综述 Review

miRNA在脂代谢中的研究进展

  • 王来娣 ,
  • 郑云 ,
  • 蒋拾贝 ,
  • 王星果 ,
  • 张军 ,
  • 龚道清
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  • 扬州大学动物科学与技术学院 扬州 225009

收稿日期: 2013-01-06

  网络出版日期: 2013-06-17

基金资助

江苏省科技支撑计划项目(BE2011328)

Research Advances of miRNA in Lipid Metabolism

  • WANG Laidi ,
  • ZHENG Yun ,
  • JIANG Shibei ,
  • WANG Xingguo ,
  • ZHANG Jun ,
  • GONG Daoqing
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2013-01-06

  Online published: 2013-06-17

摘要

脂肪是人和动物用以贮存能量的主要形式,脂类代谢在机体生命活动中发挥着重要作用,而脂类代谢调控对于畜牧生产以及人类疾病治疗都有重要意义。miRNA(microRNA,译作微RNA或小分子RNA)是近年来在真核生物体内发现的一类长度约22个核苷酸的内源性非编码单链RNA,主要通过与靶基因mRNA靶标区域的互补配对,发挥降解靶mRNA或抑制mRNA翻译的作用。它能参与多种生物学过程包括细胞凋亡、分化和癌变等,近几年其关于脂代谢的重要调节作用也相继被报导。本文主要对调节脂代谢的一些关键miRNA的研究进行综述。

关键词: miRNA; 脂代谢; 靶基因

本文引用格式

王来娣 , 郑云 , 蒋拾贝 , 王星果 , 张军 , 龚道清 . miRNA在脂代谢中的研究进展[J]. 动物营养学报, 2013 , 25(7) : 1446 -1452 . DOI: 10.3969/j.issn.1006-267x.2013.07.007

Abstract

Fat is the main form for energy storage in human beings and animals. Lipid metabolism plays an important role in a variety of life activities, and the regulation has important implications for livestock production and treatments of human diseases. miRNAs (microRNAs) are found in eukaryotes, and they are a class of non-coding single-stranded RNA molecules with the length of about 22 nt. miRNAs mainly act the functions of destabilization and translational repression of mRNA by binding to complementary target sites in target mRNAs. miRNAs take parts in regulating multiple physiological processes including apoptosis, cell differentiation, and canceration, ect. The importance of these miRNAs in regulating lipid metabolism has been reported recently. This review summarized the researches on some key miRNAs in regulating lipid metabolism.

参考文献

[1] 樊红平,侯水生.家禽体内脂肪沉积调控的研究进展[J].动物营养学报,2004,16(4):1-6.

[2] 吴自光,刘相玉.影响乳脂的因素及提高乳脂率的方法[J].山东农业科学,2004,6:54-55.

[3] 季海峰,张沅.猪脂肪代谢的研究进展[J].中国畜牧杂志,1993,29(3):59-61.

[4] ALBERTI K G,ZIMMET P,SHAW J.Metabolic syndrome:a new world-wide definition.A consensus statement from the international diabetes federation[J].Diabetic Medicine,2006,23(5):469-480.

[5] TERAN-GARCIA M,BOUCHARD C.Genetics of the metabolic syndrome[J].Applied Physiology Nutrition and Metabolism,2007,32:89-114.

[6] BARTEL D P.MicroRNAs:genomics,biogenesis,mechanism and function[J].Cell,2004,116(2):281-297.

[7] BRENNECKE J,STARK A,RUSSELL R B,et al.Principles of microRNA-target recognition[J].PLoS Biology,2005,3(3):e85.

[8] KREK A,GRUN D,POY M N,et al.Combinatorial microRNA target predictions[J].Nature Genetics,2005,37(5):495-500.

[9] DOENCH J G,SHARP P A.Specificity of microRNA target selection in translational repression[J].Genes and Development,2004,18(5):504-511.

[10] 李龙江,余瑜.脂代谢的信号转导途径[J].儿科药学杂志,2005,11(1):7-9.

[11] BERGER J,LEIBOWITZ M D,DOEBBER T W,et al.Novel peroxisome proliferator activated receptors (PPAR)γ and PPARδ ligands produce distinct biological effects[J].Journal of Biological Chemistry,1999,274:6178-6725.

[12] LEE C H,OLSON P,EVANS R M.Mini-review:lipid metabolism,metabolic diseases and peroxisome proliferator activated receptors[J].Endocrinology,2003,144(6):2201-2207.

