综述 Review

赭曲霉毒素A的肠毒性及其营养干预

  • 范斌 ,
  • 余冰 ,
  • 王乐成 ,
  • 田刚
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  • 1. 四川农业大学动物营养研究所, 成都 611130;
    2. 动物抗病营养教育部重点实验室, 成都 611130

收稿日期: 2013-09-13

  网络出版日期: 2014-01-27

基金资助

科技部科技成果转化项目“饲料霉菌毒素体内解毒技术的开发与应用(2012GB2F000399)”;四川农业大学“双支计划”项目

Intestinal Toxicity of Ochratoxin A and Its Nutritional Intervention

  • FAN Bin ,
  • YU Bing ,
  • WANG Lecheng ,
  • TIAN Gang
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  • 1. Institue of Animal Nutrition, Sichuan Agricultural University, Chengdu 611130, China;
    2. Key Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2013-09-13

  Online published: 2014-01-27

摘要

赭曲霉毒素A(ochratoxin A,OTA)是一类主要由曲霉属和青霉属等产毒真菌产生的真菌毒素,广泛存在于各种粮食作物及其副产品中,是食品和饲料原料的重要污染物,其广泛的毒理病理学效应对动物生产和人类健康构成了极大的潜在危害,已引起了人们的高度关注。肠道既是OTA等外源有毒有害物质的主要吸收部位,又是阻挡其进入体循环的第1道物理屏障,同时还可能是其发挥毒性效应的靶器官。因此,本文就OTA的肠毒性及其作用机制、营养对OTA肠毒性的缓解效应做简要综述,以期为缓解OTA的肠毒性提供理论基础和实践依据。

本文引用格式

范斌 , 余冰 , 王乐成 , 田刚 . 赭曲霉毒素A的肠毒性及其营养干预[J]. 动物营养学报, 2014 , 26(2) : 334 -341 . DOI: 10.3969/j.issn.1006-267x.2014.02.007

Abstract

Ochratoxin A (OTA) is a mycotoxins produced by toxigenic fungi, especially some species of Aspergillus and Penicillium. OTA widely exists in food crops and their by-products, which is known as an important contaminant of food and feed materials. Toxicological pathology studies showed that OTA had a great potential threat to animal reproduction and human health, which had resulted in the public attention highly. The intestine not only is the main absorption site of the toxic exogenous, such as OTA, but also is an organ where the exogenous toxins are blocked into the systemic circulation as the first physical barrier, meanwhile, the intestine may be the target organ suffered from the toxicity hazard. This article focused on the intestinal toxicity of OTA and its mechanisms, as well as its nutritional intervention, in order to provide theoretical foundation and practice basis for relieving intestinal toxicity of OTA.

参考文献

[1] DUARTE S C, LINO C M, PENA A.Ochratoxin A in feed of food-producing animals:an undesirable mycotoxin with health and performance effects[J].Veterinary Microbiology, 2011, 154(1/2):1-13.

[2] RINGOT D, CHANGO A, SCHNEIDER Y, et al.Toxicokinetics and toxicodynamics of ochratoxin A, an update[J].Chemico-Biological Interactions, 2006, 159(1):18-46.  

[3] HADJEBA-MEDJDOUB K, TOZLOVANU M, PFOHL-LESZKOWICZ A, et al.Structure-activity relationships imply different mechanisms of action for ochratoxin A-mediated cytotoxicity and genotoxicity[J].Chemical Research in Toxicology, 2012, 25(1):181-190.  

[4] HADIDANE R, BACHA H, CREPPY E E, et al.Isolation and structure determination of natural analogues of the mycotoxin ochratoxin A produced by Aspergillus ochraceus[J].Toxicology, 1992, 76(3):233-243.  

[5] KUMAGAI S, AIBARAI K.Intestinal absorption and secretion of ochratoxin A in the rat[J].Toxicology and Applied Pharmacology, 1982, 64(1):94-102.  

