饲粮棉籽粕水平对高峰期蛋鸭产蛋性能、蛋品质、血浆生化指标、卵巢形态及棉酚残留的影响

阮栋; 林映才; 张罕星; 陈伟; 王爽

doi:10.3969/j.issn.1006-267x.2014.02.010

动物营养学报 >

2014 , Vol. 26 >Issue 2: 353 - 362

DOI: https://doi.org/10.3969/j.issn.1006-267x.2014.02.010

禽营养 Poultry Nutrition

饲粮棉籽粕水平对高峰期蛋鸭产蛋性能、蛋品质、血浆生化指标、卵巢形态及棉酚残留的影响

阮栋 ,
林映才 ,
张罕星 ,
陈伟 ,
王爽

展开

广东省农业科学院动物科学研究所, 畜禽育种国家重点实验室, 农业部动物营养与饲料(华南)重点开放实验室, 广东省动物育种与营养公共实验室, 广东省畜禽育种与营养研究重点实验室, 广州 510640

收稿日期: 2013-09-02

网络出版日期: 2014-01-27

基金资助

现代农业产业技术体系建设资金资助（CARS-43-13）

收起

Effects of Dietary Cottonseed Meal Level on Laying Performance, Egg Quality, Plasma Biochemical Parameters, Ovarian Morphology and Gossypol Residue of Laying Ducks at Peak Production

RUAN Dong ,
LIN Yingcai ,
ZHANG Hanxing ,
CHEN Wei ,
WANG Shuang

Expand

State Key Laboratory of Livestock and Poultry Breeding, Key Laboratory of Animal Nutrition and Feed Science (South China) of Ministry of Agriculture, Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Science, Guangzhou 510640, China

Received date: 2013-09-02

Online published: 2014-01-27

Fold

摘要

本试验旨在研究饲粮棉籽粕（CSM）水平对高峰期蛋鸭产蛋性能、蛋品质、血浆生化指标、卵巢形态及棉酚残留的影响，以探讨饲粮中CSM适宜水平。试验选用19周龄龙岩山麻鸭720只，采用单因子随机分组试验设计，随机分为6组，每组4个重复，每个重复30只鸭。各组饲粮CSM水平分别为0（对照）、4.5%、9.0%、13.5%、18.0%和22.5%，CSM中游离棉酚（FG）实测值为266 mg/kg，试验期3个月。结果表明：1）与对照组相比，13.5%、18.0%和22.5%组显著降低了平均蛋重（P<0.05），22.5%组显著降低了平均蛋重和日产蛋重并提高了料蛋比（P<0.05）。2）饲粮CSM水平对高峰期蛋鸭产蛋率、破蛋率、畸形蛋率、蛋壳厚度、蛋壳强度、哈氏单位及蛋黄颜色无显著影响（P>0.05）。3）与对照组相比，9.0%、13.5%、18.0%和22.5%组显著降低了血浆还原型谷胱甘肽（GSH）含量和GSH/氧化型谷胱甘肽（GSSG）值，并提高了丙二醛（MDA）含量（P<0.05）；各组间GSSG含量、总抗氧化能力（T-AOC）及谷草转氨酶（GOT）、谷丙转氨酶（GPT）和碱性磷酸酶（ALP）活性均差异不显著（P>0.05）。4）9.0%、13.5%、18.0%和22.5%组可明显破坏优势卵泡的完整性，卵泡变形破裂，局部呈融溶状。5）22.5%组试验鸭直肠内容物中FG含量为4.55 mg/kg，胸肌、肝脏、肾脏及蛋黄、蛋清中并未检测出FG残留。综上所述，饲粮CSM水平低于9.0%时不影响高峰期蛋鸭产蛋性能、蛋品质，组织无损伤，胸肌及蛋中无棉酚残留。

