动物营养学报 >

2013 , Vol. 25 >Issue 6: 1189 - 1198

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.06.010

猪营养 Swine Nutrition

饲粮中添加苏氨酸和色氨酸对接种猪繁殖与呼吸综合征弱毒苗生长猪免疫反应的影响

  • 王军 ,
  • 赵迎飞 ,
  • 方正锋 ,
  • 林燕 ,
  • 车炼强 ,
  • 杨敏 ,
  • 吴德
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  • 四川农业大学动物营养研究所,动物抗病营养教育部重点实验室,雅安 625014

收稿日期: 2012-12-03

  网络出版日期: 2013-05-15

基金资助

大学生创新性实验计划项目(01509210)

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Effects of Dietary Threonine and Tryptophan on Immune Response of Growing Pigs Inoculated Porcine Reproductive and Respiratory Syndrome Modified Live Virus Vaccine

  • WANG Jun ,
  • ZHAO Yingfei ,
  • FANG Zhengfeng ,
  • LIN Yan ,
  • CHE Lianqiang ,
  • YANG Min ,
  • WU De
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  • Key Laboratory for Animal Disease-Resistance Nutrition of the Ministry of Education of China, Institute of Animal Nutrition, Sichuan Agricultural University, Ya'an 625014, China

Received date: 2012-12-03

  Online published: 2013-05-15

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摘要

本试验旨在研究饲粮中添加苏氨酸和色氨酸对接种猪繁殖与呼吸综合征(PRRS)弱毒苗生长猪免疫反应的影响。试验选取16头平均体重为(29.11±4.41) kg健康去势生长猪,按体重相近原则随机分为4个处理,每个处理4个重复,每个重复1头猪。采用2×2两因子试验设计,设2个苏氨酸和色氨酸添加水平,按比例分别为赖氨酸:苏氨酸:色氨酸=100:64:18(NRC对照水平)和赖氨酸:苏氨酸:色氨酸=100:80:26(试验水平);2个氨基酸添加水平下按免疫处理情况分为注射磷酸盐缓冲液(PBS)和接种PRRS弱毒苗(试验开始当天和第35天肌肉注射2 mL PBS或PRRS弱毒苗)。试验第14、28、35和49天收集血样,检测血浆中干扰素-γ(IFN-γ)、白细胞介素-10(IL-10)、白细胞介素-1β(IL-1β)含量和血清中免疫球蛋白G(IgG)含量;试验第49天屠宰取样,利用实时荧光定量PCR(RT-qPCR)方法检测肺组织中Toll样受体1～10(TLR1～TLR10)mRNA相对表达量。结果表明:与饲粮添加NRC对照水平苏氨酸和色氨酸相比,注射PBS条件下,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪试验第14和28天血清IgG含量(P<0.05);接种PRRS弱毒苗条件下,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪试验第35天血清IgG含量(P<0.05)。试验第14、28和35天,接种PRRS弱毒苗生长猪血浆IFN-γ、IL-10、IL-1β含量均显著高于注射PBS生长猪(P<0.05);试验第14、28、35和49天,接种PRRS弱毒苗条件下,与饲粮添加NRC对照水平苏氨酸和色氨酸相比,饲粮添加试验水平苏氨酸和色氨酸提高了生长猪血浆IL-1β(试验第14天除外)、IFN-γ(试验第49天除外)含量,降低了血浆IL-10含量,但差异均不显著(P>0.05)。接种PRRS弱毒苗生长猪肺组织中TLR1～TLR7、TLR9、TLR10 mRNA相对表达量显著高于注射PBS生长猪(P<0.05);接种PRRS弱毒苗条件下,与饲粮添加NRC对照水平苏氨酸和色氨酸相比,饲粮添加试验水平苏氨酸和色氨酸显著提高了生长猪肺组织中TLR1～TLR3、TLR7、TLR8、TLR10 mRNA相对表达量(P<0.05),显著降低了TLR4、TLR6、TLR9 mRNA相对表达量(P<0.05)。由结果分析可知,饲粮中添加苏氨酸和色氨酸可上调TLR3、TLR7、TLR8 mRNA相对表达量,表明PRRS弱毒苗可能激活了TLR3、TLR7、TLR8信号通路,进而提高血清免疫球蛋白含量,促进炎症细胞因子产生,最终强化获得性免疫。

