刺五加多糖对脂多糖免疫应激断奶仔猪生长性能和血液生理生化指标的影响

韩杰; 边连全; 张一然; 刘显军; 张飞

doi:10.3969/j.issn.1006-267x.2013.05.021

动物营养学报 >

2013 , Vol. 25 >Issue 5: 1054 - 1061

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.05.021

饲料营养 Feed Science and Technology

刺五加多糖对脂多糖免疫应激断奶仔猪生长性能和血液生理生化指标的影响

韩杰 ,
边连全 ,
张一然 ,
刘显军 ,
张飞

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1. 沈阳农业大学畜牧兽医学院,沈阳 110866;
2. 洛阳师范学院生命科学系,洛阳 471022;
3. 沈阳农业大学科研种猪场,沈阳 110866

收稿日期: 2012-11-28

网络出版日期: 2013-04-12

基金资助

国家"十二五"科技支撑计划项目(2011BAD28B01- 02);科技部科技成果转化项目(2009GB2B000068)

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Effects of Acanthopanax senticosus Polysaccharide on Growth Performance and Blood Physiology and Biochemistry Indexes of Weaner Piglets Challenged with Lipopolysaccharide

HAN Jie ,
BIAN Lianquan ,
ZHANG Yiran ,
LIU Xianjun ,
ZHANG Fei

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1. College of Animal Husbandry and Veterinary, Shenyang Agricultural University, Shenyang 110866, China;
2. Department of Life Science, Luoyang Normal University, Luoyang 471022, China;
3. Breeding Pigs Field for Scientific Research, Shenyang Agricultural University, Shenyang 110866, China

Received date: 2012-11-28

Online published: 2013-04-12

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摘要

本试验旨在探讨饲粮中添加刺五加多糖(Acanthopanax senticosus polysaccharide,ASPS)对脂多糖(LPS)免疫应激断奶仔猪生长性能和血液生理生化指标的影响。试验采用2×2两因素设计,即饲粮处理(添加0或800 mg/kg ASPS)和免疫应激处理(注射LPS或生理盐水)。将64头(28±3)日龄、平均体重为(7.22±0.46) kg的"杜×长×大"三元杂交断奶仔猪随机分为4个处理,其中处理1和2饲喂基础饲粮(添加0 mg/kg ASPS),处理3和4饲喂试验饲粮(添加800 mg/kg ASPS),试验第14和21天,处理2和4仔猪腹腔注射100 μg/kg BW LPS,处理1和3仔猪腹腔注射等量生理盐水。注射后3 h采血,测定血液生理生化指标。试验期21 d。结果表明:试验1～14 d,饲喂ASPS对未注射LPS的仔猪生长性能无显著影响(P>0.05);试验15～21 d,饲喂ASPS显著增加仔猪的平均日增重(ADG)和平均日采食量(ADFI)(P<0.05),且饲喂ASPS使注射LPS的仔猪ADG和ADFI显著增加(P<0.05),但对注射生理盐水的仔猪无显著影响(P>0.05)。饲喂ASPS对仔猪ADG的影响与LPS刺激存在显著互作关系(P<0.05)。试验第14天,饲喂ASPS显著提高了仔猪外周血淋巴细胞数量(P<0.05),对外周血淋巴细胞数量的影响与LPS刺激存在显著的互作关系(P<0.05),且饲喂ASPS能显著增加注射LPS的仔猪外周血淋巴细胞数量(P<0.05),但对注射生理盐水的仔猪无显著影响(P>0.05)。试验第14和21天,饲喂ASPS显著降低了仔猪血浆α-酸性糖蛋白(α-AGP)、葡萄糖、前列腺素E₂(PGE₂)含量(P<0.05),显著提高了血浆白细胞介素-2(IL-2)含量(P<0.05),且其对α-AGP、IL-2、PGE₂含量的影响与LPS刺激存在显著的互作关系(P<0.05),饲喂ASPS能显著降低注射LPS的仔猪血浆α-AGP、PGE₂含量(P<0.05),但对注射生理盐水的仔猪无显著影响(P>0.05)。由此可见,饲喂ASPS对非免疫应激断奶仔猪的生长性能无影响,但可以缓解免疫应激断奶仔猪的生长抑制,ASPS缓解免疫应激断奶仔猪生长抑制与降低其血浆α-AGP和PGE₂含量,提高外周血淋巴细胞数量和血浆IL-2含量有关。

关键词： 刺五加多糖; 断奶仔猪; 脂多糖; 生长性能; 免疫应激

本文引用格式

韩杰 , 边连全 , 张一然 , 刘显军 , 张飞 . 刺五加多糖对脂多糖免疫应激断奶仔猪生长性能和血液生理生化指标的影响[J]. 动物营养学报, 2013 , 25(5) : 1054 -1061 . DOI: 10.3969/j.issn.1006-267x.2013.05.021

Abstract

This experiment was conducted to evaluate the effects of dietary Acanthopanax senticosus polysaccharide (ASPS) on growth performance and blood physiology and biochemistry indexes of weaner piglets challenged with lipopolysaccharide (LPS). The experiment was a 2×2 factorial design with two factors: diet (supplementation with ASPS or not) and immunological challenge (LPS or normal saline injection). A total of 64 crossbred barrows (Duroc×Large White×Landrace), aged (28±3) days, with an initial weight of (7.22±0.46) kg were randomly allotted to 4 treatments, treatments 1 and 2 were fed a basal diet with 0 mg/kg ASPS, and treatments 3 and 4 were fed the basal diet with 800 mg/kg ASPS. On days 14 and 21, pigs in treatments 2 and 4 were given an intraperitoneal injection with 100 μg/kg BW of LPS, and pigs in the other treatments were given equivalent amount of normal saline. Blood samples were obtained at 3 h after injection to analyze blood physiology and biochemistry parameters. The experiment lasted for 21 days. The results showed as follows: on days 1 to 14, ASPS supplementation had no significant effect on growth performance of piglets without LPS challenge (P>0.05). On days 15 to 21, ASPS supplementation significantly increased average daily gain (ADG) and average daily feed intake (ADFI) of piglets (P<0.05), and there was a significant interaction between ASPS supplementation and LPS challenge on the effect of ADG (P<0.05). ASPS supplementation significantly increased ADG and ADFI in LPS challenged piglets (P<0.05), but did not in normal saline-injected piglets (P>0.05). On day 14, ASPS supplementation significantly increased the number of peripheral blood lymphocytes (P<0.05), and it had significant interaction with LPS challenge (P<0.05). ASPS supplementation significantly increased the number of peripheral blood lymphocytes in LPS challenged piglets (P<0.05), but did not in normal saline-injected piglets (P>0.05). On days 14 and 21, ASPS supplementation significantly reduced the plasma contents of α-acid glycoprotein (α-AGP), glucose and prostaglandin (PGE₂) (P<0.05) as well as significantly increased the content of interleukin-2 (IL-2) of piglets (P<0.05). ASPS supplementation significantly reduced the plasma contents of α-AGP, glucose and PGE₂ in LPS challenged piglets (P<0.05), but did not in normal saline-injected piglets (P>0.05). The results indicate that ASPS supplementation can alleviate growth-depression of immunological challenge weaner piglets by depressed contents of α-AGP, PGE₂ as well as increase IL-2 content in plasma and the number of peripheral blood lymphocytes, but has no effect on growth performance in normal weaner piglets.

Key words： Acanthopanax senticosus polysaccharide; weaner piglets; lipopolysaccharide; growth performance; immune challenge

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