饲料安全 Feed Security

饲粮中三聚氰胺对乳成分的影响及其在牛奶中的残留规律

  • 赵小伟 ,
  • 哈斯额尔敦 ,
  • 王加启 ,
  • 卜登攀 ,
  • 魏宏阳 ,
  • 周凌云 ,
  • 王俊 ,
  • 许晓敏
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  • 1. 甘肃农业大学动物科学技术学院, 兰州 730070;
    2. 中国农业科学院北京畜牧兽医研究所反刍动物营养研究室, 北京 100193;
    3. 农业部奶及奶制品质量监督检验测试中心(北京), 北京 100193

收稿日期: 2012-05-01

  网络出版日期: 2012-11-01

基金资助

现代农业产业技术体系专项资金资助(nycytx-04-01)

Effects of Dietary Melamine on Milk Composition and Its Regularity of Deposition in Milk

  • ZHAO Xiaowei ,
  • KHAS Erdene ,
  • WANG Jiaqi ,
  • BU Dengpan ,
  • WEI Hongyang ,
  • ZHOU Lingyun ,
  • WANG Jun ,
  • XU Xiaomin
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  • 1. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
    2. State Key Laboratory of Ruminant Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
    3. MOA-Milk and Dairy Product Inspection Center (Beijing), Beijing 100193, China

Received date: 2012-05-01

  Online published: 2012-11-01

摘要

本试验旨在研究饲粮中三聚氰胺对泌乳奶牛干物质采食量、产奶量和乳成分的影响,并探讨饲粮中三聚氰胺在牛奶中的残留规律。试验选择9头泌乳中期 的中国荷斯坦奶牛,随机分成3组,分别饲喂添加0.1%(MEL1组)、0.5%(MEL2组)和1.0%(MEL3组)三聚氰胺的饲粮(干物质基础)。试验期28 d。结果表明,饲粮添加三聚氰胺对奶牛干物质采食量、产奶量、乳脂、乳蛋白和乳糖含量无显著影响(P>0.05);但乳中尿素含量随着三聚氰胺添加量的增加显著升高(P<0.05)。乳中三聚氰胺含量随添加量的增加而显著增加(P<0.05),在MEL1、MEL2和MEL3这3个组的含量分别为5.48、28.32和51.96 mg/L,乳中三聚氰胺产量分别为66.00、420.05和745.20 mg/d。饲粮中三聚氰胺向乳中转化的效率在3个组间无显著差异(P>0.05),平均转化率为(0.51±0.10)%。在停喂三聚氰胺后牛奶中三聚氰胺含量迅速降低,在停喂后第3天三聚氰胺残留量均低于0.05 mg/L。综合本试验结果可知,泌乳奶牛日粮添加三聚氰胺可显著提高牛奶中三聚氰胺残留量,但对奶牛干物质采食量、产奶量和乳成分无显著影响。

关键词: 三聚氰胺; 牛奶; 残留; 奶牛

本文引用格式

赵小伟 , 哈斯额尔敦 , 王加启 , 卜登攀 , 魏宏阳 , 周凌云 , 王俊 , 许晓敏 . 饲粮中三聚氰胺对乳成分的影响及其在牛奶中的残留规律[J]. 动物营养学报, 2012 , (11) : 2222 -2229 . DOI: 10.3969/j.issn.1006-267x.2012.11.021

Abstract

The objective of this study was to determine the effects of dietary melamine on dry matter intake, milk yield and milk composition of lactating dairy cows, and to explored the regularity of deposition of melamine in milk. Nine multiparous Holstein cows in middle lactation [(160?21)d] were randomly allotted into 3 groups. Cows in the 3 groups were fed experimental diets supplemented with 0.1% (MEL1 group), 0.5% (MEL2 group) and 1.0% (MEL3 group) melamine (DM basis), respectively. The experiment lasted for 28 days. The results showed that dietary melamine had no significant influence on dry matter intake, milk yield, milk fat, milk protein and milk lactose of dairy cows (P>0.05), however, the urea content in milk was increased significantly with the increasing of dietary melamine (P<0.05). The melamine content in milk was increased significantly with the increasing of dietary melamine (P<0.05). The melamine contents in milk of MEL1, MEL2 and MEL3 groups were 5.48, 28.32 and 51.96 mg/L, and the melamine yields in milk of MEL1, MEL2 and MEL3 groups were 66.00, 420.05 and 745.20 mg/d, respectively. There was no significant difference in dietary melamine transfer rate in milk among all groups (P>0.05), and the average transfer rate was (0.51?0.1)%. The melamine residual were decreased rapidly after removing the melamine from diet, the residual level of melamine in milk was drop below 0.05 mg/L after the removed the melamine from diet on day 3. In conclusion, dietary melamine can significantly increase the residual level of melamine in milk, but has no significant influence on dry matter intake, milk yield and milk composition.