关键词： 苏氨酸; 色氨酸; PRRS弱毒苗; 免疫反应; 生长猪

本文引用格式

王军 , 赵迎飞 , 方正锋 , 林燕 , 车炼强 , 杨敏 , 吴德 . 饲粮中添加苏氨酸和色氨酸对接种猪繁殖与呼吸综合征弱毒苗生长猪免疫反应的影响[J]. 动物营养学报, 2013 , 25(6) : 1189 -1198 . DOI: 10.3969/j.issn.1006-267x.2013.06.010

Abstract

Sixteen healthy castrated growing pigs with an initial body weight of (29.11±4.41) kg were used to investigate the effects of dietary threonine (Thr) and tryptophan (Trp) on immune response of growing pigs inoculated with porcine reproductive and respiratory syndrome (PRRS) modified live virus vaccine. The pigs were randomly allocated to 4 treatments with 4 replicates in each treatment and 1 pig per replicate. According to a 2×2 factorial design, growing pigs received diets containing two amino acid levels [Lys:Thr:Trp = 100:64:18 (NRC level) or Lys:Thr:Trp=100:80:26 (test level)] and were injected with 2 mL of phosphate buffered saline (PBS) or PRRS modified live virus vaccine on day 0 and 35 of experiment. Blood samples were collected to determine the contents of plasma interferon-γ (IFN-γ), interleukin-10 (IL-10), interleukin-1β (IL-1β) and serum immunoglobulin G (IgG) on days 14, 28, 35 and 49 of experiment, and pigs were slaughtered to collect lung tissue on day 49 of experiment. And real-time quantitative PCR was applied to determine the relative mRNA expression levels of Toll-like receptor1 to 10 (TLR1 to TLR10) in lungs. The results showed as follows: compared with the supplementation of NRC levels of Thr and Trp, when pigs were injected with PBS, serum IgG content in pigs fed diets containing the test levels of Thr and Trp was significantly increased on days 14 and 28 of experiment (P<0.05); when pigs were injected with PRRS modified live virus vaccine, pigs fed diets containing the test levels of Thr and Trp had a higher serum IgG content on day 35 of experiment (P<0.05). Compared with PBS injection, the higher plasma IFN-γ, IL-10 and IL-1β contents were observed under immune stress condition challenged by PRRS modified live virus vaccine on days 14, 28 and 35 of experiment (P<0.05). Moreover, compared with the supplementation of NRC levels of Thr and Trp, the supplementation of test levels of Thr and Trp increased plasma IL-1β (except for day 14 of experiment) and IFN-γ (except for day 49 of experiment) contents, and decreased plasma IL-10 content on days 14, 28, 35 and 49 of experiment when pigs were injected with PRRS modified live virus vaccine (P>0.05). Compared with PBS injection, the relative mRNA expression levels of TLR1 to TLR10 of lungs were significantly increased under immune stress condition challenged by PRRS modified live virus vaccine (P<0.05); under immune stress condition challenged by PRRS modified live virus vaccine, compared with the supplementation of NRC levels of Thr and Trp, there were higher relative mRNA expression levels of TLR1 to TLR3, TLR7, TLR8 and TLR10 of lungs and lower relative mRNA expression levels of TLR4, TLR6 and TLR9 of lungs in pigs fed diets containing the test levels of Thr and Trp (P<0.05). In conclusion, the supplementation of Thr and Trp can increase the relative mRNA expression levels of TLR3, TLR7 and TLR8, indicating the PRRS modified live virus vaccine can further activate the signal pathway of TLR3, TLR7 and TLR8, which results in higher serum IgG content and inflammatory cytokines. Therefore, Thr and Trp can enhance adaptive immune response of growing pigs.

Key words： threonine; tryptophan; PRRS modified live virus vaccine; immune response; growing pigs

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