关键词： 棉籽粕; 蛋鸭; 产蛋性能; 蛋品质; 血浆生化指标; 卵巢形态; 棉酚

本文引用格式

阮栋 , 林映才 , 张罕星 , 陈伟 , 王爽 . 饲粮棉籽粕水平对高峰期蛋鸭产蛋性能、蛋品质、血浆生化指标、卵巢形态及棉酚残留的影响[J]. 动物营养学报, 2014 , 26(2) : 353 -362 . DOI: 10.3969/j.issn.1006-267x.2014.02.010

Abstract

This experiment was conducted to study the effects of cottonseed meal (CSM) on laying performance, egg quality, plasma biochemical parameters, ovarian morphology and gossypol residue of laying ducks at peak production, and to estimate the dietary CSM optimal level of laying ducks. A single factor design was adopted and seven hundred and twenty 19-week-old Longyan laying ducks were randomly divided into 6 groups with 4 replicates per group and 30 ducks per replicate. Ducks in the six groups were fed the basal diet supplemented with 0 (control), 4.5%, 9.0%, 13.5%, 18.0% and 22.5% CSM, respectively. The content of free gossypol (FG) in CSM was 266 mg/kg. This experiment lasted for 12 weeks. The results showed as follows: 1) compared with the control group, the average egg weight in 13.5%, 18.0% and 22.5% groups was significantly decreased (P<0.05), and the average egg weight and daily egg mass in 22.5% group were significantly decreased (P<0.05), but the ratio of feed to egg in 22.5% group was significantly increased (P<0.05). 2) Dietary CSM level had no significant effects on laying rate, broken egg rate, abnormal egg rate, eggshell thickness, eggshell strength, Haugh unit and yolk color (P>0.05). 3) Compared with the control group, the plasma reduced glutathione (GSH) content and the GSH/oxidized glutathione (GSSG) value in 9.0%, 13.5%, 18.0% and 22.5% groups were significantly decreased (P<0.05), but the plasma malondialdehyde (MDA) content was significantly increased (P<0.05). There were no differences in total antioxidant capacity (T-AOC) and the activities of glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT) and alkaline phosphatase (ALP) among all groups (P>0.05). 4) The integrity of the dominant follicles was impaired, and some of them were fractured to fusion in 9.0%, 13.5%, 18.0% and 22.5% groups. 5) The content of FG in rectal contents was 5.57 mg/kg in 22.5% group, but it was undetectable in breast muscle, liver, kidney, egg yolk and egg albumen. In conclusion, there is no negative effects on laying performance, egg quality and organic damage when dietary CSM level is less than 9.0%, and there is no residues of gossypol in egg and breast muscle.

Key words： cottonseed meal; laying ducks; laying performance; egg quality; plasma biochemical parameters; ovarian morphology; gossypol

参考文献

[1] 王利, 汪开毓.动物棉酚中毒的研究进展[J].畜禽业, 2002(5):26-28.

[2] KOYACIC P.Mechanism of drug and toxic actions of gossypol: focus on reactive oxygen species and electron transfer[J].Current Medicinal Chemistry, 2003, 10(24):2711-2718.

[3] BASINI G, BUSSOLATI S, BAIONI L, et al.Gossypol, a polyphenolic aldehyde from cotton plant, interferes with swine granulose cell function[J].Domestic Animal Endocrinology, 2009, 37(1):30-36.

[4] GAMBOA D A, CALHOUN M C, KUHLMANN S W, et al.Tissue distribution of gossypol enantiomers in broilers fed various cottonseed meals[J].Poultry Science, 2001, 80(7):920-925.

[5] GAMBOA D A, CALHOUN M C, KUHLMANN S W, et al.Use of expander cottonseed meal in broiler diets formulated on a digestible amino acid basis[J].Poultry Science, 2001, 80(6):789-794.

[6] 杨茹洁.可消化AA平衡的高棉粕饲粮对蛋鸡的生产性能、健康状况及蛋品质的影响[D].硕士学位论文.太谷:山西农业大学, 2003.

[7] ÖZDO?AN M, WELLMANN K, PAKSUZ E.Effect of gossypol on blood serum parameters and small intestinal morphology of male broilers[J].Journal of Animal Physiology and Animal Nutrition, 2012, 96(1):95-101.

