水产营养 Aquaculture Nutrition

岩原鲤幼鱼的蛋白质需求量

  • 钱前 ,
  • 罗莉 ,
  • 白富瑾 ,
  • 张桂众 ,
  • 王朝明 ,
  • 朱根廷 ,
  • 颜忠 ,
  • 刘本详
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  • 1. 西南大学动物科技学院, 淡水鱼类资源与生殖发育教育部重点实验室, 水产科学重庆市市级重点实验室, 重庆 400715;
    2. 重庆市万州水产研究所, 重庆 404020

收稿日期: 2013-06-03

  网络出版日期: 2013-11-19

基金资助

中央高校基本科研业务费(XDJK2011C019);国家自然科学基金(NSFC31101909);重庆市自然基金(cstc2011jjA1570)

Dietary Protein Requirement of Juvenile Rock Carp (Procypris rabaudi)

  • QIAN Qian ,
  • LUO Li ,
  • BAI Fujin ,
  • ZHANG Guizhong ,
  • WANG Chaoming ,
  • ZHU Genting ,
  • YAN Zhong ,
  • LIU Benxiang
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  • 1. Key Laboratory of Freshwater Fish Reproduction and Development of Ministry of Education, Key Laboratory of Aquatic Science of Chongqing, College of Animal Science and Technology of Southwest University, Chongqing 400715, China;
    2. Wanzhou Fisheries Research Institute, Chongqing 404020, China

Received date: 2013-06-03

  Online published: 2013-11-19

摘要

本试验旨在确定岩原鲤幼鱼的蛋白质需求量。试验选用均重为(7.93±0.16) g的岩原鲤幼鱼540尾,随机分成6个组(每组3个重复,每个重复30尾),饲喂以明胶和酪蛋白为蛋白质源的蛋白质水平分别为25.1%、30.3%、34.8%、40.4%、44.8%、49.7%的纯化试验饲料98 d。结果表明:岩原鲤幼鱼的生长性能指标(增重率、特定生长率、饲料系数、肌肉RNA/DNA)、抗氧化指标 及肝功能指标 均在饲料蛋白质水平为40.4%时达到最佳值,除与饲料蛋白质水平为44.8%时的T-AOC差异不显著(P>0.05)外,与其他各组均有显著差异(P<0.05)。肠道蛋白酶、胰蛋白酶、脂肪酶活性随饲料蛋白质水平的增加呈先升后降的趋势,在40.4%组达到最高值,显著高于其他各组(P<0.05);而淀粉酶活性则反之。在40.4%组,全鱼粗蛋白质含量达最大值,显著高于除44.8%组外的其他各组(P<0.05);粗脂肪含量达最小值,显著低于其他各组(P<0.05);而全鱼水分和粗灰分含量不受饲料蛋白质水平的影响(P>0.05)。以增重率、饲料系数、肌肉RNA/DNA分别为效应指标与饲料蛋白质水平进行回归分析,得出岩原鲤幼鱼的蛋白质需求量为38.57%~40.25%。

本文引用格式

钱前 , 罗莉 , 白富瑾 , 张桂众 , 王朝明 , 朱根廷 , 颜忠 , 刘本详 . 岩原鲤幼鱼的蛋白质需求量[J]. 动物营养学报, 2013 , 25(12) : 2934 -2942 . DOI: 10.3969/j.issn.1006-267x.2013.12.021

