selected traits[J]. Journal of Animal Science, 1993, 71:906-913.
[13]WANG X, QIAO S Y, LIU M. Effects of graded levels of true ileal digestible threonine on performance, serum parameters and immune function of 10 to 25 kg pigs[J]. Animal Feed Science and Technology, 2006, 129:264-278.
[14]SONG K, SHAN A S, LI J P. Effect of different combinations of enzyme preparation supplemented to wheat based diets on growth and serum biochemical values of broiler chickens[J]. ACTA Zoonutrimenta, 2004, 4:25-29.
[15]JUOKSLATHI T, LINDBERG P, TYOPPONEN O. Organ distribution of some clinically important enzymes in mink[J]. Acta Veterinaria Scandinavica, 1980, 21:347-353.
[16]岳双明,周安国,王之盛,等.日粮锌与蛋白质互作对断奶仔猪生产性能以及部分血液生化指标的影响[J].动物营养学报,2009,21(3):279-287.
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