[8] KIM H L, CALHOUN M C.Determination of gossypol in plasma and tissues of animals[J].Inform, 1995, 6:486.

[9] 李建国, 王建华.游离棉酚水平对海兰蛋鸡生产性能的影响[J].家畜生态学报, 2007, 28(6):31-33.

[10] LORDELO M M, CALHOUN M C, DALE N M, et al.Relative toxicity of gossypol enantiomers in laying and broiler breeder hens[J].Poultry Science, 2007, 86(3):582-590.

[11] 周顺伍.动物生物化学[M].北京:中国农业出版社, 1999.

[12] BRAHAM J E, JARQUÍN R, ELÍAS L G, et al.Effect of calcium and gossypol on the performance of swine and on certain enzymes and other blood constituents[J].The Journal of Nutrition, 1967, 91(1):47-54.

[13] 尹逊慧.棉籽粕在樱桃谷肉鸭饲粮中应用技术研究[D].硕士学位论文.广州:华南农业大学, 2009.

[14] 曾秋凤, 柏鹏.棉酚在肉禽体内的毒性和残留及其营养对策的研究进展[J].动物营养学报, 2013, 25(5):917-922.

[15] ALI S F, El SEWEDY S M.Effect of gossypol on liver metabolic enzymes in male rats[J].Toxicology Letters, 1984, 23(3):299-306.

[16] 倪鸣, 袁慧.醋酸棉酚对小鼠黄体细胞凋亡的影响[C]//中国畜牧兽医学会家畜内科学分会2009年学术研讨会论文集.北京:中国畜牧兽医学会, 2009:506-512.

[17] 赵萍.棉籽壳对雄性细毛羊血清、肝脏抗氧化指标的影响[J].草食家畜, 2011(4): 44-47.

[18] 刘锦湖.日粮中的游离棉酚对蛋鸡生产性能及鸡蛋储存品质的影响[D].硕士学位论文.北京:中国农业大学, 2006.

[19] 高布潜, 姜洪, 尹进.防止鸡蛋变色和失重的保藏方法——蛋鸡饲喂棉籽饼粕引起鸡蛋变色是氧气在起主导作用[J].中国粮油学报, 1988(2):62-64.

[20] 王冬梅, 郭书贤, 梁运祥.棉子饼粕中棉酚的危害与脱毒方法研究进展[J].湖北农业科学, 2009, 48(2):487-493.

[21] ABOU-DONIA M B.Physiological effects and metabolism of gossypol[J].Residue reviews, 1976, 61:125-160.

[22] LORDELO M M, DAVIS A J, CALHOUN M C, et al.Relative toxicity of gossypol enantiomers in broilers[J].Poultry Science, 2005, 84(9):1376-1382.

[23] 吕云峰, 王修启, 赵青余, 等.棉酚在饲料中安全限量及畜产品中残留研究进展[J].中国农学通报, 2010, 26(24):1-5.

[24] 龙安梅.醋酸棉酚对猪原代培养黄体细胞凋亡影响的研究[D].硕士学位论文.长沙:湖南农业大学, 2006.

[25] OLIVER C L, MIRANDA M B, SHANGARY S, et al.(-)-Gossypol acts directly on the mitochondria to overcome Bcl-2-and Bcl-XL-mediated apoptosis resistance[J].Molecular Cancer Therapeutics, 2005, 4(1):23-31.

[26] XU L, YANG D J, WANG S M, et al.Gossypol enhances response to radiation therapy and results in tumor regression of human prostate cancer[J].Molecular Cancer Therapeutics, 2005, 4(2):197-205.

[27] 陈文飞, 付帅, 岳万远, 等.醋酸棉酚对人舌鳞癌Tca8113细胞凋亡及DNA甲基转移酶1表达的影响[J].肿瘤, 2013, 33(5):404-408.

Options

文章导航

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献

关于本刊

在线期刊

作者中心

相关单位

联系我们