饲料营养 Feed Science and Technology

饲料中添加抗菌肽对锦鲤生长、非特异性免疫力和抗病力的影响

  • 林鑫 ,
  • 毛述宏 ,
  • 杨阳 ,
  • 林仕梅 ,
  • 罗琳
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  • 1. 西南大学淡水鱼类资源与生殖发育教育部重点实验室,重庆 400716;
    2. 西南大学动物科技学院, 重庆 400716;
    3. 北京市水产科学研究所,北京 100068

收稿日期: 2013-05-06

  网络出版日期: 2013-07-15

基金资助

北京市科学技术委员会项目(项目编号:D09060500430000)

Effects of Antimicrobial Peptides Supplementation on Growth, Non-Specific Immunity and Disease Resistance of Koi (Cyprinus carpio koi)

  • LIN Xin ,
  • MAO Shuhong ,
  • YANG Yang ,
  • LIN Shimei ,
  • LUO Lin
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  • 1. Key Laboratory of Freshwater Fish Reproduction and Development, Ministry of Education, Chongqing 400716, China;
    2. College of Animal Science and Technology, Southwest University, Chongqing 400716, China;
    3. Beijing Fisheries Research Institute, Beijing 100086, China

Received date: 2013-05-06

  Online published: 2013-07-15

摘要

本试验旨在研究饲料中添加抗菌肽对锦鲤生长、非特异性免疫力和抗病力的影响.在基础饲粮中分别添加0(对照)、75、150和225 mg/kg的天蚕素抗菌肽,配制成4种等氮(粗蛋白质32%)等能(总能14.8 MJ/kg)的试验饲料,并分别饲喂平均体重为(10.30±0.01) g的锦鲤8周.每组设4个重复,每个重复35尾鱼.结果表明:150 mg/kg组锦鲤的末重和特定生长率(SGR)均显著高于对照组和225 mg/kg组(P<0.05),而与75 mg/kg组差异不显著(P>0.05).锦鲤的饲料系数(FCR)、蛋白质效率(PER)和摄食量(FI)组间差异不显著(P>0.05).225 mg/kg组锦鲤的血清过氧化氢酶(CAT)、总超氧化物歧化酶(T-SOD)和碱性磷酸酶(AKP)活性显著高于对照组和75 mg/kg组(P<0.05),与150 mg/kg组差异不显著(P>0.05).各组锦鲤的血清溶菌酶(LZM)活性无显著差异(P>0.05).用维氏气单胞菌攻毒后,各抗菌肽添加组锦鲤的累积死亡率显著低于对照组(P<0.05).由此可见,饲料中添加抗菌肽可以增强锦鲤的非特异性免疫力和抗病力,进而促进其生长;锦鲤饲料中天蚕素抗菌肽的适宜添加量为150 mg/kg.

本文引用格式

林鑫 , 毛述宏 , 杨阳 , 林仕梅 , 罗琳 . 饲料中添加抗菌肽对锦鲤生长、非特异性免疫力和抗病力的影响[J]. 动物营养学报, 2013 , 25(8) : 1860 -1865 . DOI: 10.3969/j.issn.1006-267x.2013.08.024

Abstract

This experiment was conducted to evaluate the effects of antimicrobial peptides (ABPs) supplementation on growth, non-specific immunity and disease resistance of koi (Cyprinus carpio koi). Four isonitrogenous (crude protein level was 32%) and isoenergetic (gross energy was 14.8 MJ/kg) experimental diets were formulated by supplementing with 0 (control), 75, 150 and 225 mg/kg ABPs—cecropin, respectively. Each diet was randomly fed one group of fish with an initial average weight of (10.30±0.01) g for 8 weeks. Each group had 4 replicates with 35 fish per replicate. The results showed as follows: the final body weight, specific growth rate (SGR) of koi in 150 mg/kg group were significantly higher than those in control group and 225 mg/kg group (P<0.05), whereas there were no differences between 75 and 150 mg/kg groups (P>0.05). No significant differences were found in feed conversion ratio (FCR), protein efficiency ratio (PER) and feed intake (FI) of koi among all groups (P>0.05). Serum alkaline phosphatase (AKP), catalase (CAT) and superoxide dismutase (T-SOD) activities in 225 mg/kg group were significantly higher than those in control group and 75 mg/kg group (P<0.05), whereas there were no differences between 150 and 225 mg/kg groups (P>0.05). There was no difference in serum lysozyme (LZM) activity of koi among all groups (P>0.05). Moreover, the ABPs supplementation groups also exhibited a significant decrease in cumulative mortality compared with control group when challenged with Aeromonas veronii (P<0.05). It is concluded that ABPs supplementation can enhance the non-specific immunity and disease resistance, and then promote the growth of koi. And the optimum supplemental level of cecropin in the diet of koi is 150 mg/kg.

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