[13] BOITIER E,GAUTIER J C,ROBERTS R.Advances in understanding the regulation of apoptosis and mitosis by peroxisome-proliferator activated receptors in pre-clinical models:relevance for human health and disease[J].Comparative Hepatology,2003,2(1):3-17.

[14] CHAWLA A,LEE C H,BARAK Y,et al.PPAR delta is a very low density lipoprotein sensor in macrophages[J].Proceedings of the National Academy of Sciences of the United States of America,2003,100(3):1268-1273.

[15] HORTON J D,GOLDSTEIN J L,BROWN M S.SREBPs:activators of the complete program of cholesterol and fatty acid synthesis in the liver[J].Journal of Clinical Investigation,2002,109:1125-1131.

[16] BROWN M S,GOLDSTEIN J L.The SREBP pathway:regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor[J].Cell,1997,89:331-340.

[17] OSBORNE T F.Sterol regulatory element-binding proteins (SREBPs):key regulators of nutritional homeostasis and insulin action[J].Journal of Biological Chemistry,2000,275:32379-32382.

[18] PEET D J,JANOWSKI B A,MANGELSDORF D J.The LXRs:a new class of oxysterol receptors[J].Current Opinion in Genetics and Development,1998,8(5):571-575.

[19] TONTONOZ P,MANGELSDORF D J.Liver X receptor signaling pathways in cardiovascular disease[J].Molecular Endocrinology,2003,17:985-993.

[20] BRADLEY M N,HONG C,CHEN M,et al.Ligand activation of LXR beta reverses atherosclerosis and cellular cholesterol overload in mice lacking LXR alpha and apoE[J].Journal of Clinical Investigation,2007,117:2337-2346.

[21] CLAUDEL T,LEIBOWITZ M D,FIEVET C,et al.Reduction of atherosclerosis in apolipoprotein E knockout mice by activation of the retinoid X receptor[J].Proceedings of the National Academy of Sciences of the United States of America,2001,98(5):2610-2615.

[22] JOSEPH S B,MCKILLIGIN E,PEI L,et al.Synthetic LXR ligand inhibits the development of atherosclerosis in mice[J].Proceedings of the National Academy of Sciences of the United States of America,2002,99:7604-7609.

[23] REPA J J,LIANG G,OU J,et al.Regulation of mouse sterol regulatory element-binding protein-1c gene (SREBP-1c) by oxysterol receptors LXR-alpha and LXR-beta[J].Genes and Development,2000,14:2819-2830.

[24] PEET D J,TURLEY S D,MA W,et al.Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXR-alpha[J].Cell,1998,93(5):693-704.

[25] CHEN G,LIANG G,OU J,et al.Central role for liver X receptor in insulin-mediated activation of SREBP-1C transcription and stimulation of fatty acid synthesis in liver[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101:11245-11250.

[26] OTA A,TAGAWA H,KARNAN S,et al.Identification and characterization of a novel gene,C13orf25,as a target for 13q31-q32 amplification in malignant lymphoma[J].Cancer Research,2004,64(9):3087-3095.

[27] LEE R C,FEINBAUM R L,AMBROS V.The C.elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14[J].Cell,1993,75:843-854.

[28] RODRIGUEZ A,GRIFFITHS J S,ASHURST J L,et al.Identification of mammalian microRNA host genes and transcription units[J].Genome Research,2004,14:1902-1910.

[29] LEE Y,AHN C,HAN J,et al.The nuclear RNase Ⅲ Drosha initiates microRNA processing[J].Nature,2003,425:415-419.

[30] LEE Y,JEON K,LEE J T,et al.MicroRNA maturation:stepwise processing and subcellular localization[J].The EMBO Journal,2002,21:4663-4670.

[31] BERNSTEIN E,CAUDY A A,HAMMOND S M,et al.Role for abidentate ribonuclease in the initiation step of RNA interference[J].Nature,2001,409:363-366.

[32] YEOM K H,LEE Y,HAN J,et al.Characterization of DGCR8/Pasha,the essential cofactor for Drosha in primary miRNA processing[J].Nucleic Acids Research,2006,34:4622-4629.

[33] LIU J,CARMELL M A,RIVAS F V,et al.Argonaute 2 is the catalytic engine of mammalian RNAi[J].Science,2004,305:1437-1441.

[34] HAMMOND S M,BERNSTEIN E,BEACH D,et al.An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells[J].Nature,2000,404:293-296.

[35] LIU J,VALENCIA-SANCHEZ M A,HANNON G J,et al.MicroRNA-dependent localization of targeted mRNAs to mammalian P-bodies[J].Nature Cell Biology,2005,7:719-723.