Abstract

This experiment was conducted to estimate the dietary protein requirement of juvenile rock carp (procypris rabaudi). A total of 540 juvenile cock carp with an average body weight of (7.93±0.16) g were randomly allotted to 6 groups with 3 replicates each and each replicate contained 30 fish. Six purified diets formulated with casein and gelatin as protein sources containing graded protein levels of 25.1%, 30.3%, 34.8%, 40.4%, 44.8% and 49.7%, respectively. The experiment lasted for 98 days. The results showed as follows: at the protein level of 40.4%, the growth performance indices, antioxidant indices and liver function indices of the juvenile cock carp got the optimum values, and there were significant differences in those indices between 40.4% group and other groups (P<0.05) except T-AOC in 44.8% group (P>0.05). With the dietary protein level increasing, the activities of protease, trypsin and lipase of intestine were firstly increased and then decreased, and the highest values were all found in 40.4% group which significantly higher than other groups (P<0.05); however, the intestinal amylase activity was contrary to it. And at the protein level of 40.4%, the crude protein content in whole body got the maximum, and significantly higher than that in other groups except 44.8% group (P<0.05); the crude lipid content in whole body got the minimum, and significantly lower than that in other groups (P<0.05). The dietary protein level had no significant effects on moisture and ash contents in whole (P>0.05). The regression analysis between dietary protein level and WGR, SGR and muscle RNA/DNA of juvenile rock carp indicate that the dietary protein requirement of juvenile rock carp is 38.57% to 40.25%.

参考文献

[1] 丁瑞华.四川鱼类志[M].成都:四川科学技术出版社,1994.

[2] 施白南.岩原鲤的生活习性及其资源保护[J].西南师范学院学报:自然科学版,1980(2):93-103.

[3] 叶元土,罗莉,林仕梅,等.岩原鲤消化能力和营养价值的初步研究[J].四川动物,1998,17(1):7-10.

[4] 乐佩琦,陈宜渝.中国濒危动物红皮书(鱼类卷)[M].北京:科学出版社,1998.

[5] YUE H,YUAN H,ZHANG X Y.Fifteen novel polymorphic microsatellites in rock carp,Procypris rabaudi (Tchang),an endemic fish species in the upper reaches of the Yangtze river drainage[J].Conservation Genetics,2009,10(3):539-542.  

[6] ZHANG X Y,YUE B S,JIANG W X,et al.The complete mitochondrial genome of rock carp Procypris rabaudi (Cypriniformes:cyprinidae) and phylogenetic implications[J].Molecular Biology Reports,2009,36(5):981-991.  

[7] 谭国良,刘本祥,蒋明,等.岩原鲤人工繁殖技术研究[J].淡水渔业,2005,35(2):40-41.

[8] 林仕梅,罗莉.岩原鲤对7种饲料消化力离体研究[J].中国水产科学,2008,15(4):637-643.

[9] 李正友,黄辉,周路,等.岩原鲤肌肉营养成分分析[J].水生态学杂志,2009,2(1):126-127.

[10] 齐治家.生物化学[M].上海:上海技术出版社,1985.

[11] ABDEL-TAWWAB M,AHMAD M H,KHATTAB Y A E,et al.Effect of dietary protein level,initial body weight,and their interaction on the growth,feed utilization,and physiological alterations of Nile tilapia,Oreochromis niloticus(L.)[J].Aquaculture,2010,298(3/4):267-274.

[12] YANG S D,LIOU C H,LIU F G.Effects of dietary protein level on growth performance,carcass composition and ammonia excretion in juvenile silver perch(Bidyanus bidyanus)[J].Aquaculture,2002,213(1/2/3/4):363-372.

[13] COUTINHO F,PERES H,GUERREIRO I,et al.Dietary protein requirement of sharpsnout sea bream (Diplodus puntazzo,Cetti 1777)juveniles[J].Aquaculture,2012,356/357:391-397.

[14] 李勇,王雷,蒋克勇,等.水产动物营养的生态适宜与环保饲料[J].海洋科学,2004,28(3):76-78.

[15] 刁晓明,苏胜齐.一种具有养殖潜力的经济鱼类-岩原鲤[J].水产养殖,1993(6):22.

[16] 冯宪斌,杨德国,李茜,等.人工养殖条件下岩原鲤幼鱼排空率与摄食量[J].中国水产科学,2012,19(4):654-661.

[17] LEE J K,CHO S H,KIM K D,et al.Dietary protein requirement for young turbot (Scophthalmus maximus L.)[J].Aquaculture Nutrition,2003,9(4):283-286.  

