本试验采用鱼粉、酪蛋白和玉米蛋白粉为蛋白质源,配制蛋白质水平分别为35%、40%、45%和50%的4种等能饲料,以确定乌苏里拟鲿稚鱼饲料中蛋白质的适宜水平。试验选用乌苏里拟鲿稚鱼[初始体重为(3.40±0.01)g]300尾,随机分为4组,每组设3个重复,每个重复25尾鱼。试验期为8周。结果表明:45%组乌苏里拟鲿稚鱼的增重率和特定生长率显著高于35%和40%组(P<0.05),但与50%组差异不显著(P>0.05);而饲料系数则表现出相反的变化趋势。随着饲料蛋白质水平从35%升高到50%,乌苏里拟鲿稚鱼的蛋白质效率显著降低(P<0.05)。饲料蛋白质水平对乌苏里拟鲿稚鱼全鱼和肌肉水分以及全鱼灰分含量无显著影响(P>0.05)。45%组乌苏里拟鲿稚鱼的全鱼和肌肉粗蛋白质含量显著高于35%和40%组(P<0.05)。随着饲料蛋白质水平的升高,乌苏里拟鲿稚鱼的全鱼粗脂肪含量呈先增加后降低趋势,而肌肉粗脂肪和灰分含量则呈降低趋势。各组乌苏里拟鲿稚鱼的肥满度、肝胰指数和内脏指数无显著差异(P>0.05)。在本试验条件下,以增重率和饲料系数为指标,乌苏里拟鲿稚鱼饲料中蛋白质的适宜水平为45%。
This study was conducted to determine the optimum dietary protein level of juvenile Pseudobagrus ussuriensis. Four isoenergetic diets were formulated with fish meal, casein and corn gluten meal as protein sources containing graded levels of protein: 35%, 40%, 45% and 50%, respectively. A total of 300 juvenile Pseudobagrus ussuriensis with an initial body weight of (3.40±0.01) g were randomly divided into 4 groups with 3 replicates per group and 25 fish per replicate. The experiment lasted for 8 weeks. The results showed as follows: the weight gain rate and specific growth rate of juvenile Pseudobagrus ussuriensis in 45% group were significantly higher than those in 30% and 40% groups (P<0.05), but there were no significant differences between the 45% and 50% groups (P>0.05); however, the feed conversion ratio showed an opposite trend. With increasing dietary protein level from 35% to 50%, the protein efficiency ratio was significantly decreased (P<0.05). The moisture and ash contents in whole fish and moisture content in muscle of juvenile Pseudobagrus ussuriensis were not significantly affected by dietary protein level (P>0.05). The crude protein content in whole fish and muscle of juvenile Pseudobagrus ussuriensis in 45% group was significantly higher than that in 30% and 40% groups (P<0.05). With increasing dietary protein level, the crude lipid content in whole fish of juvenile Pseudobagrus ussuriensis was firstly increased and then decreased, while the crude lipid and ash contents in muscle were decreased. No significant differences were found in condition factor, hepaticsomatic index and viscerosomatic index of juvenile Pseudobagrus ussuriensis among all groups (P>0.05). In this experiment, based on the indices of weight gain rate and feed conversion ratio, the optimum dietary protein level of juvenile Pseudobagrus ussuriensis is 45%.
[1] 张觉民.黑龙江省鱼类志[M].哈尔滨:黑龙江科学技术出版社,1995:205-207.
[2] 尹洪滨,孙中武,于波.乌苏里拟鲿(Pseudobagrus ussuriensis)精子的超微结构[J].广东海洋大学学报,2009,29(6):28-31.
[3] 潘伟志,王鹏,赵春刚,等.乌苏里拟鲿人工繁育技术及开发利用——乌苏里拟鲿繁殖生物学及人工催产初步研究[J].水产学杂志,2001,14(2):1-3.
