本试验旨在探讨饲粮中添加壳聚糖对早期断奶仔猪生长、腹泻和免疫力的影响。试验选择50头21日龄、平均体重（6.62±0.59） kg的断奶仔猪，随机分为5组，每组10个重复，每个重复1头猪。预试期采用大肠杆菌攻毒(1×109个细胞/头)，对照组饲喂基础饲粮，试验组分别在基础饲粮中添加50 mg/kg金霉素和200、300、400 mg/kg壳聚糖。试验期21 d。结果表明：1）与对照组相比，各试验组平均日采食量提高（P>0.05），料重比和腹泻率降低，平均日增重分别提高了15.8%、9.2%、16.8%和21.1%（P>0.05）。2）与对照组相比，各壳聚糖组血清免疫球蛋白G（IgG）和免疫球蛋白M（IgM）水平以及回肠黏膜白介素1β（IL-1β）、白介素6（IL-6）和肿瘤坏死因子α（TNF-α）mRNA相对表达量均升高，金霉素组均下降，其中300和400 mg/kg壳聚糖组的血清IgG和IgM水平与金霉素组差异显著（P<0.05），400 mg/kg壳聚糖组血清IgG水平还与对照组差异显著（P<0.05），壳聚糖组IL-1β和TNF-α mRNA相对表达量与金霉素组差异显著（P<0.05），300和400 mg/kg壳聚糖组IL-6 mRNA表达量与金霉素组差异显著（P<0.05）。结果提示，饲粮中添加壳聚糖能促进仔猪生长，降低腹泻率，提高非特异性免疫力，且作用效果随饲粮中壳聚糖浓度的增加有增强的趋势。
The experiment was conducted to study effects of chitosan on growth performance and immunity in weanling piglets. Fifty piglets with an average body weight of (6.62±0.59) kg were challenged with enterotoxigenic Escherichia coli (1×109 cell per head) during adaptation period and randomly assigned to 5 groups with 10 replicates per group and 1 piglet in each replicate. The piglets in control group were fed basal diet, and the others in experimental groups were fed the basal diet supplemented with 50 mg/kg chlortetracycline, 200, 300 or 400 mg/kg chitosan, respectively. The experiment lasted for 21 days. The results showed as follows: 1) compared with the control group, in every experimental group, average daily feed intake was improved (P>0.05), the ratio of feed to gain and diarrhea ratio were decreased, and the average daily gain was increased by 15.8%, 9.2%, 16.8% and 21.1% (P>0.05), respectively. 2) Compared with the control group, the serum immunoglobulin G （IgG） and immunoglobulin M （IgM） levels, relative expression levels of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) mRNA in ileum mucosa were improved in the chitosan groups, but decreased in the chlortetracycline group. The IgG and IgM levels in the 300 and 400 mg/kg chitosan groups were significant higher than those in the chlortetracycline group (P<0.05), and the IgG level in the 400 mg/kg chitosan group was significant higher than that in the control group (P<0.05). The relative expression level of IL-1β and TNF-α mRNA in the three chitosan groups was significant higher than those in the chlortetracycline group (P<0.05), and the relative expression levels of IL-6 mRNA in the 300 and 400 mg/kg chitosan groups were significant higher than that in the chlortetracycline group (P<0.05). These results indicate that supplemented with 200, 300 and 400 mg/kg chitosan in diets can improve growth performance, decrease diarrhea ratio and enhance non-specific immunity of weanling piglets, furthermore, those effects have enhancing trend with increasing the concentration of chitosan in diets.［Chinese Journal of Animal Nutrition, 2011, 23（10）：1783 -1789］
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