[18] BROWN P B,DABROWSKI K,GARLING D L.Nutrition and feeding of yellow perch (Perca flavescens)[J].Applied Ichthyolgy,1996,12(3/4):171-174.

[19] MOHANTY S S,SAMANTARAY K.Effect of varying levels of dietary protein on the growth performance and feed conversion efficiency of snakehead Channa striata fry[J].Aquaculture,1996,2(2):89-94.

[20] 高婷婷,李勇,张家国,等.蛋白质在鱼虾免疫能力中的作用[J].动物营养学报,2011,23(9):1459-1465.

[21] YANG S D,LIOU C H,LIU F G.Effects of dietary protein level on growth performance,carcass composition and ammonia excretion in juvenile silver perch (Bidyanus bidyanus) [J].Aquaculture,2002,213:363-372.

[22] NG W K,ABDULLAH N,DE SILVA S S.The dietary protein requirement of the Malaysian mahseer,Tor tambroides (Bleeker),and the lack of protein-sparing action by dietary lipid[J].Aquaculture,2008,284(1/2/3/4):201-206.

[23] ESPINÓS F J,TOMAS A,PÉREZ L M,et al.Growth of dentex fingerlings (Dentex dentex) fed diets containing different levels of protein and lipid[J].Aquaculture,2003,218(1/2/3/4):479-490.

[24] 钱曦,王桂芹,周洪琪,等.饲料蛋白水平及豆粕替代鱼粉比例对翘嘴红鲌消化酶活性的影响[J].动物营养学报,2007,19(2):182-187.

[25] 石英,冷向军,李小勤,等.饲料蛋白水平对血鹦鹉幼鱼生长、体组成和肠道蛋白消化酶活性的影[J].水生生物学报,2009,33(5):874-880.

[26] 孙海涛,吉红.饲料蛋白水平对匙吻鲟幼鱼肌肉营养成分及消化酶活力的影响[J].水产科学,2011,30(12):721-725.

[27] UYS W,HECHT T,WALTERS M.Changes in digestive enzyme activities of Clarias gariepinus (Pisces:clariidae) after feeding[J].Aquaculture,1987,63(1/2/3/4):243-250.

[28] 蔡春芳,吴康,潘新法,等.蛋白质营养对异育银鲫生长和免疫力的影响[J].水生生物学报,2001,25(6):590-595.

[29] 张桐,徐奇友,许红,等.不同温度下不同蛋白水平对镜鲤(Cyprinus carpio L.)非特异性免疫的影响[J].东北农业大学学报,2010,41(12):80-85.

[30] 谷春梅,施用晖,乐国伟.过量食入蛋白对小鼠胰腺的氧化损伤[J].安徽农业科学,2010,38(25):13730-13731.

[31] KIRON V,WATANABE T,FUKUDA H,et al.Protein nutrition and defence mechanisms in rainbow trout Oncorhynchus mykiss[J].Comparative Biochemistry and Physiology Part A:Physiology,1995,111(3):351-359.  

[32] SHETH S G,FLAMM S L,GORDON F D,et al.AST/ALT ratio predicts cirrhosis in patients with chronic hepatitis C virus infection[J].The American Journal of Gastroenterology,1998,93(1):44-48.  

[33] 曹俊明,关国强,郑文晖,等.饲料蛋白质水平影响草鱼肝胰脏脂肪酸组成[J].中山大学学报:自然科学版,1997,36(5):64-68.

[34] WAES L V.Glutamate dehydmgenase:a reliable marker of live cell necrosis in clcoholie[J]. British Medical Journal,1997,2:1058-1060.

[35] 文华,高文,罗莉,等.草鱼幼鱼的饲料苏氨酸需要量[J].中国水产科学,2009,16(2):238-247.

[36] 汪福保,罗莉,文华,等.镁对草鱼生长、形体、肝功能和糖代谢的影响[J].淡水渔业,2011,41(2):57-62.

[37] OGINO C,SAITO K.Protein nutrition in fish.1.The utilization of dietary protein by young carp[J].Bulletin of the Japanese Society of Scientific Fisheries,1970,36(3):250-254.  
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