[36] LAI E C.MicroRNAs are complementary to 3'UTR sequence motifs that mediate negative post-transcriptional regulation[J].Nature Genetics,2002,30:363-364.

[37] SAXENA S,JONSSON Z O,DUTTA A.Small RNAs with imperfect match to end ogenous mRNA repress translation.Implications for off-target activity of small inhibitory RNA in mammalian cells[J].Journal of Biological Chemistry,2003,278:44312-44319.

[38] NAJAFI-SHOUSHTARI S H,FJORALBA K,LI Y X,et al.MicroRNA-33 and the SREBP host genes cooperate to control cholesterol homeostasis[J].Science,2010,328:1566-1569.

[39] RAYNER K J,SHEEDY F J,ESAU C C.Antagonism of miR-33 in mice promotes reverse cholesterol transport and regression of atherosclerosis[J].Journal of Clinical Investigation,2011,121(7):2921-2931.

[40] RAYNER K J,SUAREZ Y,DAVALOS A,et al.MiR-33 contributes to the regulation of cholesterol homeostasis[J].Science,2010,328:1570-1573.

[41] RAYNER K J,ESAU C C,HUSSAIN F N,et al.Inhibition of miR-33a/b in non-human primates raises plasma HDL and reduces VLDL triglycerides[J].Nature,2012,478:404-407.

[42] TSAI W C,HSU S D,HSU C S,et al.MicroRNA-122 plays a critical role in liver homeostasis and hepatocarcinogenesis[J].Journal of Clinical Investigation,2012,122(8):2773-2897.

[43] CHANG J,NICOLAS E,MARKS D,et al.miR-122,a mammalian liver-specific microRNA,is processed from hcr mRNA and may down-regulate the high affinity cationic amino acid transporter CAT-1[J].RNA Biology,2004,1:106-113.

[44] ESAU C,DAVIS S,MURRAY S F,et al.miR-122 regulation of lipid metabolism revealed by in vivo antisense targeting[J].Cell Metabolism,2006,3(2):87-98.

[45] KOJIMA S,GATFIELD D,ESAU C C,et al.MicroRNA-122 modulates the rhythmic expression profile of the circadian deadenylase nocturnin in mouse liver[J].PLoS One,2010,5(6):e11264.

[46] ELMÉN J,LINDOW M,SILAHTAROGLU A,et al.Antagonism of microRNA-122 in mice by systemically administered LNA-antimiR leads to upregulation of a large set of predicted target mRNAs in the liver[J].Nucleic Acids Research,2008,36:1153-1162.

[47] CHEN W J,YIN K,ZHAO G J,et al.The magic and mystery of microRNA-27 in atherosclerosis[J].Atherosclerosis.2012,222(2):314-323.

[48] KIM S Y,KIM A Y,LEE H W,et al.miR-27a is a negative regulator of adipocyte differentiation via suppressing PPAR-γ expression[J].Biochemical and Biophysical Research Communications,2010,392:323-328.

[49] KARBIENER M,FISCHER C,NOWITSCH S,et al.MicroRNA miR-27b impairs human adipocyte differentiation and targets PPAR gamma[J].Biochemical and Biophysical Research Communications,2009,390:247-251.

[50] KIDA K,NAKAJIMA M,MOHRI T,et al.PPAR alpha is regulated by miR-21 and miR-27b in human liver[J].Pharmaceutical Research,2011,28:2467-2476.

[51] WANG T,LI M Z,GUAN J Q,et al.MicroRNAs miR-27a and miR-143 regulate porcine adipocyte lipid metabolism[J].International Journal of Molecular Sciences,2011,12:7950-7959.

[52] NAKANISHI N,NAKAGAWA Y,TOKUSHIGE N,et al.The up-regulation of microRNA-335 is associated with lipid metabolism in liver and white adipose tissue of genetically obese mice[J].Biochemical and Biophysical Research Communications,2009,385:492-496.

[53] GERIN I,BOMMER G T,MCCOIN C S,et al.Roles for miRNA-378/378* in adipocyte gene expression and lipogenesis[J].American Journal of Physiology-endocrinology and Metabolism,2010,299:198-206.

[54] VINCIGUERRA M,SGROI A,VEYRAT-DUREBEX C,et al.Unsaturated fatty acids inhibit the expression of tumor suppressor phosphatase and tensin homolog (PTEN) via microRNA-21 up-regulation in hepatocytes[J].Hepatology,2009,49(4):1176-1184.

[55] ESAU C,KANG X L,PERALTA E,et al.MicroRNA-143 regulates adipocyte differentiation[J].Journal of Biological Chemistry,2004,279(50):52361-52365.
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