本试验旨在研究8~12周龄肉兔缬氨酸(Val)和异亮氨酸(Ile)的需要量及其与赖氨酸(Lys)的适宜比例。试验采用4×3因子设计,4个Val水平分别为0.51%、0.65%、0.69%和0.73%,3个Ile水平分别为0.57%、0.61%、0.65%。选择平均体重(2 235.75±45.10) g可做种用的新西兰肉兔96只,随机分为12组,每组8个重复,每个重复1只兔。试验期28 d。结果表明:1)主效应分析表明,饲粮Val水平极显著影响肉兔平均日增重(ADG)(P<0.01),以0.69%Val组ADG最大;ADG随Ile水平的增加而增加,0.65%Ile组ADG最大。两因素方差分析表明,0.69%Val和0.61%Ile组ADG最大,但与0.69%Val和0.65%Ile、0.65%Val和0.65%Ile、0.73%Val和0.61%及0.73%Val和0.65%Ile组无显著差异(P>0.05)。2)各组平均日采食量(ADFI)和料重比(F/G)均无显著差异(P>0.05)。Val与Ile的互做对ADG、ADFI和F/G的影响不显著(P>0.05)。3)血清尿素氮含量以0.69%Val和0.61%Ile组最低,但各组差异不显著(P>0.05)。综合分析,以0.69%Val和0.61%Ile添加水平较适宜,其实测值与估计值的平均值分别为0.66%和0.56%;Lys、Val和Ile三者的比例为1.00:0.83:0.70。
This experiment was conducted to study the requirements of valin (Val) and isoleucine (Ile) and their appropriate ratio to lysine (Lys) for meat rabbit aged 8 to 12 weeks. A 4?3 factorial trial was adapted, and rabbits were fed the experimental dies with 0.51%, 0.65%, 0.69%, 0.73% Val, and 0.57%, 0.61%, 0.65% Ile, respectively. Ninety six breed New Zealand meat rabbits with an average body weight of (2 235.75?45.10) g were randomly divided into 12 groups with 8 replicates per group and 1 rabbit per replicate. The experiment lasted for 28 days. The results showed as follows: 1) the main effect analysis showed that dietary Val level had significant influence on average daily gain (ADG) of meat rabbits (P<0.01), and the maximum ADG was in 0.69% Val group. ADG was increased with the increasing of Val level, and the maximum ADG was in 0.69% group. Two factors variance analysis showed that the maximum ADG was in 0.69% Val and 0.61% Ile group, but there was no significant difference between that group and adjacent groups (Val 0.69% and Ile 0.65%, Val 0.65% and Ile 0.65%, Val 0.73% and Ile 0.61%) (P>0.05). 2) There were no significant differences in average daily feed Intake (ADFI) and feed to gain (F/G) among all the groups (P>0.05). The interaction of Val and Ile had no significant effects on ADFI and F/G (P>0.05). 3) There were no significant differences in serum urea nitrogen (UN) content (P>0.05), but the 0.69% Val and 0.61% Ile group showed the lowest serum UN content. In conclusion, the recommend requirements of Val and Ile for rabbit are 0.69% and 0.61%, and the average measured value and estimated value are 0.66% and 0.56%; the ratio of Lys:Val:Ile is 1: 0.83: 0.70.
[1] NRC.Nutrient requirements of rabbits[S].2th ed.Washington,D.C.:National Academy Press,1977.
[2] 张宏福.动物营养参数与饲养标准[M].2版.北京:中国农业出版社,2010:520-522.
[3] MOUGHAN P J,SCHULIZE W H,SMITH W C.Amino acid requirements of the growing meat rabbit.1.The amino acid composition of rabbit whole body tissue-a theoretical estimate of ideal amino acid balance[J].Animal Production,1988,47:297-301.
[4] 何瑞国.生长期实验兔日粮消化能、蛋白质、粗纤维适宜水平和氨基酸模式的研究[J]中国实验动物学报,2000(8):53-61.
[5] KLAUS J.A compendium of Rabbit production; appropriate for conditions in developing countries[M].Eschborn:GTZ,1985:224.
[6] 徐立德.家兔生产学[M].北京:中国农业出版社,1998:56-61.
[7] 南京农业大学畜牧系.兔营养需要研究论文选编[M].南京:南京农业大学出版社,1988.
[8] 杨正.现代养兔[M].北京:中国农业出版社,1999.
[9] MALMLOF K.Amino acid in farm animal nutrition metabolism partition and consequences of imbalance[J].Journal of Agriculture Research,1988,18:191-193.
[10] DEGUSSA.The amino acids composition of feedstuffs[M].Allendale, N.J.:Degussa Corporation,1990.
[11] NRC.Nutrient requirement of poultry[S].9th ed.Washington,D.C.:The National Academy Press,1994.
[12] NRC.Nutrient requirement of swine[S].10th ed.Washington,D.C.:The National Academy Press,1998.
[13] DE BLAS C J,MATEOS G G.Feed formulation[M].New York:CABI Publishing,1998:241-253.