本试验旨在研究丁氨丙磷溶液对肉仔鸡生长性能和免疫功能的影响。选取健康1日龄黄羽肉鸡180羽,随机分为3组(Ⅰ、Ⅱ、Ⅲ组),每组6个重复,每个重复10羽。Ⅰ组肌肉注射生理盐水,Ⅱ组肌肉注射国产丁氨丙磷溶液,Ⅲ组肌肉注射进口丁氨丙磷溶液,分3次注射,第1次于1日龄注射0.2 mL/只,第2次于21日龄注射0.5 mL/只,第3次于49日龄注射1.0 mL/只。试验期56 d。结果表明:1)56日龄时,平均日采食量Ⅱ组和Ⅲ组均比Ⅰ组显著性增加(P<0.05),平均日增重Ⅱ组比Ⅰ组显著性提高(P<0.05),Ⅲ组比Ⅰ组极显著性提高(P<0.01),但是3组间料重比差异不显著(P>0.05)。此外,丁氨丙磷溶液极显著地降低了发病率(P<0.01)和显著降低了死亡率(P<0.05)。2)28日龄和56日龄时,Ⅱ组和Ⅲ组肉仔鸡血清中新城疫(克隆30)弱毒苗的抗体水平均极显著高于Ⅰ组(P<0.01),Ⅱ组和Ⅲ组之间差异不显著(P>0.05);但仅有Ⅲ组H₅亚型禽流感疫苗的抗体水平极显著高于Ⅰ组(P<0.01),Ⅰ组和Ⅱ组之间差异不显著(P>0.05)。Ⅲ组血清中白细胞介素2含量显著或极显著高于Ⅰ组(P<0.05或P<0.01),但Ⅱ组与Ⅰ组差异不显著(P>0.05);血清中白细胞介素6含量3组间差异不显著(P>0.05)。Ⅲ组56日龄时的胸腺指数、脾脏指数和法氏囊指数均显著高于Ⅰ组(P<0.05),胸腺指数显著高于Ⅱ组(P<0.05),脾脏指数和法氏囊指数与Ⅱ组差异不显著(P>0.05)。由此可知,丁氨丙磷溶液能提高肉仔鸡平均日采食量和平均日增重,降低死亡率和降低发病率,但不影响料重比,同时可促进免疫器官发育,提高机体免疫功能;进口丁氨丙磷溶液的效果部分指标优于国产。

To study the effects of butafosfan solution on growth performance and immune function of broilers, a total of 180 one-day-old healthy yellow-feathered broilers were randomly divided into 3 groups with 6 replicates in each group and 10 broilers in each replicate. Control group (groupⅠ) was injected with saline solution, group Ⅱ was injected with domestic butafosfan solution, and group Ⅲ was injected with imported butafosfan solution. Experimental injection was carried out for three times, the first injection of 0.2 mL was at 1 day of age, the second injection of 0.5 mL was at 21 days of age, and the third injection of 1.0 mL was at 49 days of age. The experiment lasted for 56 days. The results showed as follows: 1) at 56 days of age, the average daily feed intake of groups Ⅱ and Ⅲwas significantly higher than that of group Ⅰ (P<0.05). The average daily gain of group Ⅱ was significantly higher compared with group Ⅰ (P<0.05), and group Ⅲ was significantly higher than group Ⅰ (P<0.01). However, the feed to gain ratio was not significantly different among the three groups (P>0.05). Disease incidence and mortality rate of groups Ⅱ and Ⅲ were significantly lower than those of group Ⅰ(P<0.01 and P<0.05). 2)At 28 and 56 days of age, the antibody level of Newcastle disease attenuated vaccine (clone 30) in serum of groups Ⅱ and Ⅲ was significantly higher than that of group Ⅰ (P<0.01), and there was no significant difference between group Ⅱ and group Ⅲ (P>0.05). But only the antibody level of H₅ subtype avian influenza of group Ⅲ was significantly higher than that of group Ⅰ (P<0.01), and there was no significant difference between group Ⅰ and group Ⅱ (P>0.05). Serum interleukin (IL-2) content of group Ⅲ was significantly higher than that of group Ⅰ (P<0.01 or P<0.05), but there was no significant difference between group Ⅰ and group Ⅱ (P>0.05), and there was no significant difference in serum IL-6 content among the three groups (P>0.05). The indexes of thymus, spleen and bursal of Fabricius of group Ⅲ were significantly higher than those of group Ⅰ at 56 days of age (P<0.05), thymus index was significantly higher than that of group Ⅱ (P<0.05), but there were no significant differences in the indexes of spleen and bursal of Fabricius compared with group Ⅱ (P>0.05). In conclusion, butafosfan solution can increase the average daily feed intake and the average daily gain of the broilers, and reduce the disease incidence and mortality rate, but can not affect feed to gain ratio. It can also promote the immune function and development of immune organs. The effects of imported butafosfan solution are better than those of domestic butafosfan solution.