为了研究非淀粉多糖酶对玉米加工副产品氨基酸及养分真代谢率的影响,试验选用56只平均体重为(2.5±0.2) kg的健康成年黄羽肉公鸡,采用完全随机试验设计,将其分为7组,每组8个重复,每个重复1只鸡,采用绝食强饲法进行3期代谢试验,以单一原料+复合非淀粉多糖酶为强饲料,测定玉米及其加工副产品中添加复合非淀粉多糖酶前后的氨基酸真代谢率。结果表明:加酶前,氨基酸平均真代谢率以玉米最高,喷浆玉米皮(山东济宁)最低;加酶后,各原料的氨基酸真代谢率在70.77%～99.29%,氨基酸真代谢率提高了0.96%～3.52%,且酶制剂对玉米及玉米蛋白粉[粗蛋白质(CP) 50%、CP 55%]的多种氨基酸真代谢率有显著的提升作用(P<0.05),而真可利用氨基酸的有效营养改进值(ENIV)范围为0.03～1.87 g/kg,其中玉米蛋白粉(CP 55%)的平均ENIV最高,玉米麸质饲料最低。酶制剂的添加有提高多种玉米加工副产品干物质、CP及粗脂肪真代谢率的趋势(P>0.05),对某些原料干物质及CP真代谢率有显著提高效果(P<0.05),对玉米及其加工副产品粗纤维真代谢率也有显著提高作用(P<0.05)。由此可知,添加复合非淀粉多糖酶总体上能提高玉米及其加工副产品的氨基酸及养分真代谢率,改善玉米加工副产品的营养效价。

To study the effects of non-starch polysaccharide-degrading enzymes on true amino acid and nutrient metabolic rates of corn by-products, a complete randomized design and three metabolic test periods had been taken, and 56 healthy adult yellow-feathered roosters with an average body weight of (2.5?0.2) kg were randomly divided into 7 groups with 8 replicates in each group and 1 rooster in each replicate. The roosters were force-fed with a single raw material and the material with non-starch polysaccharide-degrading enzymes to determine the true amino acid metabolic rates of corn and its by-products before and after the enzyme addition. Results showed that before the enzyme addition, the average true amino acid metabolic rate of corn was the highest, and the lowest one was germ (no spray, from Jining). After the enzyme was added, the true amino acid metabolic rates of corn by-products were between 70.77% to 99.29%, and the enzyme increased the true amino acid metabolic rates of corn by-products by 0.96% to 3.52%. The true metabolic rates of many amino acids in corn and corn gluten meal [crude protein (CP) 50% and CP 55%] were significantly increased by the supplementation of the enzyme (P<0.05). And the effective nutrient improvement values (ENIV) of true available amino acid contents of corn by-products were in the range of 0.03 to 1.87 g/kg, the highest was corn gluten meal (CP 55%), and the lowest one was corn fiber feed. The supplementation of the enzyme can improve the metabolic rates of true CP, dry matter (DM) and ether extract (EE) of varieties of corn by-products (P>0.05), and for some by-products it could significantly increase the true metabolic rates of DM and CP (P<0.05). The enzyme could also significantly enhance the true crude fiber (CF) metabolic rate of all corn by-products (P<0.05). Generally speaking, complex non-starch polysaccharide-degrading enzymes can improve the true amino acid and nutrient metabolic rates of corn and its by-products.