研究简报 Short Communications

孵化期注射维生素C对肉鸡生产性能、免疫和抗氧化性能的影响

  • 李世召 ,
  • 支丽慧 ,
  • 杨小军 ,
  • 姚军虎
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  • 西北农林科技大学动物科技学院, 杨陵 712100

收稿日期: 2013-06-15

  网络出版日期: 2013-11-19

基金资助

国家自然基金项目(31272464,31001017);教育部新世纪优秀人才(NCET-12-0476);陕西省科技新星基金(2012KJXX-18)

Vitamin C Injection during Incubation Period: Effects on Performance, Immune and Antioxidant Function of Broilers

  • LI Shizhao ,
  • ZHI Lihui ,
  • YANG Xiaojun ,
  • YAO Junhu
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  • College of Animal Science and Technology, Northwest A & F university, Yangling 712100, China

Received date: 2013-06-15

  Online published: 2013-11-19

摘要

本试验旨在研究种蛋孵化第11天注射不同水平维生素C对萨索肉鸡生产性能、免疫和抗氧化性能的影响。试验采用单因素完全随机设计,将180枚萨索肉鸡种蛋随机分为3个处理,每个处理4个重复,每个重复15枚。种蛋孵化第11天进行维生素C注射,剂量分别为0(注射0.1 mL生理盐水)、3和15 mg(0.1 mL的生理盐水溶液)。孵化21 d出雏后,每重复选取体重均匀、健康的雏鸡10只进行饲养,饲养期42 d。结果表明:1)3 mg注射组种蛋孵化率,肉鸡初生重、1~42日龄平均日采食量和料重比均显著高于其他组(P<0.05);2)维生素C注射组肉鸡21和42日龄的脾脏指数和法氏囊指数相对于生理盐水注射组均有一定程度的降低,但方差分析差异不显著(P>0.05);3)各组肉鸡21和42日龄外周血T淋巴细胞亚型比例均差异不显著(P>0.05);4)15 mg注射组肉鸡21日龄血浆总抗氧化能力显著高于3 mg注射组(P<0.05),但与生理盐水注射组差异不显著(P>0.05),42日龄血浆谷胱甘肽过氧化物酶和超氧化物歧化酶活性均显著高于其他组(P<0.05)。本试验结果显示,种蛋中注射3 mg维生素C可提高孵化率、肉鸡初生重和平均日采食量,但影响了料重比;注射15 mg维生素C能在一定程度上提高肉鸡的抗氧化性能。

本文引用格式

李世召 , 支丽慧 , 杨小军 , 姚军虎 . 孵化期注射维生素C对肉鸡生产性能、免疫和抗氧化性能的影响[J]. 动物营养学报, 2013 , 25(12) : 2998 -3005 . DOI: 10.3969/j.issn.1006-267x.2013.12.029

Abstract

This experiment was conducted to investigate the effects of different levels of vitamin C injection during incubation period at 11 embryonic ages on performance, immune and antioxidant function of Sasso broilers. The trial adopted one-factor completely random design. A total of 180 hatching eggs hatched by Sasso broiler breeders were randomly divided into 3 treatments with 4 replicates per treatment and 15 eggs in each replicate. The vitamin C injection was performed at 11 embryonic ages, with the doses of 0 (injecting saline), 3 and 15 mg (with 0.1 mL saline) per egg. After 21 days hatching, 10 healthy chicks with similiar body weight were selected from each replicate and then fed. The feeding period was 42 days. The results showed as follows: 1) compared with the other groups, injecting 3 mg vitamin C significantly increased the hatchability, birth weight, average daily feed intake and feed/gain of broilers aged 1 to 42 days (P<0.05). 2) Injecting vitamin C had no significant effects on spleen index and bursa of Fabricius index of broilers aged 21 and 42 days (P>0.05), but all had a decrease compared with injecting saline group numerically. 3) Vitamin C injection during incubation period had no significant effects on the lymphocyte subset of peripheral blood T lymphocyte of broilers aged 21 and 42 days (P>0.05). 4) Injecting 15 mg vitamin C significantly increased plasma total antioxidant capacity (T-AOC) of broilers aged 21 days compared with injecting 3 mg vitamin C (P<0.05), but there was no significant change compared with injecting saline (P>0.05); and injecting 15 mg vitamin C significantly increased plasma glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) activities of broilers aged 42 days compared with the other groups (P<0.05). Based on the results of the experiment, it is concluded thats injecting 3 mg vitamin C in hatching eggs at 11 embryonic ages can improve hatchability, birth weight and average daily feed intake of broilers, but decrease feed conversion rate. The antioxidant function may have an increase partly when the dose of vitamin C injection is 15 mg.

参考文献

[1] 谢宝柱.新型饲料添加剂茶多酚的研究[J].中国饲料添加剂,2005(8):17-20.

[2] PARDUE S L,THAXTON J P.Ascorbic acid in poultry:a review[J].World's Poulty Science,1986,42(2):107-123.  

[3] STEPHEN J,WEISS S J.Tissue destruction by neutrophils[J].New England Journal of Medicine,1989,320(6):365-376.  

