动物营养学报 >

2013 , Vol. 25 >Issue 9: 2012 - 2020

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.09.013

反刍动物营养 Ruminant Nutrition

不同NFC/NDF饲粮和硫胺素对奶牛瘤胃代谢的影响

  • 蔡晶晶 ,
  • 王洪荣 ,
  • 付聪 ,
  • 李志腾 ,
  • 朱婧靓
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  • 扬州大学动物科学与技术学院, 扬州 225009

收稿日期: 2012-03-12

  网络出版日期: 2013-08-18

基金资助

国家自然科学基金项目(31072051)

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Effects of Different Dietary NFC/NDF and Thiamine on Rumen Metabolism in Dairy Cows

  • CAI Jingjing ,
  • WANG Hongrong ,
  • FU Cong ,
  • LI Zhiteng ,
  • ZHU Jingjing
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  • College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China

Received date: 2012-03-12

  Online published: 2013-08-18

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摘要

本试验旨在研究不同非纤维性碳水化合物和中性洗涤纤维比(NFC/NDF)饲粮和硫胺素对荷斯坦奶牛瘤胃代谢的影响。选用3头健康状况良好且安装永久性瘤胃瘘管的荷斯坦奶牛,采用3×3完全拉丁方试验设计。试验分3期进行,每期23 d,每期中预试期20 d,正试期3 d;3个试验组分别饲喂NFC/NDF为1.41(精粗比5:5)、NFC/NDF为2.01(精粗比7:3)、NFC/NDF为2.01(精粗比7:3)+180 mg/kg硫胺素的试验饲粮。结果表明:提高饲粮NFC/NDF,瘤胃液pH显著降低(P<0.05),氨态氮(NH3-N)浓度显著或极显著上升(P<0.05或P<0.01),乳酸浓度显著或极显著升高(P<0.05或P<0.01),乙酸比例(P<0.05)、乙酸/丙酸(P<0.05或P<0.01)显著或极显著降低,丙酸、丁酸比例显著或极显著上升(P<0.05或P<0.01)。高NFC/NDF饲粮添加硫胺素后,瘤胃液pH有升高趋势,差异不显著(P>0.05),NH3-N浓度显著降低(P<0.05),乳酸浓度显著或极显著降低(P<0.05或P<0.01),乙酸比例和乙酸/丙酸显著或极显著降低(P<0.05或P<0.01),丙酸比例显著或极显著升高(P<0.05或P<0.01)。由此可见,饲粮NFC/NDF的增加能够诱导瘘管奶牛产生亚急性瘤胃酸中毒,添加180 mg/kg硫胺素能够缓解这一现象。

关键词： NFC/NDF; 硫胺素; 亚急性瘤胃酸中毒; 瘤胃发酵

本文引用格式

蔡晶晶 , 王洪荣 , 付聪 , 李志腾 , 朱婧靓 . 不同NFC/NDF饲粮和硫胺素对奶牛瘤胃代谢的影响[J]. 动物营养学报, 2013 , 25(9) : 2012 -2020 . DOI: 10.3969/j.issn.1006-267x.2013.09.013

Abstract

This experiment was conducted to study the effects of different dietary non-fiber carbohydrates to neutral detergent fiber ratios (NFC/NDF) and thiamine on rumen metabolism in Holstein dairy cows. Three healthy Holstein dairy cows equipped with rumen fistulous were used in a 3?3 Latin square design experiment. The experiment was carried out for 3 periods with 23 days (20-day pre-trial period and 3-day trial period) per period. The cows were fed three different diets, which were NFC/NDF=1.41 (concentrate to forage 5:5), NFC/NDF=2.01 (concentrate to forage 7:3) and NFC/NDF=2.01 (concentrate to forage 7:3)+180 mg/kg thiamine, respectively. The results showed as follows: with the increase of dietary NFC/NDF, rumen fluid pH was significantly decreased (P<0.05), the NH3-N concentration of rumen fluid was significantly increased (P<0.05 or P<0.01), and the lactic acid concentration of rumen fluid was also significantly increased (P<0.05 or P<0.01), while the acetic acid proportion (P<0.05) and acetic acid/propionic acid (P<0.05 or P<0.01) of rumen fluid were significantly decreased, and the proportions of propionic acid proportion and butyric acid of rumen fluid were significantly increased (P<0.05 or P<0.01). The dietary supplementation of thiamine tended to increase rumen fluid pH (P>0.05), while rumen fluid NH3-N concentration was significantly decreased (P<0.05), lactic acid concentration was decreased significantly (P<0.05 or P<0.01), and acetic acid proportion and acetic acid/propionic acid of rumen fluid were significantly decreased (P<0.05), while propionic acid proportion was significantly increased (P<0.05 or P<0.01). These results indicate that the increase of dietary NFC/NDF can induce subactue ruminal acidosis, and the alterative dietary supplementation of thiamine at 180 mg/kg can release the rumen acidosis.

Key words： NFC/NDF; thiamin; subactue ruminal acidosis; rumen fermentation

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