[4] 潘伟志,尹洪滨,孙中武,等.乌苏里拟鲿(Pseudobagrus ussuriensis)同工酶分析[J].东北林业大学学报,2006,34(6):66-69.
[5] 李爱杰.水产动物营养与饲料学[M].北京:中国农业出版社,1996.
[6] 林小植,谢小军,罗毅平.中华倒刺鲃幼鱼饲料蛋白质需求量的研究[J].水生生物学报,2009,33(4):674-681.
[7] 王鹏,赵春刚,叶继丹,等.乌苏里拟鲿人工繁育技术及开发利用——乌苏里拟鲿仔幼鱼食性与生长的初步研究[J].水产学杂志,2001,14(2):4-6.
[8] 吴建开,朱邦科,雍文岳,等.黄颡鱼幼鱼对蛋白质和能量的需要量[C]//中国畜牧兽医学会动物营养学分会第九届学术研讨会论文集.北京:中国农业科学技术出版社,2004.
[9] 孙丽,郭贵良,闫先春,等.东北六须鲶对蛋白质的最适需求量[J].饲料工业,2009,30(12):33-35.
[10] 张文兵,谢小军,付世杰,等.南方鲇的营养学研究:饲料的最适蛋白质含量[J].水生生物学报,2000,24(6):603-609.
[11] 蒋蓉,宋学宏,叶元土,等.黄颡鱼饲料中适宜的蛋白质含量和能量蛋白比[J].大连水产学院学报,2004,19(4):252-257.
[12] 王武,石张东,甘炼.江黄颡鱼幼鱼最适蛋白质需求量的研究[J].上海水产大学学报,2003,12(2):185-188.
[13] KIM S S, LEE K J. Dietary protein requirement of juvenile tiger puffer (Takifugu rubripes)[J]. Aquaculture, 2009, 287:219-222.
[14] LEE S M, KIM K D, PARK H G, et al. Protein requirement of juvenile Manchurian trout Brachymystax lenok[J]. Fisheries Science, 2001, 67:46-51.
[15] 邵庆均,苏小凤,许梓荣,等.饲料蛋白水平对宝石鲈生长和体组成影响研究[J].水生生物学报,2004,28(4):367-373.
[16] SAMANTARAY K, MOHANTY S S. Interactions of dietary levels of protein and energy on fingerling snakehead, Channa striata[J]. Aquaculture, 1997, 156:245-253.
[17] KHAN M S, ANG K J, AMBAK M A, et al. Optimum dietary protein requirement of a Malaysian freshwater catfish, Mystus nemurus[J]. Aquaculture, 1993, 112:227-235.
[18] TIBBETTS S M, LALL S P, ANDERSON D M. Dietary protein requirement of juvenile American eel (Anguilla rostrata) fed practical diets[J]. Aquaculture, 2000, 186:145-155.
[19] 张文兵,付世建,曹振东,等.不同配合饲料对南方鲇幼鱼鱼体组成的影响[J].西南师范大学学报:自然科学版,1999,24(3):325-331.
[20] PAGE J W. Interaction of dietary levels of protein and energy on channel catfish (Ictalurus punctatus)[J]. The Journal of Nutrition, 1973, 103(9):1339-1346.
[21] ARZEL J, METAILLER R, KERLEGUER C, et al. The protein requirement of brown trout (Salmo trutta) fry[J]. Aquaculture, 1995, 130:67-78.
[22] 刘永坚,刘栋辉,田丽霞,等.蛋白质和能量水平对红姑鱼生长和鱼体组成的影响[J].水产学报,2002,26(3):242-246.
[23] ZEITLER M H, KIRCHGESSNER M, SCHWARZ F J. Effects of different protein and energy supplies on carcass composition of carp (Cyprinus carpio L.)[J]. Aquaculture, 1984, 36:37-48.
[24] SHEARER K D. Factors affecting the proximate composition of cultured fishes with emphasis on salmonids[J]. Aquaculture, 1994, 119:63-88.
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