[4] NJOKU P C.Effect of dietary ascorbic acid (vitamin C) supplementation on the performance of broiler chickens in a tropical environment[J].Animal Feed Science and Technology,1986,16(1):17-24.

[5] 李彦,杨在宾,杨维仁,等.日粮中添加不同水平维生素C对肉鸡抗氧化和免疫性能影响的研究[J].山东农业大学学报:自然科学版,2009,40(2):229-234.

[6] NOWACZEWSKI S,KONTECKA H,KRYSTIANIAK S.Effect of in ovo injection of vitamin C during incubation on hatchability of chickens and ducks[J].Folia Biologica,2012,60(1/2):93-97.

[7] UNI Z,FERKET P R,TAKO E,et al.In ovo feeding improves energy status of late-term chicken embryos[J].Poultry Science,2005,84(5):764-770.

[8] 刘梅英,陈伟,彭鹏,等.胚蛋注射在家禽营养中的研究进展[J].中国家禽,2007,29(21):40-42.

[9] AL-DARAJI H J,AL-MASHADANI A A,AL-HAYANI W K,et al.Effect of in ovo injection with L-arginine on productive and physiological traits of Japanese quail[J].South African Journal of Animal Science,2012,42(2):139-145.

[10] KORNASIO R,HALEVY O,KEDAR O,et al.Effect of in ovo feeding and its interaction with timing of first feed on glycogen reserves,muscle growth,and body weight[J].Poultry Science,2011,90(7):1467-1477.  

[11] BAKYARAJ S,BHANJA S K,MAJUMDAR S,et al.Modulation of post-hatch growth and immunity through in ovo supplemented nutrients in broiler chickens[J].Journal of the Science of Food and Agriculture,2012,92(2):313-320.  

[12] 朱旭东,黄建文,肖养善.法国萨索优质肉鸡的引进及推广[J].养禽与禽病防治,2007(6):20-22.

[13] IPEK A,SAHAN U,YILMAZ B,et al.The effect of in ovo ascorbic acid and glucose injection in broiler breeder eggs on hatchability and chick weight[J].Archiv FÜr GeflÜgelkunde,2004,68(3):132-135.  

[14] OHTA Y,KIDD M T.Optimum site for in ovo amino acid injection in broiler breeder eggs[J].Poultry Science,2001,80(10):1425-1429.

[15] ELÍBOL O,TVRKOLU M,AKAN M,et al.Effects of Ascorbic acid injection during incubation on the hatchability of large broiler eggs[J].Turkish Journal of Veterinary and Animal Sciences,2001,25(3):245-248.

[16] TULLETT S G.Science and the art of incubation[J].Poultry Science,1990,69(1):1-15.  

[17] ZAKARIA A H,AL-ANEZI M A.Effect of ascorbic acid and cooling during egg incubation on hatchability,culling,mortality,and the body weights of broiler chickens[J].Poultry Science,1996,75(10):1204-1209.  

[18] WILSON J X,JAWORSKI E M.Effect of oxygen on ascorbic acid uptake and concentration in embryonic chick brain[J].Neurochemical Research,1992,17(6):571-576.  

[19] MEGLASSON M D,HAZELWOOD R L.Ascorbic acid diabetogenesis in the domestic fowl[J].General and Comparative Endocrinology,1982,47(2):205-212.  

[20] INGRAM D R,FLOYD S A,BARR J W,et al.Influence of in ovo injection of glucose on subsequent body weight[J].Poultry Science 86th Annual Meeting Abstract,1997,76(Suppl.1):51.

[21] 黄仁录,张振红,陈培高,等.大豆低聚糖对肉鸡抗氧化能力和免疫功能的影响[J].河北农业大学学报,2006,29(6):87-90.

[22] 栾维民.鸡外周免疫器官中淋巴细胞发育的研究[D].博士学位论文.长春:吉林农业大学,2008.

[23] 王绍琛,刘迎春,高怀涛,等.鸡胚免疫器官组织学发育过程观察[J].中国兽医科技,2004,34(8):57-61.

[24] 李淑芳,李英,张继东,等.米糠多糖对雏鸡T细胞免疫功能的影响[J].养禽与禽病防治,2009(3):14-16.

[25] 孙永科,田占成,王云峰,等.表达鸡γ-干扰素和传染性支气管病毒S1基因的重组鸡痘病毒疫苗对鸡外周血T淋巴细胞动态分布影响的研究[J].中国预防兽医学报,2006,28(5):539-543.

[26] 任莉,谢伟才,冯定远,等.日粮中添加维生素C对肉鸡生产性能和免疫性能的影响[J].黑龙江畜牧兽医,2011(9):58-59.

[27] 王显慧,刘福柱,牛竹叶,等.VC对肉鸡生产性能、胴体品质、免疫机能和抗氧化特性的影响[J].西北农林科技大学学报:自然科学版,2010,38(2):1-7.

[28] MCCORKLE F,TAYLOR R,STINSON R,et al.The effects of a megalevel of vitamin C on the immune response of the chicken[J].Poultry Science,1980,59(6):1324-1327.  

[29] 李新花,王晓霞,褚耀诚.日粮维生素E、C的添加水平对AA肉仔鸡生产性能及血清抗氧化酶活力的影响[J].北京农学院学报,2006,21(1):33-37.
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