铁过量或缺乏对新生仔猪血清生化指标及肝脏 hepcidin mRNA表达量的影响

刘庆华; 杨建平; 刘延贺; 朱宽佑; 李梦云

doi:10.3969/j.issn.1006-267x.2012.05.008

动物营养学报 >

2012 >Issue 5: 845 - 851

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.008

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铁过量或缺乏对新生仔猪血清生化指标及肝脏 hepcidin mRNA表达量的影响

刘庆华 ,
杨建平 ,
刘延贺 ,
朱宽佑 ,
李梦云

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1. 郑州牧业工程高等专科学校, 郑州 450011;
2. 河南省无公害饲料工程技术研究中心, 郑州 450011

收稿日期: 2011-11-12

网络出版日期: 2012-04-29

基金资助

河南省科技攻关项目(102102110113)

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Effects of Iron Overload or Deficiency on Serum Biochemical Indices and Liver Hepcidin mRNA Expression of Newborn Piglets

LIU Qinghua ,
YANG Jianping ,
LIU Yanhe ,
ZHU Kuanyou ,
LI Mengyun

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1. Zhengzhou College of Animal Husbandry and Engineering, Zhengzhou 450011, China;
2. Henan Pollution-Free Feed Engineering Technology Research Center, Zhengzhou 450011, China

Received date: 2011-11-12

Online published: 2012-04-29

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摘要

本试验旨在研究铁过量或缺乏对新生仔猪血清生化指标及肝脏hepcidin mRNA表达量的影响。挑选新出生的"杜长大"三元杂交仔猪15头,随机分为3组,即缺铁组、正常组和铁过量组,每组5个重复,每个重复1头猪。3和7日龄时,缺铁组分别注射1 mL生理盐水,正常组分别注射1 mL右旋糖酐铁(含铁150 mg),铁过量组分别注射3 mL右旋糖酐铁(含铁450 mg)。7日龄时,将所有仔猪全部处死,采集血清,并分离肝脏和脾脏,以测定血清生化指标、机体铁含量和肝脏hepcidin mRNA表达量。结果表明:肝脏、脾脏和血清中铁的含量均随着注射铁量的增加而显著或极显著增加(P<0.05或P<0.01)。与正常组相比,铁过量组血清中血红蛋白、球蛋白、总蛋白、丙二醛含量以及谷胱甘肽过氧化物酶、过氧化物酶活性显著或极显著升高(P<0.05或P<0.01),超氧化物歧化酶活性显著降低(P<0.05);而缺铁组血清中血红蛋白、球蛋白、总蛋白、丙二醛含量以及谷胱甘肽过氧化物酶、过氧化氢酶、过氧化物酶活性则显著或极显著降低(P<0.05或P<0.01),超氧化物歧化酶活性显著升高(P<0.05)。与正常组相比,铁过量组仔猪肝脏中hepcidin mRNA表达量极显著升高(P<0.01),而缺铁组则极显著降低(P<0.01)。由此得出,铁过量或缺乏均会影响新生仔猪机体的免疫功能和抗氧化功能;铁过量可提高新生仔猪机体铁含量和肝脏中hepcidin mRNA表达量,铁缺乏则会降低新生仔猪机体铁含量和肝脏中hepcidin mRNA表达量。

关键词： 新生仔猪; 铁过量; 缺铁; 血清生化指标; hepcidin mRNA表达量

本文引用格式

刘庆华 , 杨建平 , 刘延贺 , 朱宽佑 , 李梦云 . 铁过量或缺乏对新生仔猪血清生化指标及肝脏 hepcidin mRNA表达量的影响[J]. 动物营养学报, 2012 , (5) : 845 -851 . DOI: 10.3969/j.issn.1006-267x.2012.05.008

Abstract

This experiment was conducted to investigate the effects of iron overload or deficiency on serum biochemical indices and liver hepcidin mRNA expression of newborn piglets. A total of 15 cross-bred (Duroc×Landrace×Large white) neonatal piglets with an average body weight of (1.22±0.13) kg were randomly divided into 3 groups (iron-deficiency group, regular group and iron-overload group) with 5 replicates per group and 1 piglet per replicate. At 3 and 7 days of age, the piglets in the 3 groups were injected 1 mL physiological saline, 1 mL dextriferron (450 mg iron) and 3 mL dextriferron (450 mg iron), respectively. At 7 days of age, all piglets were killed, and blood, liver and spleen were collected to measure iron content, serum biochemical indices and liver hepcidin mRNA expression. The results showed as follows: iron contents in liver, spleen and serum were all significantly increased with injected Fe content increasing (P<0.05 or P<0.01). Compared with the regular group, the contents of hemoglobin, globulin, total protein and malondialdehyde and the activities of glutathione peroxidase and peroxidase in serum were significantly increased and the superoxide dismutase activity in serum was significantly decreased in iron-overload group (P<0.05 or P<0.01), while the contents of hemoglobin, globulin, total protein and malondialdehyde and the activities of glutathione peroxidase, catalase and peroxidase in serum were significantly decreased and the superoxide dismutase activity in serum was significantly increased in iron-deficiency group (P<0.05 or P<0.01). Compared with the regular group, liver hepcidin mRNA expression was significantly increased in iron-overload group (P<0.01) and significantly decreased in iron-deficiency group (P<0.01). In conclusion, iron overload or deficiency can affect the immune function and anti-oxidant function of newborn piglets; iron overload and deficiency can increase and decrease body’s iron content and liver hepcidin mRNA expression of newborn pigs, respectively.

Key words： newborn piglets; iron overload; iron deficiency; serum biochemical indices; hepcidin mRNA expression

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