饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响

李美君, 方成堃, 张凯, 李运虎, 方热军

doi:10.3969/j.issn.1006-267x.2012.01.016

动物营养学报 >

2012 , Vol. 24 >Issue 1: 111 - 116

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.01.016

饲料营养

饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响

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1. 湖南农业大学动物科学技术学院, 长沙 410128;
2. 湖南农业大学东方科技学院, 长沙 410128;
3. 湖南生物机电职业技术学院, 长沙 410127

李美君(1983—),女,湖南益阳人,硕士研究生,从事单胃动物营养及生理的研究。E-mail: 601619178@qq.com

收稿日期: 2011-07-14

网络出版日期: 2012-01-13

基金资助

湖南省高等学校科学研究重点项目(08A030);大学生研究性学习和创新性实验计划重点项目(DFCXS201001)

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Effects of Lactoferrin Supplementation on Growth Performance, Intestinal Microflora and Mucosal Morphology of Early Weaned Piglets

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1. College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China;
2. College of Orient Science and Technology, Hunan Agricultural University, Changsha 410128, China;
3. College of Hunan Biological and Electromechanical Polytechnic, Changsha 410127, China

Received date: 2011-07-14

Online published: 2012-01-13

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摘要

本试验旨在研究在饲粮中添加乳铁蛋白(lactoferrin,LF)对断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响。试验选用96头体重相近的21日龄"杜×长×大"三元杂交断奶仔猪,随机分为4组,分别饲喂基础饲粮(对照组)、基础饲粮+250 mg/kg乳铁蛋白、基础饲粮+500 mg/kg乳铁蛋白和基础饲粮+750 mg/kg乳铁蛋白,每组4个重复,每个重复6头仔猪。正试期21 d。结果表明,与对照组和750 mg/kg乳铁蛋白组相比,饲粮中添加250和500 mg/kg乳铁蛋白可显著提高仔猪平均日增重(P<0.05);与对照组相比,不同水平乳铁蛋白可显著降低盲肠中大肠杆菌数量(P<0.05),其中添加500 mg/kg乳铁蛋白显著提高了盲肠、结肠中乳酸杆菌的数量(P<0.05),显著降低了结肠中大肠杆菌数量(P<0.05);添加250和500 mg/kg乳铁蛋白极显著提高了十二指肠、空肠、回肠的绒毛高度/隐窝深度比值(P<0.01)。结果提示,饲粮中添加乳铁蛋白可刺激肠道有益菌生长、降低有害菌增殖,从而改善肠道功能,具有提高仔猪生长性能的作用,本试验条件下乳铁蛋白的适宜添加量为250 mg/kg。

关键词： 乳铁蛋白; 生长性能; 肠道菌群; 肠黏膜形态; 断奶仔猪

本文引用格式

李美君, 方成堃, 张凯, 李运虎, 方热军 . 饲粮中添加乳铁蛋白对早期断奶仔猪生长性能、肠道菌群及肠黏膜形态的影响[J]. 动物营养学报, 2012 , 24(1) : 111 -116 . DOI: 10.3969/j.issn.1006-267x.2012.01.016

Abstract

This experiment was conducted to investigate the effects of lactoferrin(LF)on growth performance, intestinal microflora and mucosal morphology in early weaned piglets. Ninety-six piglets weaned at 21 days of age were randomly divided into four groups with four replicates in every group and 6 piglets in every replicate. The four groups were basal diet group, basal diet+250 mg/kg LF group, basal diet+500 mg/kg LF group and basal diet+750 mg/kg LF group, respectively. The experiment lasted for 21 days. The results showed that compared with the control and 750 mg/kg LF group, diets supplemented with 250 and 500 mg/kg LF could significantly improve average daily gain(P<0.05);compared with the control group,LF could reduce the sum of Escherichia coli in caecum(P<0.05). The supplementation of 500 mg/kg LF was significantly increased Lactobacillus number in caecum and colon(P<0.05)as well as significantly reduced Escherichia coli number in colon(P<0.05).The supplementation of 250 and 500 mg/kg LF were extremely significantly increased villus height to crypt depth ratio in duodenum, jejunum and ileum(P<0.01). The results indicate that supplementation of LF can improve the growth performance and regulate intestinal microflora as well as mucosal morphology. The optimum supplemental level of LF is 250 mg/kg under this experimental condition.

Key words： lactoferrin; growth performance; intestinal microflora; intestinal mucosal morphology; weaned piglets

参考文献

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[5] 伍喜林.乳铁蛋白对隔离早期断奶仔猪营养生理效应的研究[D].博士学位论文.雅安:四川农业大学,2004.

[6] TERAGUCHI S, SHIN K, OZAWA K, et al.Bacteriostatic effect of orally administered bovine lactoferrin on proliferation of Clostridium species in the gut of mice fed bovine milk[J]. Applied and Environmental Microbiology, 1995, 61(2):501-506.

[7] 刘红云,富童淡.乳铁蛋白生理功能研究现状[J].上海畜牧兽医通讯,2004(3):2-4.

[8] HELLWEG P, KRAMMER-LUKAS S, STRASSER A, et al.Effects of bovine lactoferrin on the immune system and the intestinal microflora of adult dogs[J]. Archives of Animal Nutrition, 2008, 62(2):152-161.

[9] WANG Y Z, SHAN T Z, XU Z R, et al. Effects of the lactoferrin (LF) on the growth performance, intestinal microflora and morphology of weanling pigs[J]. Animal Feed Science and Technology, 2007, 135(3/4):263-272.

[10] BULLEN J J. Iron-binding protein in milk and resistance to Escherichia coli infection in infants[J]. Proceedings of the Royal Society of Medicine, 1972, 65(12):1086.

[11] KAWAKAMI H, LÖNNERDAL B. Isolation and function of a receptor for human lactoferrin in human fetal intestinal brush-border membranes[J]. The American Journal of Physiology, 1991, 261(5):G841-G846.

[12] DI MARIO F, ARAGONA G, DALBÓ N, et al. Bovine lactoferrin for Helicobacter pylori eradication: an open, randomized, multicentre study[J]. Alimentary Pharmacology & Therapeutics, 2006, 23(8):1235-1240.

[13] 张祥.不同乳铁蛋白含量的代乳粉对犊牛生长发育的影响[D].硕士学位论文.扬州:扬州大学,2007.

[14] HUMPHREY B D, HUANG N, KLASSING K C. Rice expressing lactoferrin and lysozyme has antibiotic-like properties when fed to chicks[J]. The Journal of Nutrition, 2002, 132(6):1214-1218.

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