Key words: melamine; milk; residues; dairy cow

参考文献

[1] USFDA.Melamine pet food recall of 2007 [EB/OL].2010-11-29 [2012-03-26].http://www.fda.gov/oc/opacom/hottopics/petfood.html.

[2] USFDA.Interim melamine and analogues safety/risk assessment [EB/OL].2007-05-25 [2012-03-26].http://www.cfsan.fda.gov/~dms/melamra.html.

[3] NEWTON G L,UTLEY P R.Melamine as a dietary nitrogen source for ruminants[J].Journal of Animal Science,1978,47(6):1338-1344.

[4] BROOME A W.The use of non-protein nitrogen in animal feeds[M].London:J & A Churchill Ltd.,1968.

[5] 陈杰.家畜生理学[M].4版.北京:中国农业出版社,2003:425-439.

[6] CRUYWAGEN C W,STANDER M A,ADONIS M,et al.Hot topic:pathway confirmed for the transmission of melamine from feed to cow’s milk[J].Journal of Dairy Science,2009,92(4):2046-2050.

[7] BURNS K.Recall shines spotlight on pet foods[J].Journal of the American Veterinary Medical Association,2007,230(9),1285-1286.

[8] BURNS K.Events leading to the major recall of pet foods[J].Journal of the American Veterinary Medical Association,2007,230(11),1600-1620.

[9] 中华人民共和国农业部.NY/T 34-2004奶牛营养需要[S].北京:中国标准出版社,2004.

[10] ISO.Milk and milk products-Guidance on sampling (ISO 707:2008)[S]. [S.l.]:International Organization for Standardization,2008.

[11] 王加启,于建国.饲料分析与检验[M].北京:中国剂量出版社,2004.

[12] VAN SOEST P J,ROBERTSON J B,LEWIS B A.Methods for dietary fiber,neutral detergent fiber,and nonstarch polysaccharides in relation to animal nutrition[J].Journal of Dairy Science,1991,74(10):3583-3597.

[13] 中华人民共和国农业部.NY/T 1372-2007饲料中三聚氰胺的测定[S].北京:中国标准出版社,2007.

[14] USFDA.Updated FCC developmental melamine quantitation(HPLC-UV) [EB/OL].2007-04-02 [2012-03-26].http://www.fda.gov/cvm/melamine04022007.html.

[15] 中华人民共和国国家质量监督检验检疫总局.GB/T 22388-2008原料乳与乳制品中三聚氰胺检测方法[S].北京:中国标准出版社,2008.

[16] MELNICK R L,BOORMAN G A,HASEMAN J K,et al.Urolithiasis and bladder carcinogenicity of melamine in rodents [J].Toxicology and Applied Pharmacology,1984,72(2):292-303.

[17] OECD.OECD SIDS analysis UNEP publications:melamine[M]. [S.l.]:[s.n.],2002.

[18] CLARK R.Melamine crystalluria in sheep[J].Journal of the South African Veterinary Medical Association 1966;37:349-351.

[19] MACKENZIE H I.Melamine for sheep[J].Journal of the South African Veterinary Medical Association,1966;37:153-157.

[20] CLARK R,BARRATT E L,KELLERMAN J H.A comparison between nitrogen retention from urea,biuret,triuret and cyanuric acid by sheep on low protein roughage diet[J].Journal of the South African Veterinary Medical Association,1965,36:79-80.

[21] HATFIELD E E,GARRIGUS U S,FORBES R M,et al.Biuret-a source of NPN for ruminants[J]. Journal of Animal Science,1959,18:1208-1219.

[22] BAYNES R E,SMITH G,MASON S E,et al.Pharmacokinetics of melamine in pigs following intravenous administration [J].Food and Chemical Toxicology,2008,46(3):1196-1200.

[23] 林祥梅,王建峰,贾广乐,等.三聚氰胺的毒性研究[J].毒理学杂志,2008,22:216-218

[24] REIMSCHUESSEL R,GIESEKER C M,MILLER R A,et al.Evaluation of the renal effects of experimental feeding of melamine and cyanuric acid to fish and pigs[J].American Journal of Veterinary Research,2008,69(9):1217-1228.

[25] BROWN C A,JEONG K S,POPPENGA R H.Outbreaks of renal failure associated with melamine and cyanuric acid in dogs and cats in 2004 and 2007[J].Journal of Veterinary Diagnostic Investigation,2007,19:525-531.

[26] SATTER L D,ROFFLER R E.Nitrogen requirement and utilization in dairy cattle[J].Journal of Dairy Science,1975,58:1219.

[27] KAUFMAN A J,STPIERRE N R.The relationship of milk urea nitrogen to urine nitrogen excretion in Holstein and jersey cows[J].Journal of Dairy Science,2001,84:2284-2294.

[28] JONKER J S,KOHN R A,ERDMAN H A.Using milk urea nitrogen to predict nitrogen and utilization efficiency in lactating dairy cows[J].Journal of Dairy Science,1998,81:1681-2692.
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