[6] KUMAGAI S.Effects of plasma ochratoxin A and luminal pH on the jejunal absorption of ochratoxin A in rats[J].Food and Chemical Toxicology, 1988, 26(9):753-758.  

[7] BERGER V, GABRIEL A F, SERGENT T, et al.Interaction of ochratoxin A with human intestinal Caco-2 cells:possible implication of a multidrug resistance-associated protein (MRP2)[J].Toxicology Letters, 2003, 140/141:465-476.

[8] GALTIER P, ALVINERIE M, CHARPENTEAU J L.The pharmacokinetic profiles of ochratoxin A in pigs, rabbits and chickens[J].Food and Cosmetics Toxicology, 1981, 19:735-738.

[9] HAGELBERG S, HULT K, FUCHS R.Toxicokinetics of ochratoxin A in several species and its plasma-binding properties[J].Journal of Applied Toxicology, 1989, 9(2):91-96.  

[10] 高翔, 李梅, 张立实.赭曲霉毒素A的毒性研究进展[J].国外医学:卫生学分册, 2005, 32(1):51-55.

[11] SUZUKI S, SATOH T, YAMAZAKI M.The pharmacokinetics of ochratoxin A in rats[J].The Japanese Journal of Pharmacology, 1977, 27(5):735-744.  

[12] SZCZECH G M, CARLTON W W, TUITE J, et al.Ochratoxin A toxicosis in swine[J].Veterinary Pathology, 1973, 10(4):347-364.  

[13] SZCZECH G M, CARLTON W W, TUITE J.Ochratoxicosis in beagle dogs.Ⅱ.Pathology[J].Veterinary Pathology, 1973, 10(3):219-231.  

[14] KITCHEN D N, CARLTON W W, TUITE J.Ochratoxin A and citrinin induced nephrosis in beagle dogs Ⅱ.Pathology[J].Veterinary Pathology, 1977, 14(3):261-272.  

[15] KANISAWA M, SUZUKI S, KOZUKA Y, et al.Histopathological studies on the toxicity of ochratoxin A in rats.Ⅰ.Acute oral toxicity[J].Toxicology and Applied Pharmacology, 1977, 42(1):55-64.  

[16] KANISAWA M, SUZUKI S, MOROI K.The mode of action of ochratoxin A in acute enteritis in rats[J].Journal of Environmental Pathology Toxicology and Oncology, 1990, 10(1/2):56-63.

[17] WARREN M F, HAMILTON P B.Intestinl fragility during ochratoxicosis and aflatoxicosis in broiler chickens[J].Applied and Environmental Microbiology, 1980, 40(3):641-645.

[18] FUKATA T, SASAI K, BABA E, et al.Effect of ochratoxin A on Salmonella typhimurium-challenged layer chickens[J].Avian Diseases, 1996, 40(4):924-926.  

[19] KUMAR A, JINDAL N, SHUKLA C L, et al.Effect of ochratoxin A on Escherichia coli-challenged broiler chicks[J].Avian Diseases, 2003, 47(2):415-424.  

[20] KUMAR A, JINDAL N, SHUKLA C L, et al.Pathological changes in broiler chickens fed ochratoxin A and inoculated with Escherichia coli[J].Avian Pathology, 2004, 33(4):413-417.  

[21] MANAFI M, MOHAN K, ALI M N.Effect of ochratoxin A on coccidiosis-challenged broiler chicks[J].World Mycotoxin Journal, 2011, 4(2):177-181.  

[22] MARESCA M, MAHFOUD R, PFOHL-LESZKOWICZ A, et al.The mycotoxin ochratoxin A alters intestinal barrier and absorption functions but has no effect on chloride secretion[J].Toxicology and Applied Pharmacology, 2001, 176(1):54-63.  

[23] 陈平, 田刚, 陈代文, 等.赭曲霉毒素A对IPEC-J2细胞生长和氧化还原平衡的影响[J].中国畜牧杂志, 2011, 47(3):22-26.

