动物营养学报 >

2013 , Vol. 25 >Issue 6: 1293 - 1298

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.06.021

饲料营养 Feed Science and Technology

饲粮中添加纤维寡糖对生长猪生长性能、结肠菌群和肠黏膜通透性的影响

  • 徐露蓉 ,
  • 栾兆双 ,
  • 胡彩虹 ,
  • 石波
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  • 1. 浙江大学饲料科学研究所,动物分子营养学教育部重点实验室,杭州 310058;
    2. 中国农业科学院饲料研究所,北京 100081

收稿日期: 2012-12-24

  网络出版日期: 2013-05-15

基金资助

农业部"948"项目(2011-G7)

收起

Effects of Dietary Cello-Oligosaccharide on Growth Performance, Colonic Microflora and Intestinal Mucosal Permeability of Growing Pigs

  • XU Lourong ,
  • LUAN Zhaoshuang ,
  • HU Caihong ,
  • SHI Bo
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  • 1. Key Laboratory of Molecular Animal Nutrition of Ministry of Education, Institute of Feed Science, Zhejiang University, Hangzhou 310058, China;
    2. Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Received date: 2012-12-24

  Online published: 2013-05-15

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摘要

本试验旨在研究饲粮中添加不同水平的纤维寡糖对生长猪生长性能、结肠菌群和肠黏膜通透性的影响。将96头体重约为20 kg的"杜×长×大"生长猪按饲养试验要求分成4组,对照组饲喂基础饲粮,试验组分别饲喂在基础饲粮中添加0.1%、0.2%和0.3%纤维寡糖的试验饲粮,每组3个重复,每个重复8头猪,试验期42 d。结果表明:与对照组相比,饲粮中添加0.2%纤维寡糖使生长猪平均日增重显著提高了7.51%(P<0.05),料重比显著降低了5.93%(P<0.05),并显著增加了结肠中双歧杆菌和乳酸杆菌的数量(P<0.05),显著提高了结肠上皮细胞跨膜电阻(P<0.05),显著降低了肠上皮对荧光素异硫氰酸酯-葡聚糖的通透性(P<0.05);饲粮中添加0.3%纤维寡糖对上述各指标有显著影响(P<0.05),但与添加0.2%纤维寡糖作用效果无显著差异(P>0.05);饲粮中添加0.1%纤维寡糖对上述各指标无显著影响(P>0.05)。结果提示,饲粮中添加纤维寡糖可以提高生长猪的生长性能,刺激结肠肠道有益菌的增殖并抑制有害菌的增殖,改善结肠黏膜屏障。本试验条件下,纤维寡糖在生长猪饲粮中的适宜添加水平为0.2%。

关键词： 纤维寡糖; 生长性能; 肠黏膜屏障; 生长猪

本文引用格式

徐露蓉 , 栾兆双 , 胡彩虹 , 石波 . 饲粮中添加纤维寡糖对生长猪生长性能、结肠菌群和肠黏膜通透性的影响[J]. 动物营养学报, 2013 , 25(6) : 1293 -1298 . DOI: 10.3969/j.issn.1006-267x.2013.06.021

Abstract

This experiment was conducted to investigate the effects of dietary cello-oligosaccharide on growth performance, colonic microflora and intestinal mucosal permeability of growing pigs. A total of 96 growing pigs (Duroc×Landrace×Yorkshire) with an average body weight of 20 kg were randomly allocated into 4 groups with 3 replicates per group and 8 pigs per replicate. Pigs in the control group were fed a basal diet, and pigs in the experimental groups were fed the basal diet supplemented with 0.1%, 0.2% and 0.3% cello-oligosaccharide, respectively. The experiment lasted for 42 days. The results showed as follows: compared with the control group, pigs fed diets with 0.2% cello-oligosaccharide had the best performance, the average daily gain was significantly increased by 7.51% (P<0.05), and feed/gain was significantly decreased by 5.93% (P<0.05); the supplementation of 0.2% cello-oligosaccharide significantly increased the number of Bifidobacterium and Lactobacillus (P<0.05), and significantly reduced the number of Escherichia coli and Clostridium in colon (P<0.05); through the Ussing chamber technology, pigs fed diets with 0.2% cello-oligosaccharide had higher transepithelial electrical resistance (TER) and lower mucosal-to-serosal flux of 4 ku-fluorescein isothiocyanate-dextran (FD4) permeability in colonic epithelium (P<0.05). The supplementation of 0.3% cello-oligosaccharide had significant effects on above indexes (P<0.05), but there was no significant difference between 0.3% and 0.2% cello-oligosaccharide supplementation (P>0.05). The supplementation of 0.1% cello-oligosaccharide did not affect above indexes (P>0.05). In conclusion, the supplementation of cello-oligosaccharide can significantly improve the growth performance, colonic microflora and colonic mucosal barrier function of growing pigs. The optimal supplemental level of cello-oligosaccharide for growing pigs is 0.2%.

Key words： cello-oligosaccharide; growth performance; intestinal mucosal barrier; growing pigs

参考文献

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