本试验旨在研究饲料中添加不同水平的维生素K3对松浦镜鲤体成分、肉质和抗氧化能力的影响。在以豆粕和鱼粉为蛋白质源的基础饲料（维生素K1含量为0.54 mg/kg）中分别添加0、5、10、20、40、80和2 000 mg/kg的维生素K3（甲萘醌烟酰胺亚硫酸盐），配制成7种试验饲料。选取初始体重为（2.58±0.16） g的松浦镜鲤幼鱼525尾，随机分为7组，每组设3个重复，每重复放养25尾，在水温24 ℃、溶氧7.46~8.61 mg/L条件下饲喂60 d。结果表明，添加不同水平的维生素K3对松浦镜鲤的体成分(水分、粗蛋白质、粗脂肪、灰分含量)以及肝胰脏中丙二醛（MDA）含量均无显著影响（P>0.05）；0 mg/kg组的剪切力显著小于其他各组（P<0.05）；10 mg/kg组的pH24 h显著小于其他各组（P<0.05），滴水损失显著大于0和5 mg/kg组（P<0.05），肝胰脏超氧化物歧化酶(SOD)活性显著大于0和5 mg/kg组（P<0.05），肝胰脏还原型谷胱甘肽（GSH）含量显著大于2 000 mg/kg组（P<0.05）；10和20 mg/kg组的肝胰脏总抗氧化能力（T-AOC）显著大于0、80和2 000 mg/kg组（P<0.05），但二者差异不显著（P>0.05）。通过折线模型对饲料中维生素K3添加水平和肝胰脏中T-AOC进行一元回归分析，得出T-AOC最高时的维生素K3的添加量为16.89 mg/kg。由此得出，饲料中添加维生素K3可提高松浦镜鲤肌肉的剪切力，降低肌肉的系水力；不添加或过量添加维生素K3均会降低松浦镜鲤的抗氧化能力，其适宜添加量为16.89 mg/kg。
This experiment was conducted to study the effects of different dietary vitamin K3 levels on body composition, meat quality and antioxidant capacity of Songpu mirror carp. A total of 525 juvenile Songpu mirror carp with average body weight of (2.58±0.16) g were randomly allocated into 7 groups with 3 replicates per group and 25 fish per replicate. The fish in 7 groups were fed the basal diet (vitamin K1 content was 0.54 mg/kg) supplemented with 0, 5, 10, 20, 40, 80 and 2 000 mg/kg vitamin K3 (menadione nicotinamide bisulfite, MNB), respectively. Under the conditions of water temperature 24 ℃ and dissolved oxygen 7.46 to 8.61 mg/L, the experiment lasted for 60 days. The results showed as follows: different levels of vitamin K3 supplementation could not affect the body composition (moisture, crude protein, ether extract and ash contents) and the hepatopancreas malonyldiadehyde (MDA) content of Songpu mirror carp (P>0.05). The shearing force of Songpu mirror carp in 0 mg/kg group was significantly lower than that in other groups (P<0.05). In 10 mg/kg group, the pH24 h was significantly lower than that in other groups (P<0.05), but the drip loss and superoxide dismutase (SOD) activity in hepatopancreas were significantly higher than those in 0 and 5 mg/kg groups (P<0.05), and the glutathione (GSH) content in hepatopancreas was significantly higher than that in 2 000 mg/kg group (P<0.05); the hepatopancreas total antioxidant capacity (T-AOC) in 10 and 20 mg/kg groups were significantly higher than those in 0, 80 and 2000 mg/kg groups (P<0.05), but no significant difference was found between 10 and 20 mg/kg groups. The linear regression result showed that the requirement for vitamin K3 of Songpu mirror carp which had the highest T-AOC was 16.89 mg/kg diet. In conclusion, adding vitamin K3 in diet can improve the muscle shearing force and reduce the muscle capacity of holding water of Songpu mirror carp. Whether no or excessively adding vitamin K3 can reduce the antioxidant capacity of Songpu mirror carp, and its optimum addition level is 16.89 mg/kg diet.［Chinese Journal of Animal Nutrition, 2011, 23（6）：1058 -1064］
［1］徐积恩.维生素K的研究和应用进展[J].中国医药情报，1995，19(5)：291.
[2]钱云霞.维生素K对鱼类的营养作用[J].水利渔业，2003，23(6)：58.
[3]李元生.维生素K的生物化学功能及饲喂要点[J].山东畜牧兽医，2009，30(1)：20-21.
