精氨酸水平对奶牛乳腺上皮细胞体外生长及 κ-酪蛋白基因表达的影响

徐柏林; 王梦芝; 张兴夫; 哈斯; 王春艳; 敖长金; 王洪荣

doi:10.3969/j.issn.1006-267x.2012.05.009

动物营养学报 >

2012 >Issue 5: 852 - 858

DOI: https://doi.org/10.3969/j.issn.1006-267x.2012.05.009

分子营养

精氨酸水平对奶牛乳腺上皮细胞体外生长及 κ-酪蛋白基因表达的影响

徐柏林 ,
王梦芝 ,
张兴夫 ,
哈斯 ,
王春艳 ,
敖长金 ,
王洪荣

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1. 扬州大学动物科学与技术学院, 扬州 225009;
2. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
3. 内蒙古农业大学动物科学学院, 呼和浩特 010018

收稿日期: 2011-12-12

网络出版日期: 2012-04-29

基金资助

国家重点基础研究发展规划(973计划)项目(2011CB100803);动物营养学国家重点实验室开放课题(2004DA125184F1106)

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Arginine Levels Affect Growth and CSN3 Gene Expression of Dairy Cows Mammary Epithelial Cells in Vitro

XU Bolin ,
WANG Mengzhi ,
ZHANG Xingfu ,
HA Si ,
WANG Chunyan ,
AO Changjin ,
WANG Hongrong

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1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Science, Beijing 100193, China;
3. College of Animal Science, Inner Mongolia Agricultural University, Inner Mongolia 010018, China

Received date: 2011-12-12

Online published: 2012-04-29

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摘要

本试验旨在研究培养基中添加不同浓度的精氨酸(Arg)对乳腺上皮细胞体外增殖及κ-酪蛋白(CSN3)基因表达的影响。选用中国荷斯坦奶牛乳腺上皮细胞进行体外培养,以无Arg的培养基(0.00 mg/L)为对照(0组),试验培养基分别添加69.50(0.25组)、139.00(0.50组)、278.00(1.00组)、556.00(2.00组)、1 112.00(4.00组)和2 224.00 mg/L(8.00组)的精氨酸。结果表明:Arg能促进乳腺上皮细胞的增殖,24 h时,0.25~4.00组与0组相比均差异显著(P<0.05),8.00组与0组相比差异不显著(P>0.05);48和72 h时,各试验组与0组相比均差异显著(P<0.05)。Arg能促进CSN3基因的表达,各试验组与0组相比均差异显著(P<0.05),当Arg浓度为556.00 mg/L时,CSN3基因表达量最高。结果提示,Arg对乳腺上皮细胞增殖及CSN3基因表达均具有明显的促进作用,且在Arg浓度为69.50~1 112.00 mg/L时促细胞增殖效果较佳,在Arg浓度为556.00 mg/L时促CSN3基因表达作用最强。

关键词： 乳腺上皮细胞; 精氨酸; 细胞增殖; κ-酪蛋白基因; 基因表达

本文引用格式

徐柏林 , 王梦芝 , 张兴夫 , 哈斯 , 王春艳 , 敖长金 , 王洪荣 . 精氨酸水平对奶牛乳腺上皮细胞体外生长及 κ-酪蛋白基因表达的影响[J]. 动物营养学报, 2012 , (5) : 852 -858 . DOI: 10.3969/j.issn.1006-267x.2012.05.009

Abstract

This experiment was conducted to investigate effects of supplementation of arginine (Arg) in culture medium at different levels on proliferation and κ-casein (CSN3) gene expression of mammary epithelial cells (MECs) in vitro. MECs of Chinese Holstein dairy cow were used in this in vitro study. Supplementation levels of Arg were 0.00 (0 group, control group), 69.50 (0.25 group), 139.00 (0.50 group), 278.00 (1.00 group), 556.00 (2.00 group), 1 112.00 (4.00 group) and 2 224.00 mg/L (8.00 group), respectively. The results showed as follows: proliferation of MECs was promoted by Arg, after cultured for 24 h, compared with 0 group, promotion efficiencies of groups 0.25 to 4.00 were significantly higher (P<0.05), while that of 8.00 group was not significantly changed (P>0.05); after cultured for 48 and 72 h, there were significant differences in promotion efficiency between all experimental groups and the control group (P<0.05). Expression level of CSN3 gene was increased by Arg, CSN3 gene expression level of all experimental groups was significantly higher than that of 0 group (P<0.05), and the expression level was highest when the Arg concentration was 556.00 mg/L in culture medium. In conclusion, Arg has a stimulatory effect on proliferation and CSN3 gene expression of MECs. The stimulatory effect on cell proliferation is better when Arg concentration is from 69.50 to 1 112.00 mg/L in culture medium, and the stimulatory effect on CSN3 gene expression is the best when Arg concentration is 556.00 mg/L in culture medium.

Key words： mammary epithelial cell; arginine; cell proliferation; CSN3 gene; gene expression

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