动物营养学报 >

2013 , Vol. 25 >Issue 12: 3033 - 3040

DOI: https://doi.org/10.3969/j.issn.1006-267x.2013.12.034

研究简报 Short Communications

饲料中添加β-葡聚糖对花鲈生长性能、体成分、血清生化指标和抗氨氮应激能力的影响

  • 吴春玉 ,
  • 曹俊明 ,
  • 黄燕华 ,
  • 王国霞 ,
  • 赵红霞 ,
  • 刘小玲 ,
  • 付晶晶 ,
  • 李林
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  • 1. 华中农业大学水产学院, 武汉 430070;
    2. 广东省农业科学院动物科学研究所, 广州 510640;
    3. 广东省动物育种与营养公共实验室, 广州 510640;
    4. 广东省畜禽育种与营养研究重点实验室, 广州 510640

收稿日期: 2013-06-21

  网络出版日期: 2013-11-19

基金资助

广东省自然科学基金研究团队项目(10351064001000000);广东省海洋渔业局科技推广专项(A201101D05);广东省科技型中小企业技术创新基金项目(2012CY002)

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Effects of Dietary β-Glucan on Growth Performance, Body Composition, Serum Biochemical Indices and Anti-Ammonia-Nitrogen Stress Ability of Japanese Seabass (Lateolabrax japonicus)

  • WU Chunyu ,
  • CAO Junming ,
  • HUANG Yanhua ,
  • WANG Guoxia ,
  • ZHAO Hongxia ,
  • LIU Xiaoling ,
  • FU Jingjing ,
  • LI Lin
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  • 1. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China;
    2. Institute of Animal Science, Guangdong Academy of Agricultural Science, Guangzhou 510640, China;
    3. Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China;
    4. Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, China

Received date: 2013-06-21

  Online published: 2013-11-19

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摘要

本试验旨在研究饲料中添加β-葡聚糖对花鲈生长性能、体成分、血清生化指标和抗氨氮应激能力的影响。选用体重为(8.35±0.17) g的花鲈(Lateolabrax japonicus)720尾,随机分为6组,每组4个重复,每个重复30尾鱼。对照组投喂基础饲料,β-葡聚糖添加组分别投喂在基础饲料中添加200、400、600、800和1 000 mg/kg β-葡聚糖的试验饲料,饲养6周后,应用氯化铵进行氨氮应激试验。结果表明:与对照组相比,各β-葡聚糖添加组花鲈的增重率和特定生长率显著升高(P<0.05),饲料系数显著降低(P<0.05);各组之间存活率、肥满度、肝体指数差异不显著(P>0.05)。各组花鲈鱼体的粗蛋白质、粗脂肪、粗灰分、水分含量差异不显著(P>0.05)。饲料中添加β-葡聚糖对花鲈血清谷丙转氨酶活性以及总蛋白、高密度脂蛋白、低密度脂蛋白、胆固醇、甘油三酯、尿素氮含量均无显著影响(P>0.05)。氨氮应激96 h,400和600 mg/kg β-葡聚糖添加组的累计死亡率与对照组相比显著降低(P<0.05)。由此得出,饲料中添加β-葡聚糖可提高花鲈的生长性能以及抗氨氮应激能力;以增重率为评价指标,运用抛物线与折线模型相结合分析得出花鲈饲料中β-葡聚糖的适宜添加量为415.10 mg/kg。

关键词： 花鲈; β-葡聚糖; 生长性能; 体成分; 血清生化指标; 氨氮应激

本文引用格式

吴春玉 , 曹俊明 , 黄燕华 , 王国霞 , 赵红霞 , 刘小玲 , 付晶晶 , 李林 . 饲料中添加β-葡聚糖对花鲈生长性能、体成分、血清生化指标和抗氨氮应激能力的影响[J]. 动物营养学报, 2013 , 25(12) : 3033 -3040 . DOI: 10.3969/j.issn.1006-267x.2013.12.034

Abstract

This experiment was conducted to investigate the effects of dietary β-glucan on growth performance, body composition, serum biochemical indices and anti-ammonia-nitrogen stress ability of Japanese seabass (Lateolabrax japonicus). A total of 720 Japanese seabass with the body weight of (8.35±0.17) g were randomly divided into 6 groups with 4 replicates per group and 30 fish per replicate. The fish in control group were fed a basal diet, and the fish in β-glucan supplemental groups were fed the experimental diets supplemented with 0, 200, 400, 600, 800 and 1 000 mg/kg β-glucan on the basis of basal diet, respectively. After 6 weeks feeding, ammonia chloride was used to conduct a ammonia-nitrogen stress test in each group. The results showed as follows: compared with the control group, the weight gain rate and specific growth rate in the β-glucan supplemental groups were significantly increased (P<0.05), and the feed conversation ratio was significantly decreased (P<0.05), but the condition factor, survival rate and hepatosomatic index had no significant difference (P>0.05). No significant differences were found among all groups in the crude protein, crude lipid, ash and moisture contents of whole body (P>0.05). Dietary β-glucan had no significant effects on serum alanine transaminase activity, and high density lipoprotein, low density lipoprotein, cholesterol, triglycerides and urea nitrogen contents (P>0.05). After 96 h ammonia-nitrogen stress, the cumulative mortality rate in the 400 and 600 mg/kg β-glucan supplemental groups was significantly decreased when compared with the control group (P<0.05). In conclusion, dietary β-glucan can increase the growth performance and anti-ammonia-nitrogen stress ability. Using weight gain rate as a evaluation index, the combination of parabola and broken-line regression analysis shows that the optimal supplemental level of β-glucan in the diet of Japanese seabass is 415.10 mg/kg.

Key words： Japanese seabass (Lateolabrax japonicus); β-glucan; growth performance; body composition; serum biochemical indices; ammonia-nitrogen stress

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