[4]章正琰.维生素K3对荷瘤小鼠肿瘤及肝脏谷胱甘肽含量的影响[J].癌症，1995，14 (6)：429-431.
[5 ]章正琰，陈本懋，油书桓.维生素K3的抗肿瘤作用及其部分机制的研究[J].癌症，1992，11(6)：483-484.
[6]GRAHL-MADSEN E L. Effects of different levels of vitamin K in diets for cod (Gadus morhua)[J]. Aquaculture, 1997, 151:269-274.
[7]TAVEEKIJAKARN P, MIYAZAKI T, MATSUMOTO M. Studies on vitamin K deficiency in amago salmon, Oncorhynchus rhodurus (Jordan & Mcgregor)[J]. Fish Diseases, 1996, 19(3):209-214.
[8]GRAFT I E, WAAGB R, FIVELSTAD S. A multite study on the effects of dietary vitamin K, vitamin D3 and calcium, and dissolved carbon dioxide on growth, bone minerals, vitamin status and health performance in smolting Atlantic salmon Salmo salar L[J]. Fish Diseases, 2002, 25(10):599-614.
[9]李池陶，张玉勇，贾智英，等.松浦镜鲤与德国镜鲤选育系(F4)的可量性状、鳞片数比较.水产学杂志，2009，22(2)：53-55.
[10]程汉良，吴立新，王亮.鲤鱼的精准营养与健康养殖[J].饲料工业，2002，23(10)：40-43.
[11]赖铭勇.鲤鱼的营养需要与饲料[J].内陆水产，2004，7：13-14.
[12]GOUILLOU-COUSTANS M F, BERGOT P, KAUSHIK S J. Dietary ascorbic acid needs of common carp (Cyprinus carpio) larvae[J]. Aquaculture, 1998, 161:453-461.
[13]陈润生，马小愚，雷得天.肌肉嫩度计的鉴定和应用研究[J].东北农学院学报,1988(3):227-284.
[14]ALLEN C D, FLETCHER D L, NORTHEUTT J K, et al. The relationship of broiler breast color to meat quality and shelf-life[J]. Poultry Science, 1998, 77:361-366.
[15]蒋明，王卫民，文华,等.维生素K3对草鱼生长、体成分和凝血时间的影响[J].淡水渔业，2007，37(2)：61-64.
[16]金光明，译.幼斑节对虾维生素K需要量[J].饲料外畜牧学，1995(5)：16-19.
[17]FAUCONNEAU B, CHMAITILLY J, ANDRE S, et al. Characteristics of rainbow trout flesh:1. Chemical composition and cellularity of muscle and adipose tissues[J]. Sciences des Aliments, 1993, 13 (2):173-187.
[18]王桂瑛，廖国周，文际坤.微营养素调控肉品质的研究进展[J].饲料工业，2003，24(8)：39-42.
[19]MORRISON H, DI MONTE D, NORDENSKJLD M, et al. Induction of cell damage by menadione and benzo(a)pyrene-3,6-quinone in cultures of adult rat hepatocytes and human fibroblasts[J]. Toxicology Letters, 1985, 28(1):37-47.
[20]JOE A, PICHARDO S, PRIETO A I. Toxic cyanobacterial cells containing mieroeystins induce oxidative stress in exposed tilapia fish (Oreochromis sp.) under laboratory conditions[J]. Aquatic Toxicology, 2005, 72:261-271.
[21]李安娜，张迎梅.十二烷基硫酸钠对斑马鱼抗氧化能力的影响[J].四川动物，2008，27(6)：993-995.
[22]MARCHETTI M. Tolerance of high dietary levels of menadione bisulfite-nicotinamide by rainbow trout, Oncorhynchus mykiss[J]. Aquaculture, 1995, 134:137-142.
[23]范红，王雷.还原型谷胱甘肽的临床应用[J].基层医学论坛，2010，14(9)：841-842.
[24]MUKAI K, MORINCOTO H, OKAUCHI Y K. Study of reactions between tocopheroxyl radicals and fatty acids[J]. Lipid, 1993, 28:753-756.
[25]BURTON G W, TRABER M G. Vitamin E: antioxidant activity biokinetics and bioavailability[J]. Annual Review of Nutrition, 1990, 120:357-382.
[26]UDAGAWA M. The effect of dietary vitamin K (phylloquinone and menadione) levels on the vertebral formation in mummichog Fudulus heteroclitus[J]. Fisheries Science, 2001, 67:104-113.
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