[24] ALEO M D, WYAT R D, SCHNELLMANN R G.Mitochondrial dysfunction is an early event in ochratoxin A but not oosporein toxicity to rat renal proximal tubules[J].Toxicology and Applied Pharmacology, 1991, 107(1):73-80.  

[25] AL-ANATI L, PETZINGER E.Immunotoxic activity of ochratoxin A[J].Journal of Veterinary Pharmacology and Therapeutics, 2006, 29(2):79-90.  

[26] 姚瑶, 王琳琳.白细胞介素-8研究进展[J].实用儿科临床杂志, 2009, 24(10):789-792.

[27] MARESCA M, YAHI N, YOUNS-SAKR L, et al.Both direct and indirect effects account for the pro-inflammatory activity of enteropathogenic mycotoxins on the human intestinal epithelium:stimulation of interleukin-8 secretion, potentiation of interleukin-1β effect and increase in the transepithelial passage of commensal bacteria[J].Toxicology and Applied Pharmacology, 2008, 228(1):84-92.  

[28] BOUHET S, LE DORZE E, PERES S, et al.Mycotoxin fumonisin B1 selectively down-regulates the basal IL-8 expression in pig intestine:in vivo and in vitro studies[J].Food and Chemical Toxicology, 2006, 44(10):1768-1773.  

[29] MCLAUGHLIN J, PADFIELD P J, BURT J P, et al.Ochratoxin A increases permeability through tight junctions by removal of specific claudin isoforms[J].American Journal of Physiology:Cell Physiology, 2004, 287(5):C1412-C1417.

[30] RANALDI G, MANCINI E, FERRUZZA S, et al.Effects of red wine on ochratoxin A toxicity in intestinal Caco-2/TC7 cells[J].Toxicology in Vitro, 2005, 21(2):204-210.

[31] LAMBERT D, PADFIELD P J, MCLAUGHLIN J, et al.Ochratoxin A displaces claudins from detergent resistant membrane microdomains[J].Biochemical and Biophysical Research Communications, 2007, 358(2):632-636.  

[32] MARIN-KUAN M, NESTLER S, VERGUET C, et al.A toxicogenomics approach to identify new plausible epigenetic mechanisms of ochratoxin a carcinogenicity in rat[J].Toxicological Sciences, 2006, 89(1):120-134.

[33] CAVIN C, DELATOUR T, MARIN-KUAN M, et al.Reduction in antioxidant defenses may contribute to ochratoxin A toxicity and carcinogenicity[J].Toxicological Sciences, 2007, 96(1):30-39.

[34] BOESCH-SAADATMANDI C, LOBODA A, JOZKOWICZ A, et al.Effect of ochratoxin A on redox-regulated transcription factors, antioxidant enzymes and glutathione-S-transferase in cultured kidney tubulus cells[J].Food and Chemical Toxicology, 2008, 46(8):2665-2671.  

[35] BOESCH-SAADATMANDI C, WAGNER A E, GRAESER A C, et al.Ochratoxin A impairs Nrf2-dependent gene expression in porcine kidney tubulus cells[J].Journal of Animal Physiology and Animal Nutrition, 2009, 93(5):547-554.  

[36] TAGUCHI K, MOTOHASHI H, YAMAMOTO M.Molecular mechanisms of the Keap1-Nrf2 pathway in stress response and cancer evolution[J].Genes to Cells, 2011, 16(2):123-140.  

[37] KHOR T O, HUANG M T, KWON K H, et al.Nrf2-deficient mice have an increased susceptibility to dextran sulfate sodium-induced colitis[J].Cancer Research, 2006, 66(24):11580-11584.  

[38] 陈平.赭曲霉毒素A对IPEC-J2细胞Nrf2抗氧化系统的影响及硒的保护效应[D].硕士学位论文.雅安:四川农业大学, 2010.

[39] RANALDI G, CAPRINI V, SAMBUY Y, et al.Intracellular zinc stores protect the intestinal epithelium from ochratoxin A toxicity[J].Toxicology in Vitro, 2009, 23(8):1516-1521.  
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