本试验旨在探讨饲粮中添加丁酸梭菌对蛋用仔公鸡肠道菌群、形态结构及黏膜免疫相关细胞的影响。试验选择240只1日龄健康海兰褐蛋用仔公鸡,随机分为6组,每组4个重复,每个重复10只鸡,各组分别在基础饲粮中添加0(对照组)和150 mg/kg金霉素(抗生素组)以及250、500、750、1 000 mg/kg丁酸梭菌,试验期为42 d。结果表明:1)750 mg/kg丁酸梭菌组与1 000 mg/kg丁酸梭菌组的盲肠内大肠杆菌数与对照组和抗生素组相比显著降低(P<0.05),除250 mg/kg丁酸梭菌组外,其他各丁酸梭菌组盲肠内乳杆菌数量均显著高于对照组和抗生素组(P<0.05)。2)1 000mg/kg丁酸梭菌组小肠各肠段的绒毛高度显著高于对照组和抗生素组,绒毛长度/隐窝深度(V/C)值显著高于对照组(P<0.05),空肠的黏膜厚度显著高于其他各组(P<0.05)。3)1 000 mg/kg丁酸梭菌组的空肠与回肠上皮内淋巴细胞数量显著高于对照组(P<0.05),与抗生素组相比差异不显著(P>0.05),杯状细胞数量显著高于对照组与抗生素组(P<0.05)。1 000 mg/kg丁酸梭菌组的十二指肠和空肠的免疫球蛋白A(IgA)阳性物质有效数量显著高于对照组(P<0.05),与抗生素组相比差异不显著(P>0.05)。综上所述,丁酸梭菌能够促进生长发育,维持肠道健康,在本试验条件下,最适宜的添加水平为1 000 mg/kg。
This experiment was conducted to study the effects of Clostridium butyricum on intestinal microflora, morphology and mucosal immunity-associated cells in egg-laying male chicks. Two hundred and forty one-day-old Hy-Line egg-laying male chicks were randomly divided into 6 groups with 4 replicates in each group and 10 chicks in each replicate. The diets were supplemented with 0 (control group), 150 mg/kg aureomycin (antibiotic group), 250 (EG250), 500 (EG500), 750 (EG750) and 1 000 (EG1000) mg/kg Clostridium butyricum, respectively. The results showed as follows: 1) the number of E. coli in EG750 and EG1000 was significantly less than that of control group and antibiotic group (P<0.05), and except for EG250, the number of Lactobacillus in the other Clostridium butyricum groups was larger than that of control group and antibiotic group (P<0.05). 2)The villus height of small intestine of EG1000 was significantly higher than that of control group and antibiotic group, villus length/crypt depth (V/C) was significantly higher than that of control group, and mucosa thickness of jejunum was significantly higher than that of the other groups (P<0.05). 3) The number of intraepithelial lymphocyte of jejunum and ileum of EG1000 was significantly increased compared with that of control group (P<0.05), but was not significantly different from that in antibiotic group (P>0.05), the number of goblet cell of jejunum and ileum of EG1000 was significantly higher than that of control group and antibiotic group (P<0.05). The number of immunoglobulin A positive (IgA+) of duodenum and jejunum of EG1000 was significantly increased compared with that of control group (P<0.05), and not significantly different from that of antibiotic group (P>0.05). In conclusion, Clostridium butyricum can promote growth and maintain intestine health, and the optimum supplemental level is 1 000 mg/kg under the condition of this experiment.
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