不同类型饲粮饲喂下奶牛瘤胃乳头的光镜和透射电镜观察

翁秀秀; 张养东; 李发弟; 周凌云; 卜登攀

doi:10.3969/j.issn.1006-267x.2013.09.011

动物营养学报 >

2013 , Vol. 25 >Issue 9: 1998 - 2004

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.011

反刍动物营养 Ruminant Nutrition

不同类型饲粮饲喂下奶牛瘤胃乳头的光镜和透射电镜观察

翁秀秀 ,
张养东 ,
李发弟 ,
周凌云 ,
卜登攀

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1. 中国农业科学院北京畜牧兽医研究所, 动物营养学国家重点实验室, 北京 100193;
2. 甘肃农业大学动物科学技术学院, 兰州 730070

收稿日期: 2013-03-02

网络出版日期: 2013-08-18

基金资助

国家"973"计划项目(2011CB100805);"十二五"科技支撑项目(2012BAD12B02);重点实验室开放课题(2004DA125184G1103)

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Light and Transmission Electron Microscopical Observation of Ruminal Papillae of Dairy Cows Receiving Different Types of Diets

WENG Xiuxiu ,
ZHANG Yangdong ,
LI Fadi ,
ZHOU Lingyun ,
BU Dengpan

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1. State Key Laboratory of Ruminant Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China

Received date: 2013-03-02

Online published: 2013-08-18

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摘要

为了探讨不同类型饲粮对奶牛瘤胃乳头组织形态学的影响,试验选择10头初产、带有永久性瘤胃瘘管的泌乳中期[(127±13)d]荷斯坦奶牛,随机分成2组,每组5头,分别饲喂2种饲粮,一种以玉米秸秆为唯一粗饲料来源并添加高水平精料,该组称为单一秸秆组(CS组);另一种以羊草、苜蓿干草、全株玉米青贮为粗饲料来源并添加低水平精料,该组称为混合粗料组(MF组),试验期63 d。试验结束经瘤胃瘘管活体采集瘤胃乳头,制备切片,应用光学显微镜和透射电镜对瘤胃乳头的组织结构及超微结构进行观察。结果表明:CS组奶牛的瘤胃乳头宽度极显著高于MF组(P<0.01)。MF组奶牛的瘤胃乳头表面上皮的颗粒层、棘层和基底层的厚度均高于CS组,角质层厚度低于CS组,但组间差异均不显著(P>0.05)。CS组奶牛瘤胃乳头颗粒层的细胞连接退化,细胞之间存在较大的空隙;角质层有少量的蜕皮现象,角质层下的细胞层细胞呈现加速的迁移状态。由此可见,给泌乳奶牛饲喂玉米秸秆并添加高水平精料的饲粮影响了瘤胃乳头的形态学结构。

关键词： 饲粮类型; 瘤胃乳头; 组织形态学

本文引用格式

翁秀秀 , 张养东 , 李发弟 , 周凌云 , 卜登攀 . 不同类型饲粮饲喂下奶牛瘤胃乳头的光镜和透射电镜观察[J]. 动物营养学报, 2013 , 25(9) : 1998 -2004 . DOI: 10.3969/j.issn.1006-267x.2013.09.011

Abstract

The objective of this study was to determine the effects of different types of diets on ruminal papillae histomorphology of dairy cows. Ten primiparous and rumen-cannulated Holstein dairy cows averaging (127?13) d in milk were randomly assigned to 2 groups, and each group had 5 dairy cows. The dairy cows were fed two types of diets, one of them was formulated with corn straw as sole forage and high level concentrate (CS group) and the other was formulated with chinese wildrye+alfalfa hay+corn silage as mixed forage and low level concentrate (MF group). The experiment lasted for 63 days. At the end of this experiment, ruminal papillae were biopsied from the ventral sac and observed using light and transmission electron microscope. The results showed as follows: the width of papillae in CS group was significantly higher than that in MF group (P<0.01). The thickness of stratum granulosum, stratum spinosum, and stratum basale in MF group were higher than those in CS group, the thickness of stratum corneum was lower than that in CS group, but no significant differences were found between the two groups (P>0.05). Biopsied papillae exhibited a decline in cellular junctions and large spaces between cells of stratum granulosum, moderate sloughing of the stratum corneum, and an accelerated migratory state in all strata below the stratum corneum in CS group. These results suggest that feeding the diet formulated with corn straw and high level concentrate affects ruminal papillae morphological structure of dairy cows.

Key words： dietary types; ruminal papillae; histomorphology

参考文献

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