动物营养学报 >

2012 , Vol. 24 >Issue 1: 124 - 130

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.01.018

饲料营养

饲粮添加共轭亚油酸对肉仔鸡肠道黏膜免疫反应的影响

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  • 1. 河南省高校动物营养与饲料工程技术研究中心, 郑州 450011;
    2. 郑州牧业工程高等专科学校, 郑州 450011
刘永祥(1969—),男,山东成武人,博士,主要从事单胃动物营养研究。E-mail: yxliu225@163.com

收稿日期: 2011-08-08

  网络出版日期: 2012-01-13

基金资助

河南省科技厅重点科技公关资助项目(082102110123);郑州牧专博士基金项目(2007DSR003)

收起

Dietary Conjugated Linoleic Acid Affects Intestinal Mucosal Immunity Response of Broiler Chickens

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  • 1. Engineering Technology Research Center of Animal Nutrition and Feed, Henan University, Zhengzhou 450011, China;
    2. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China

Received date: 2011-08-08

  Online published: 2012-01-13

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摘要

本试验旨在研究饲粮添加共轭亚油酸(conjugated linoleic acid,CLA)对肉仔鸡肠道黏膜免疫反应的影响。试验选用72只1日龄健康、体重相近的爱拔益加(AA)雄性肉仔鸡,随机分配到对照组和CLA组,分别饲喂基础饲粮和在基础饲粮中添加1% CLA的饲粮,每组6个重复,每个重复6只鸡。21日龄屠宰取样。结果表明:CLA组肉仔鸡空肠食糜分泌型免疫球蛋白A(secretory immunoglobulin A,SIgA)含量(P<0.01)和空肠SIgA基因mRNA表达水平(P<0.01)均高于对照组;饲粮添加CLA显著提高肉仔鸡空肠过氧化物酶体增殖物激活受体γ(PPARγ)基因mRNA表达水平(P<0.01),但转化生长因子β4(transforming growth factor-β4,TGFβ4)基因mRNA表达水平在2组之间没有显著差异(P>0.05);CLA组肉仔鸡Peyer氏结CD8+ T细胞亚群百分数(P<0.01)和淋巴细胞转化率(P<0.05)均显著高于对照组。由此可见,饲粮添加1% CLA提高了21日龄肉仔鸡空肠SIgA水平,激活PPARγ可能是CLA调节SIgA表达的途径之一。饲粮添加1% CLA影响21日龄肉仔鸡空肠黏膜细胞免疫功能。

关键词： 共轭亚油酸; 肉仔鸡; 肠道黏膜免疫

本文引用格式

刘永祥, 刘艳丽, 徐秋良, 姜东风, 杨建平 . 饲粮添加共轭亚油酸对肉仔鸡肠道黏膜免疫反应的影响[J]. 动物营养学报, 2012 , 24(1) : 124 -130 . DOI: 10.3969/j.issn.1006-267x.2012.01.018

Abstract

This experiment was conducted to investigate the effects of dietary conjugated linoleic acid (CLA) on the intestinal mucosal immunity response of broiler chickens. Seventy-two 1-day-old male Abor Acre (AA) broiler chickens were randomly allocated into two groups and fed a basal diet or a diet supplemented with 1% CLA, and each group contained six replicates with 6 chicks each and 6 chicks in each replicate. The brolier chickens were slaughtered at 21 days of age and samples were collected. Chicks fed the CLA supplementation diet had significantly higher jejunal chyme SIgA content and jejunum SIgA gene mRNA expression compared with those fed the basal diet (P<0.01). Jejunal peroxisome proliferator-activated receptor γ (PPARγ) gene mRNA expression was significantly enhanced in chicks fed the CLA supplementation diet than that in chicks fed the basal diet (P<0.01), but the transforming growth factor-β4 (TGFβ4) gene mRNA expression was not significantly different between them (P>0.05). Dietary CLA increased the percentages of CD8+ T cell subsets of Peyer's patch (P<0.01) and lymphocyte transformation rate (P<0.05). Dietary 1% CLA increases jejunum SIgA secretion, at least in part, through activating the PPARγ transcription. Dietary 1% CLA infulences the jejunal mucosal cell immunity response of broiler chickens.

Key words： CLA; broiler chickens; intestinal mucosal immunity

参考文献

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