分子营养 Molecular Nutrition

基于16SrRNA基因序列分析梅花鹿瘤胃细菌多样性

  • 李志鹏 ,
  • 刘晗璐 ,
  • 崔学哲 ,
  • 荆祎 ,
  • 鲍坤 ,
  • 徐超 ,
  • 杨福合 ,
  • 李光玉
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  • 中国农业科学院特产研究所, 吉林省特种经济动物分子重点实验室, 长春 130112

收稿日期: 2013-03-07

  网络出版日期: 2013-08-18

基金资助

国家科技支撑计划(2011BAI03B02)

Bacterial Diversity in Rumen of Sika Deer (Cervus nippon) Based on 16S rRNA Sequences Analysis

  • LI Zhipeng ,
  • LIU Hanlu ,
  • CUI Xuezhe ,
  • JING Yi ,
  • BAO Kun ,
  • XU Chao ,
  • YANG Fuhe ,
  • LI Guangyu
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  • Jilin Provincial Key Labororary for Molecular Biology of Special Economic Animals, Insitute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received date: 2013-03-07

  Online published: 2013-08-18

摘要

本试验旨在对以柞树叶为主要粗饲料来源的梅花鹿瘤胃细菌多样性进行分析。提取瘤胃微生物基因组DNA,扩增细菌16S rRNA基因,构建16S rRNA基因克隆文库,分析梅花鹿瘤胃细菌区系组成。结果表明:1)试验共得到107个非嵌合体16S rRNA基因序列。按照97%序列相似性,划分为22个分类操作单元(OTUs)。其中91个序列(10个OTUs,85%总克隆序列)与已培养菌序列相似性≥97%,13个序列(10个OTUs,12.2%总克隆序列)与已培养菌序列相似性为90%~97%,其余序列相似性<90%。87.9%序列与普雷沃氏菌属(Prevotella spp.)相似。2)系统发育分析表明,梅花鹿瘤胃细菌由厚壁菌门和拟杆菌门组成。由结果可知,以富含单宁的柞树叶为主要粗饲料来源的梅花鹿的瘤胃中,普雷沃氏菌属是优势细菌,而牛、羊瘤胃中常见的纤维素降解菌未检测到,这可能与饲料中单宁含量高有关。

本文引用格式

李志鹏 , 刘晗璐 , 崔学哲 , 荆祎 , 鲍坤 , 徐超 , 杨福合 , 李光玉 . 基于16SrRNA基因序列分析梅花鹿瘤胃细菌多样性[J]. 动物营养学报, 2013 , 25(9) : 2044 -2050 . DOI: 10.3969/j.issn.1006-267x.2013.09.017

Abstract

Bacteiral diversity in the rumen of sika deer feeding tannin-rich plants (oak leaves) was investigated. The extrated genomic DNA was used to amplify bacteria 16S rRNA gene. Bacterial communities in the rumen of sika deer were determined based on the 16S rRNA gene library. The results showed as follows: 1) a total of 107 non-chimeric 16S rRNA gene sequences were obtained. These sequences were assinged to 22 operational taxonomic units (OTUs) based on 97% sequence similarity. Ninety-one sequences (10 OTUs, 85% of total clones) showed ≥97% sequence similarity to known species, thirteen sequences (10 OTUs, 12.2% of total clones) had sequence similarity to known species in the range of 90% to 97%, and the remaining sequences displayed similarity less than 90%. Eighty-seven point nine percent sequences were similar to Prevotella spp.. 2) Phylogenetic analysis indicated that bacteria in the rumen of sika deer belonged to phyla Bacteriodetes and Firmicutes. It is concluded that Prevotella spp. is the dominant bacteria in rumen of sika deer feeding oak leaves containing high content of tannins. While, the lack of fibrolytic bactria in the rumen of sika deer may be the result of hight content of tannins in diets.

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