本试验旨在研究红芪粗多糖(CHPS)对细菌脂多糖(LPS)刺激断奶仔猪生长性能、血清生化指标和抗氧化能力的影响。选择健康的32头断奶仔猪,按体重相近的原则随机分为5组:对照组(基础饲粮)、LPS组(基础饲粮+LPS)、CHPS低剂量组(基础饲粮+LPS+200 mg/kg CHPS)、CHPS高剂量组(基础饲粮+LPS+800 mg/kg CHPS),每组8个重复,每个重复1头仔猪。试验期为28 d。试验期间记录每头猪日采食量,试验第1、21、28天称重,计算各阶段平均日采食量、平均日增重和料重比。试验第21天,LPS组以及CHPS低、高剂量组仔猪腹膜注射100 μg/kg BW LPS,对照组注射等量的生理盐水,注射后3 h,前腔静脉采血,分离血清,测定血清生化指标。结果表明:1)与对照组相比,应激期(22～28 d)LPS组平均日采食量和平均日增重均极显著下降(P<0.01);血清碱性磷酸酶、谷丙转氨酶活性及甘油三酯、总胆固醇、尿素氮和丙二醛含量均显著或极显著升高(P<0.05或P<0.01),一氧化氮合酶活性显著下降(P<0.05)。2)与LPS组相比,CHPS高、低剂量组平均日采食量和平均日增重均显著升高(P<0.05);CHPS低剂量组血清甘油三酯、总胆固醇和丙二醛含量均显著或极显著降低(P<0.05或P<0.01);CHPS高剂量组血清碱性磷酸酶活性极显著降低(P<0.01)。结果提示,在饲粮中添加一定剂量的CHPS能够有效缓解LPS所致免疫应激引起的断奶仔猪生长性能下降,可降低血清甘油三酯、总胆固醇、丙二醛含量及碱性磷酸酶活性,说明CHPS可以有效缓解LPS所致仔猪免疫应激。

To evaluate the effects of crude hedysari polysaccharide (CHPS) on growth performance, serum biochemical parameters and antioxidant capacity in weaner piglets challenged with lipopolysaccharide (LPS), thirty-two healthy weaner piglets were randomly allocated to control group (basal diet), LPS group (basal diet+LPS), CHPS low-dose group (basal diet+LPS+200 mg/kg CHPS), and CHPS high-dose group (basal diet+LPS+800 mg/kg CHPS), respectively. According to the records of daily feed intake by group and the body weights on days 1, 21 and 28, average daily gain (ADG), average daily feed intake (ADFI) and the ratio of feed to gain (F/G) were calculated. Piglets in the LPS group and CHPS groups were injected intraperitoneally with 100 μg/kg·BW LPS on day 21, while the piglets in the control group were injected with normal saline at the same dose. Serum samples were obtained for analysis serum biochemical parameters at 3 h post-injection. The experiment lasted for 28 days. The results showed as follows: 1) compared with the control group, LPS challenge (22 to 28 d) significantly decreased ADG and ADFI in LPS group (P<0.01), significantly increased the activities of serum alkaline phosphatase (AKP) and glutamic-pyruvic transaminase (GPT) and the contents of triglyceride (TG), total cholesterol (TC), urea nitrogen (UN) and malondialdehyde (MDA) in LPS group (P<0.05 or P<0.01), and significantly decreased nitric-oxide synthase (NOS) activity (P<0.05). 2) Compared with the LPS group, ADG and ADFI in CHPS groups were significantly increased (P<0.05), and the contents of serum TG, TC and MDA in CHPS low-dose group were significantly decreased (P<0.05 or P<0.01), and the activity of AKP in CHPS high-dose group was significantly decreased (P<0.01). These results indicate that the supplementation of CHPS in the basal diet inhibits the decrease of growth performance, decreases the contents of TG, TC and MDA and activity of AKP in the serum. CHPS can effectively relieve the immune stress of weaner piglets challenged with LPS.